{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,14]],"date-time":"2026-01-14T18:03:37Z","timestamp":1768413817760,"version":"3.49.0"},"reference-count":45,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2011,1,1]],"date-time":"2011-01-01T00:00:00Z","timestamp":1293840000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Vaccine Immunol"],"published-print":{"date-parts":[[2011,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p><jats:italic>Haemophilus parasuis<\/jats:italic>is the agent responsible for causing Gla\u0308sser's disease, which is characterized by fibrinous polyserositis, polyarthritis, and meningitis in pigs. In this study, we have characterized native outer membrane proteins with affinity to porcine transferrin (NPAPT) from<jats:italic>H. parasuis<\/jats:italic>serovar 5, Nagasaki strain. This pool of proteins was used as antigen to developed two vaccine formulations: one was adjuvanted with a mineral oil (Montanide IMS 2215 VG PR), while the other was potentiated with a bacterial neuraminidase from<jats:italic>Clostridium perfringens<\/jats:italic>. The potential protective effect conferred by these two vaccines was compared to that afforded by two other vaccines, consisting of recombinant transferrin-binding protein (rTbp) A or B fragments from<jats:italic>H. parasuis<\/jats:italic>, Nagasaki strain, and by a commercially available inactivated vaccine. Five groups of colostrum-deprived piglets immunized with the vaccines described above, one group per each vaccine, and a group of nonvaccinated control animals were challenged intratracheally with a lethal dose (3 \u00d7 10<jats:sup>8<\/jats:sup>CFU) of<jats:italic>H. parasuis<\/jats:italic>, Nagasaki strain. The two vaccines containing rTbps yielded similar results with minimal protection against death, clinical signs, gross and microscopic lesions, and<jats:italic>H. parasuis<\/jats:italic>invasion. In contrast, the two vaccines composed of NPAPT antigen and commercial bacterin resulted in a strong protection against challenge (without deaths and clinical signs), mild histopathological changes, and no recovery of<jats:italic>H. parasuis<\/jats:italic>, thus suggesting their effectiveness in preventing Gla\u0308sser's disease outbreaks caused by serovar 5.<\/jats:p>","DOI":"10.1128\/cvi.00314-10","type":"journal-article","created":{"date-parts":[[2010,10,7]],"date-time":"2010-10-07T02:40:05Z","timestamp":1286419205000},"page":"50-58","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":41,"title":["Development and Characterization of Protective<i>Haemophilus parasuis<\/i>Subunit Vaccines Based on Native Proteins with Affinity to Porcine Transferrin and Comparison with Other Subunit and Commercial Vaccines"],"prefix":"10.1128","volume":"18","author":[{"given":"Rafael","family":"Frandoloso","sequence":"first","affiliation":[{"name":"Section of Microbiology and Immunology, Department of Animal Health, University of Leo\u0301n, Leo\u0301n, Spain"},{"name":"Section of Histology and Pathological Anatomy, Department of Animal Health, University of Leo\u0301n, Leo\u0301n, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sonia","family":"Marti\u0301nez","sequence":"additional","affiliation":[{"name":"Section of Microbiology and Immunology, Department of Animal Health, University of Leo\u0301n, Leo\u0301n, Spain"},{"name":"Section of Histology and Pathological Anatomy, Department of Animal Health, University of Leo\u0301n, Leo\u0301n, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eli\u0301as F.","family":"Rodri\u0301guez-Ferri","sequence":"additional","affiliation":[{"name":"Section of Microbiology and Immunology, Department of Animal Health, University of Leo\u0301n, Leo\u0301n, Spain"},{"name":"Section of Histology and Pathological