{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T01:46:16Z","timestamp":1791942376898,"version":"4.3.4"},"reference-count":37,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2012,9,1]],"date-time":"2012-09-01T00:00:00Z","timestamp":1346457600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Vaccine Immunol"],"published-print":{"date-parts":[[2012,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Recently, novel\n            <jats:named-content content-type=\"genus-species\">Brucella<\/jats:named-content>\n            strains with phenotypic characteristics that were atypical for strains belonging to the genus\n            <jats:named-content content-type=\"genus-species\">Brucella<\/jats:named-content>\n            have been reported. Phenotypically many of these strains were initially misidentified as\n            <jats:named-content content-type=\"genus-species\">Ochrobactrum<\/jats:named-content>\n            spp. Two novel species have been described so far for these strains, i.e.,\n            <jats:named-content content-type=\"genus-species\">B. microti<\/jats:named-content>\n            and\n            <jats:named-content content-type=\"genus-species\">B. inopinata<\/jats:named-content>\n            , and other strains genetically related to\n            <jats:named-content content-type=\"genus-species\">B. inopinata<\/jats:named-content>\n            may constitute other novel species as well. In this study, we analyzed the lipopolysaccharides (LPS) (smooth LPS [S-LPS] and rough LPS [R-LPS]) of these atypical strains using different methods and a panel of monoclonal antibodies (MAbs) directed against several epitopes of the\n            <jats:named-content content-type=\"genus-species\">Brucella<\/jats:named-content>\n            O-polysaccharide (O-PS) and R-LPS. Among the most striking results,\n            <jats:named-content content-type=\"genus-species\">Brucella<\/jats:named-content>\n            sp. strain BO2, isolated from a patient with chronic destructive pneumonia, showed a completely distinct S-LPS profile in silver stain gels that looked more similar to that of enterobacterial S-LPS. This strain also failed to react with MAbs against\n            <jats:named-content content-type=\"genus-species\">Brucella<\/jats:named-content>\n            O-PS epitopes and showed weak reactivity with anti-R-LPS MAbs.\n            <jats:named-content content-type=\"genus-species\">B. inopinata<\/jats:named-content>\n            reference strain BO1 displayed an M-dominant S-LPS type with some heterogeneity relative to the classical M-dominant\n            <jats:named-content content-type=\"genus-species\">Brucella<\/jats:named-content>\n            S-LPS type. Australian wild rodent strains belonging also to the\n            <jats:named-content content-type=\"genus-species\">B. inopinata<\/jats:named-content>\n            group showed a classical A-dominant S-LPS but lacked the O-PS common (C) epitopes, as previously reported for\n            <jats:named-content content-type=\"genus-species\">B. suis<\/jats:named-content>\n            biovar 2 strains. Interestingly, some strains also failed to react with anti-R-LPS MAbs, such as the\n            <jats:named-content content-type=\"genus-species\">B. microti<\/jats:named-content>\n            reference strain and\n            <jats:named-content content-type=\"genus-species\">B. inopinata<\/jats:named-content>\n            BO1, suggesting modifications in the core-lipid A moieties of these strains. These results have several implications for serological typing and serological diagnosis and underline the need for novel tools for detection and correct identification of such novel emerging\n            <jats:named-content content-type=\"genus-species\">Brucella<\/jats:named-content>\n            spp.\n          <\/jats:p>","DOI":"10.1128\/cvi.00300-12","type":"journal-article","created":{"date-parts":[[2012,7,4]],"date-time":"2012-07-04T21:09:14Z","timestamp":1341436154000},"page":"1370-1373","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":35,"title":["Lipopolysaccharide Heterogeneity in the Atypical Group of Novel Emerging Brucella Species"],"prefix":"10.1128","volume":"19","author":[{"given":"Michel S.","family":"Zygmunt","sequence":"first","affiliation":[{"name":"INRA, UMR1282 Infectiologie et Sant\u00e9 Publique, Nouzilly, France"},{"name":"Universit\u00e9 Fran\u00e7ois Rabelais de Tours, UMR1282 Infectiologie et Sant\u00e9 Publique, Tours, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabelle","family":"Jacques","sequence":"additional","affiliation":[{"name":"INRA, UMR1282 Infectiologie et Sant\u00e9 Publique, Nouzilly, France"},{"name":"Universit\u00e9 Fran\u00e7ois Rabelais de Tours, UMR1282 Infectiologie et Sant\u00e9 Publique, Tours, France"},{"name":"IUT de Tours, Tours, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nelly","family":"Bernardet","sequence":"additional","affiliation":[{"name":"INRA, UMR1282 Infectiologie et Sant\u00e9 Publique, Nouzilly, France"},{"name":"Universit\u00e9 Fran\u00e7ois Rabelais de Tours, UMR1282 Infectiologie et Sant\u00e9 Publique, Tours, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Axel","family":"Cloeckaert","sequence":"additional","affiliation":[{"name":"INRA, UMR1282 Infectiologie et Sant\u00e9 Publique, Nouzilly, France"},{"name":"Universit\u00e9 Fran\u00e7ois Rabelais de Tours, UMR1282 Infectiologie et Sant\u00e9 Publique, Tours, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","volume-title":"Techniques for the brucellosis laboratory","author":"Alton GG","year":"1988","unstructured":"AltonGG JonesLM AngusRD VergerJM. 1988. 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