{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,3]],"date-time":"2026-11-03T01:08:59Z","timestamp":1793668139706,"version":"4.5.1"},"reference-count":44,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2017,1,1]],"date-time":"2017-01-01T00:00:00Z","timestamp":1483228800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["HD068229"],"award-info":[{"award-number":["HD068229"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["HD044864"],"award-info":[{"award-number":["HD044864"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["HD079918"],"award-info":[{"award-number":["HD079918"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["HD082273"],"award-info":[{"award-number":["HD082273"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["AI114013"],"award-info":[{"award-number":["AI114013"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Vaccine Immunol"],"published-print":{"date-parts":[[2017,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Subunit vaccines for prevention of congenital cytomegalovirus (CMV) infection based on glycoprotein B (gB) and pp65 are in clinical trials, but it is unclear whether simultaneous vaccination with both antigens enhances protection. We undertook evaluation of a novel bivalent vaccine based on nonreplicating lymphocytic choriomeningitis virus (rLCMV) vectors expressing a cytoplasmic tail-deleted gB [gB(dCt)] and full-length pp65 from human CMV in mice. Immunization with the gB(dCt) vector alone elicited a comparable gB-binding antibody response and a superior neutralizing response to that elicited by adjuvanted subunit gB. Immunization with the pp65 vector alone elicited robust T cell responses. Comparable immunogenicity of the combined gB(dCt) and pp65 vectors with the individual monovalent formulations was demonstrated. To demonstrate proof of principle for a bivalent rLCMV-based HCMV vaccine, the congenital guinea pig cytomegalovirus (GPCMV) infection model was used to compare rLCMV vectors encoding homologs of pp65 (GP83) and gB(dCt), alone and in combination versus Freund's adjuvanted recombinant gB. Both vectors elicited significant immune responses, and no loss of gB immunogenicity was noted with the bivalent formulation. Combined vaccination with rLCMV-vectored GPCMV gB(dCt) and pp65 (GP83) conferred better protection against maternal viremia than subunit or either monovalent rLCMV vaccine. The bivalent vaccine also was significantly more effective in reducing pup mortality than the monovalent vaccines. In summary, bivalent vaccines with rLCMV vectors expressing gB and pp65 elicited potent humoral and cellular responses and conferred protection in the GPCMV model. Further clinical trials of LCMV-vectored HCMV vaccines are warranted.<\/jats:p>","DOI":"10.1128\/cvi.00300-16","type":"journal-article","created":{"date-parts":[[2016,10,26]],"date-time":"2016-10-26T23:00:19Z","timestamp":1477522819000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":38,"title":["Additive Protection against Congenital Cytomegalovirus Conferred by Combined Glycoprotein B\/pp65 Vaccination Using a Lymphocytic Choriomeningitis Virus Vector"],"prefix":"10.1128","volume":"24","author":[{"given":"Mark R.","family":"Schleiss","sequence":"first","affiliation":[{"name":"Center for Infectious Diseases and Microbiology Translational Research and Department of Pediatrics, University of Minnesota Medical School, Minneapolis, Minnesota, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ursula","family":"Berka","sequence":"additional","affiliation":[{"name":"Hookipa Biotech AG, Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth","family":"Watson","sequence":"additional","affiliation":[{"name":"Hookipa Biotech AG, Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mario","family":"Aistleithner","sequence":"additional","affiliation":[{"name":"Hookipa Biotech AG, Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bettina","family":"Kiefmann","sequence":"additional","affiliation":[{"name":"Hookipa Biotech AG, Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bastien","family":"Mangeat","sequence":"additional","affiliation":[{"name":"Department of Pathology and Immunology, University of Geneva, Geneva, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth C.","family":"Swanson","sequence":"additional","affiliation":[{"name":"Center for Infectious Diseases and Microbiology Translational Research and Department of Pediatrics, University of Minnesota Medical School, Minneapolis, Minnesota, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter A.","family":"Gillis","sequence":"additional","affiliation":[{"name":"Center for Infectious Diseases and Microbiology Translational Research and Department of Pediatrics, University of Minnesota Medical School, Minneapolis, Minnesota, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nelmary","family":"Hernandez-Alvarado","sequence":"additional","affiliation":[{"name":"Center for Infectious Diseases and Microbiology Translational Research and Department of Pediatrics, University of Minnesota Medical School, Minneapolis, Minnesota, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudia","family":"Fern\u00e1ndez-Alarc\u00f3n","sequence":"additional","affiliation":[{"name":"Center for Infectious Diseases and Microbiology Translational Research and Department of Pediatrics, University of Minnesota Medical School, Minneapolis, Minnesota, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jason C.","family":"Zabeli","sequence":"additional","affiliation":[{"name":"Center for Infectious Diseases and Microbiology Translational Research and Department of Pediatrics, University of Minnesota Medical School, Minneapolis, Minnesota, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel D.","family":"Pinschewer","sequence":"additional","affiliation":[{"name":"Department of Pathology and Immunology, University of