{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,5]],"date-time":"2026-02-05T09:46:28Z","timestamp":1770284788022,"version":"3.49.0"},"reference-count":9,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2013,9,1]],"date-time":"2013-09-01T00:00:00Z","timestamp":1377993600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Vaccine Immunol"],"published-print":{"date-parts":[[2013,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>Previous reports have shown that the sensitivity of the 6-day lymphocyte stimulation test is much higher than those of commercially available gamma interferon release assays (IGRAs). The aim of this study was to elucidate the effect of prolonged incubation on the results of the QuantiFERON TB Gold in-tube (QFT-GIT) assay. Patients aged &gt;20 years with suspected tuberculosis (TB) were recruited prospectively from 1 May 2009 to 31 December 2010. In addition, healthy volunteers with no history of TB treatment were included as controls. For each participant, three sets of the QFT-GIT assay were performed using 24-, 48-, and 72-h incubation tests, and the results were compared. Thirty-seven patients with suspected pulmonary TB and 33 healthy controls were enrolled in the study. Of the 37 patients with suspected TB, the QFT-GIT assay results were positive for 28 (75.7%) after a 24-h incubation period. After prolonged incubation, the results differed in four (10.8%) of the 37 patients suspected of having TB. Among 27 patients with culture-confirmed TB, the sensitivities of the QFT-GIT assay after the 24-, 48-, and 72-h incubation tests were 85.2%, 81.5%, and 81.5%, respectively. Among the 33 healthy controls, the QFT-GIT assay results were positive in two (6.1%) after a 24-h incubation period. The results changed for two (6.1%) of the 33 healthy controls after prolonged incubation. The specificities of the QFT-GIT assay after 24, 48, and 72 h of incubation were 93.9%, 87.9%, and 90.9%, respectively. Prolonging the incubation time did not increase the sensitivity of the QFT-GIT assay. The manufacturer-recommended incubation time of 16 to 24 h should be respected because prolonged incubation can cause indeterminate or false-positive results.<\/jats:p>","DOI":"10.1128\/cvi.00290-13","type":"journal-article","created":{"date-parts":[[2013,7,4]],"date-time":"2013-07-04T07:04:01Z","timestamp":1372921441000},"page":"1377-1380","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":8,"title":["Effect of Prolonged Incubation Time on Results of the QuantiFERON TB Gold In-Tube Assay for Diagnosis of Latent Tuberculosis Infection"],"prefix":"10.1128","volume":"20","author":[{"given":"Joo-Won","family":"Min","sequence":"first","affiliation":[{"name":"Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, Kwandong University College of Medicine, Seoul, South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ha-Youn","family":"Lee","sequence":"additional","affiliation":[{"name":"Division of Pulmonology and Critical Care Medicine, Department of Internal Medicine and Lung Institute, Seoul National University College of Medicine, Gwanak, Seoul, South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ji Sun","family":"Lee","sequence":"additional","affiliation":[{"name":"Division of Pulmonology and Critical Care Medicine, Department of Internal Medicine and Lung Institute, Seoul National University College of Medicine, Gwanak, Seoul, South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jinwoo","family":"Lee","sequence":"additional","affiliation":[{"name":"Division of Pulmonology and Critical Care Medicine, Department of Internal Medicine and Lung Institute, Seoul National University College of Medicine, Gwanak, Seoul, South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jae Ho","family":"Chung","sequence":"additional","affiliation":[{"name":"Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, Kwandong University College of Medicine, Seoul, South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sung Koo","family":"Han","sequence":"additional","affiliation":[{"name":"Division of Pulmonology and Critical Care Medicine, Department of Internal Medicine and Lung Institute, Seoul National University College of Medicine, Gwanak, Seoul, South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jae-Joon","family":"Yim","sequence":"additional","affiliation":[{"name":"Division of Pulmonology and Critical Care Medicine, Department of Internal Medicine and Lung Institute, Seoul National University College of Medicine, Gwanak, Seoul, South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"560","DOI":"10.1055\/s-0028-1085707","article-title":"New diagnostics for latent and active tuberculosis: state of the art and future prospects","volume":"29","author":"Pai M","year":"2008","unstructured":"PaiMO'BrienR. 2008. New diagnostics for latent and active tuberculosis: state of the art and future prospects. Semin. Respir. Crit. Care Med. 29:560\u2013568.","journal-title":"Semin. Respir. Crit. Care Med."},{"key":"e_1_3_2_3_2","doi-asserted-by":"crossref","first-page":"2756","DOI":"10.1001\/jama.293.22.2756","article-title":"Discrepancy between the tuberculin skin test and the whole-blood interferon gamma assay for the diagnosis of latent tuberculosis infection in an intermediate tuberculosis-burden country","volume":"293","author":"Kang YA","year":"2005","unstructured":"KangYALeeHWYoonHIChoBHanSKShimYSYimJJ. 2005. Discrepancy between the tuberculin skin test and the whole-blood interferon gamma assay for the diagnosis of latent tuberculosis infection in an intermediate tuberculosis-burden country. JAMA 293:2756\u20132761.","journal-title":"JAMA"},{"key":"e_1_3_2_4_2","doi-asserted-by":"crossref","first-page":"177","DOI":"10.7326\/0003-4819-149-3-200808050-00241","article-title":"Systematic review: T-cell-based assays for the diagnosis of latent tuberculosis infection: an update","volume":"149","author":"Pai M","year":"2008","unstructured":"PaiMZwerlingAMenziesD. 2008. Systematic review: T-cell-based assays for the diagnosis of latent tuberculosis infection: an update. Ann. Intern. Med. 149:177\u2013184.","journal-title":"Ann. Intern. Med."},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01136-11"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00482-10"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00051-11"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00132-07"},{"key":"e_1_3_2_9_2","first-page":"828","article-title":"Intra-assay reliability and robustness of QuantiFERON(R)-TB Gold in-tube test in Zambia","volume":"14","author":"Shanaube K","year":"2010","unstructured":"ShanaubeKDe HaasPSchaapAMoyoMKosloffBDevendraARabyEGodfrey-FaussettPAylesH. 2010. Intra-assay reliability and robustness of QuantiFERON(R)-TB Gold in-tube test in Zambia. Int. J. Tuberc. Lung Dis. 14:828\u2013833.","journal-title":"Int. J. Tuberc. Lung Dis."},{"key":"e_1_3_2_10_2","unstructured":"World Health Organization. 2011. Global tuberculosis control: WHO report 2011. World Health Organization Geneva Switzerland. http:\/\/whqlibdoc.who.int\/publications\/2011\/9789241564380_eng.pdf."}],"container-title":["Clinical and Vaccine Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/CVI.00290-13","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/CVI.00290-13","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,7,29]],"date-time":"2021-07-29T20:49:30Z","timestamp":1627591770000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/CVI.00290-13"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,9]]},"references-count":9,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2013,9]]}},"alternative-id":["10.1128\/CVI.00290-13"],"URL":"https:\/\/doi.org\/10.1128\/cvi.00290-13","relation":{},"ISSN":["1556-6811","1556-679X"],"issn-type":[{"value":"1556-6811","type":"print"},{"value":"1556-679X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,9]]},"assertion":[{"value":"2013-05-03","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-06-13","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-08-26","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}