{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T03:41:48Z","timestamp":1790912508024,"version":"4.1.0"},"reference-count":26,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2010,12,1]],"date-time":"2010-12-01T00:00:00Z","timestamp":1291161600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Vaccine Immunol"],"published-print":{"date-parts":[[2010,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            T-cell-based gamma interferon (IFN-\u03b3) release assays (IGRAs) using\n            <jats:italic>Mycobacterium tuberculosis<\/jats:italic>\n            -specific antigens have shown higher sensitivity and specificity than the routine tuberculin skin test (TST). However, the effects of\n            <jats:italic>Mycobacterium bovis<\/jats:italic>\n            BCG vaccination and anti-tuberculosis (TB) treatment on dynamic T-cell responses to\n            <jats:italic>M. tuberculosis<\/jats:italic>\n            -specific antigens in active TB cases have rarely been investigated in regions where TB is endemic. Eighty-nine patients with active pulmonary TB (ATB) and 57 healthy controls (HC) from China were recruited and tested by sputum smear and culture, TSTs, and IGRAs with\n            <jats:italic>M. tuberculosis<\/jats:italic>\n            -specific antigens ESAT-6 and CFP-10 (T-SPOT.TB) as well as purified protein derivative (PPD) stimulation. All 146 participants were screened by the T-SPOT.TB assay at recruitment. T-SPOT.TB-positive rates in ATB and HC groups were 87.6% (78\/89) and 21.1% (12\/57), respectively. Of 38 ATB patients who were both TST and T-SPOT.TB tested, the positive rates were 73.7% (28\/38) and 94.7% (36\/38), respectively (\n            <jats:italic>P<\/jats:italic>\n            = 0.0215), and those in the HC group were 62.3% (33\/53) and 18.9% (10\/53), respectively (\n            <jats:italic>P<\/jats:italic>\n            &lt; 0.0001). The T-SPOT.TB-positive rates declined during TB treatment and were 94.4% (51\/54), 86.4% (19\/22), and 61.5% (8\/13) for ATB patients receiving 0- to 1-month, 1- to 3-month, and 3- to 6-month anti-TB treatment, respectively. The IGRA is a most promising test for both active TB and latent TB infection (LTBI) diagnosis due to the improvement of its specificity and convenience, especially in the\n            <jats:italic>Mycobacterium bovis<\/jats:italic>\n            BCG-vaccinated population. Furthermore, the T-SPOT.TB assay using ESAT-6 and CFP-10 in ATB patients during anti-TB treatment could serve as a potential predictor of therapeutic efficacy.\n          <\/jats:p>","DOI":"10.1128\/cvi.00294-10","type":"journal-article","created":{"date-parts":[[2010,10,14]],"date-time":"2010-10-14T07:13:46Z","timestamp":1287040426000},"page":"1985-1990","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":41,"title":["Evaluation of Gamma Interferon Release Assays Using\n            <i>Mycobacterium tuberculosis<\/i>\n            Antigens for Diagnosis of Latent and Active Tuberculosis in\n            <i>Mycobacterium bovis<\/i>\n            BCG-Vaccinated Populations"],"prefix":"10.1128","volume":"17","author":[{"given":"Shu","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Infectious Diseases, Huashan Hospital, Fudan University, Shanghai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lingyun","family":"Shao","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Huashan Hospital, Fudan University, Shanghai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ling","family":"Mo","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Huashan Hospital, Fudan University, Shanghai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiazhen","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Huashan Hospital, Fudan University, Shanghai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feifei","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Huashan Hospital, Fudan University, Shanghai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chengyan","family":"Meng","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Huashan Hospital, Fudan University, Shanghai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min","family":"Zhong","sequence":"additional","affiliation":[{"name":"Chongqing Pulmonary Hospital, Chongqing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lihua","family":"Qiu","sequence":"additional","affiliation":[{"name":"Department of Tuberculosis, Shandong Chest Hospital, Jinan, Shandong Province, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meiying","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Tuberculosis Infection, Suzhou No. 5 People's Hospital, Suzhou, Jiangsu Province, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinhua","family":"Weng","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Huashan Hospital, Fudan University, Shanghai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenhong","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Huashan Hospital, Fudan University, Shanghai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"1099","DOI":"10.1016\/S0140-6736(00)02742-2","volume":"356","year":"2000","unstructured":"Andersen, P., M. 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