Geneva, Geneva, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anders E.","family":"Lilja","sequence":"additional","affiliation":[{"name":"Hookipa Biotech AG, Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Schwendinger","sequence":"additional","affiliation":[{"name":"Hookipa Biotech AG, Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Farshad","family":"Guirakhoo","sequence":"additional","affiliation":[{"name":"Hookipa Biotech AG, Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas P.","family":"Monath","sequence":"additional","affiliation":[{"name":"Hookipa Biotech AG, Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Klaus K.","family":"Orlinger","sequence":"additional","affiliation":[{"name":"Hookipa Biotech AG, Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/path.4437"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI45449"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1086\/421999"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molmed.2005.11.006"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1586\/erv.10.125"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2014.03.057"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cit587"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20050882"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa0804749"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2015.11.056"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(11)60136-0"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(11)70344-9"},{"key":"e_1_3_3_14_2","doi-asserted-by":"crossref","first-page":"1080","DOI":"10.1016\/0264-410X(95)00048-6","article-title":"Preclinical evaluation of an ALVAC (canarypox)-human cytomegalovirus glycoprotein B vaccine candidate","volume":"13","author":"G\u00f6ncz\u00f6l E","year":"1995","unstructured":"G\u00f6ncz\u00f6l E BerensciK PincusS EndreszV M\u00e9ricC PaolettiE PlotkinSA. 1995. Preclinical evaluation of an ALVAC (canarypox)-human cytomegalovirus glycoprotein B vaccine candidate. Vaccine13:1080\u20131085.","journal-title":"Vaccine"},{"key":"e_1_3_3_15_2","doi-asserted-by":"crossref","first-page":"1171","DOI":"10.1086\/319680","article-title":"A canarypox vector-expressing cytomegalovirus (CMV) phosphoprotein 65 induces long-lasting cytotoxic T cell responses in human CMV-seronegative subjects","volume":"183","author":"Berencsi K","year":"2001","unstructured":"Berencsi K GyulaiZ G\u00f6ncz\u00f6lE PincusS CoxWI MichelsonS KariL MericC CadozM ZahradnikJ StarrS PlotkinS. 2001. A canarypox vector-expressing cytomegalovirus (CMV) phosphoprotein 65 induces long-lasting cytotoxic T cell responses in human CMV-seronegative subjects. J Infect Dis183:1171\u20131179.","journal-title":"J Infect Dis"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2009.09.135"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1004524"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/5.2.177"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2007.10.008"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.2217\/fvi.13.016"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1086\/511982"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2015.06.019"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0600652103"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm.2104"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.72.9.7374-7386.1998"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01718317"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.73.10.8677-8688.1999"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(88)90071-2"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(84)90275-7"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1586\/14760584.2013.811188"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.62.9.3309-3318.1988"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.4161\/hv.1.1.1335"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2014.05.011"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pcl.2012.12.008"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11908-014-0419-8"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/S2352-3026(15)00286-0"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1086\/649903"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2016.03.005"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2013.04.015"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2008.07.092"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.05128-11"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2011.01.079"},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.4161\/hv.22447"},{"key":"e_1_3_3_44_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01419-15"},{"key":"e_1_3_3_45_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jim.2007.08.012"}],"container-title":["Clinical and Vaccine Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/CVI.00300-16","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/CVI.00300-16","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,7,29]],"date-time":"2021-07-29T11:43:02Z","timestamp":1627558982000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/CVI.00300-16"}},"subtitle":[],"editor":[{"given":"Kathryn M.","family":"Edwards","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2017,1]]},"references-count":44,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2017,1]]}},"alternative-id":["10.1128\/CVI.00300-16"],"URL":"https:\/\/doi.org\/10.1128\/cvi.00300-16","relation":{"has-review":[{"id-type":"doi","id":"10.3410\/f.726944913.793527224","asserted-by":"object"}]},"ISSN":["1556-6811","1556-679X"],"issn-type":[{"value":"1556-6811","type":"print"},{"value":"1556-679X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,1]]},"assertion":[{"value":"2016-06-20","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-10-10","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-01-05","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}