{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T07:26:45Z","timestamp":1778657205814,"version":"3.51.4"},"reference-count":41,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2006,12,1]],"date-time":"2006-12-01T00:00:00Z","timestamp":1164931200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Vaccine Immunol"],"published-print":{"date-parts":[[2006,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p><jats:italic>Mycobacterium bovis<\/jats:italic>is the causative agent of bovine tuberculosis, a disease that is increasing in incidence in United Kingdom cattle herds. In addition to increasing economic losses, the rise in bovine tuberculosis poses a human health risk. There is an urgent requirement for effective strategies for disease eradication; this will likely involve vaccination in conjunction with current test and slaughter policies. A policy involving vaccination would require an accurate diagnosis of<jats:italic>M. bovis<\/jats:italic>-infected animals and the potential to distinguish these animals from vaccinates. Currently used diagnostic tests, the skin test and gamma interferon (IFN-\u03b3) blood test, have a sensitivity of up to 95%. A further complication is that<jats:italic>M. bovis<\/jats:italic>BCG-vaccinated animals are also scored positive by these tests. We tested the hypothesis that the quantification of IFN-\u03b3-producing lymphocytes by flow cytometric analysis of intracellular IFN-\u03b3 expression would provide a more accurate discrimination of<jats:italic>M. bovis<\/jats:italic>-infected animals from BCG vaccinates. Significant numbers of IFN-\u03b3-expressing CD4<jats:sup>+<\/jats:sup>T cells were detected following culture of heparinized blood from<jats:italic>M. bovis<\/jats:italic>-infected animals, but not from BCG vaccinates, with purified protein derived from<jats:italic>M. bovis<\/jats:italic>(PPD-B) or live mycobacteria. Only 1 of 17 BCG-vaccinated animals had a significant number of CD4<jats:sup>+<\/jats:sup>T lymphocytes expressing IFN-\u03b3, compared with 21\/22<jats:italic>M. bovis<\/jats:italic>-infected animals. This assay could allow an accurate diagnosis of<jats:italic>M. bovis<\/jats:italic>and allow the discrimination of BCG-vaccinated cattle from infected cattle.<\/jats:p>","DOI":"10.1128\/cvi.00291-06","type":"journal-article","created":{"date-parts":[[2006,12,6]],"date-time":"2006-12-06T18:38:08Z","timestamp":1165430288000},"page":"1343-1348","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":14,"title":["Flow Cytometric Detection of Gamma Interferon Can Effectively Discriminate<i>Mycobacterium bovis<\/i>BCG-Vaccinated Cattle from<i>M. bovis<\/i>-Infected Cattle"],"prefix":"10.1128","volume":"13","author":[{"given":"P.","family":"Sopp","sequence":"first","affiliation":[{"name":"Institute for Animal Health, Compton, Newbury, Berkshire RG207NN, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C. 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