Anatomy, Department of Animal Health, University of Leo\u0301n, Leo\u0301n, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mari\u0301a Jose\u0301","family":"Garci\u0301a-Iglesias","sequence":"additional","affiliation":[{"name":"Section of Microbiology and Immunology, Department of Animal Health, University of Leo\u0301n, Leo\u0301n, Spain"},{"name":"Section of Histology and Pathological Anatomy, Department of Animal Health, University of Leo\u0301n, Leo\u0301n, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claudia","family":"Pe\u0301rez-Marti\u0301nez","sequence":"additional","affiliation":[{"name":"Section of Microbiology and Immunology, Department of Animal Health, University of Leo\u0301n, Leo\u0301n, Spain"},{"name":"Section of Histology and Pathological Anatomy, Department of Animal Health, University of Leo\u0301n, Leo\u0301n, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Beatriz","family":"Marti\u0301nez-Ferna\u0301ndez","sequence":"additional","affiliation":[{"name":"Section of Microbiology and Immunology, Department of Animal Health, University of Leo\u0301n, Leo\u0301n, Spain"},{"name":"Section of Histology and Pathological Anatomy, Department of Animal Health, University of Leo\u0301n, Leo\u0301n, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ce\u0301sar B.","family":"Gutie\u0301rrez-Marti\u0301n","sequence":"additional","affiliation":[{"name":"Section of Microbiology and Immunology, Department of Animal Health, University of Leo\u0301n, Leo\u0301n, Spain"},{"name":"Section of Histology and Pathological Anatomy, Department of Animal Health, University of Leo\u0301n, Leo\u0301n, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"542","DOI":"10.1016\/j.vaccine.2008.11.020","volume":"27","year":"2009","unstructured":"Altindis, E., B. E. Tefon, V. Yildirim, E. Ozcengiz, D. Becher, M. Hecker, and G. Ozcengiz. 2009. Immunoproteomic analysis of Bordetella pertussis and identification of new immunogenic proteins. Vaccine 27:542-548.","journal-title":"Vaccine"},{"key":"e_1_3_2_3_2","doi-asserted-by":"crossref","first-page":"639","DOI":"10.1292\/jvms.56.639","volume":"56","year":"1994","unstructured":"Amano, H., M. Shibata, N. Kajio, and T. Morozumi. 1994. Pathologic observations of pigs intranasally inoculated with serovars 1, 4, and 5 of Haemophilus parasuis using immunoperoxidase method. J. Vet. Med. Sci. 56:639-644.","journal-title":"J. Vet. Med. Sci."},{"key":"e_1_3_2_4_2","doi-asserted-by":"crossref","first-page":"451","DOI":"10.1292\/jvms.59.451","volume":"59","year":"1997","unstructured":"Amano, H., M. Shibata, K. Takahashi, and Y. Sasaki, Y. 1997. Effects on endotoxin pathogenicity in pigs with acute septicemia of Haemophilus parasuis infection. J. Vet. Med. Sci. 59:451-455.","journal-title":"J. Vet. Med. Sci."},{"key":"e_1_3_2_5_2","doi-asserted-by":"crossref","first-page":"266","DOI":"10.1016\/j.vetmic.2006.10.008","volume":"119","year":"2007","unstructured":"Angen, O., S. Oliveira, P. Ahrens, B. Svensmark, and T. D. Leser. 2007. Development of an improved species specific PCR test for detection of Haemophilus parasuis. Vet. Microbiol. 119:266-276.","journal-title":"Vet. Microbiol."},{"key":"e_1_3_2_6_2","doi-asserted-by":"crossref","first-page":"502","DOI":"10.1136\/vr.151.17.502","volume":"151","year":"2002","unstructured":"Bak, H., and H. J. Riising. 2002. Protection of vaccinated pigs against experimental infections with homologous and heterologous Haemophilus parasuis. Vet. Rec. 151:502-505.","journal-title":"Vet. Rec."},{"key":"e_1_3_2_7_2","first-page":"228","volume":"72","year":"2008","unstructured":"Blanco, I., A. Canals, G. Evans, M. A. Mellencamp, C. Cia, N. Deeb, L. Wang, and L. Galina-Pantoja. 2008. Differences in susceptibility to Haemophilus parasuis infection in pigs. Can. J. Vet. Res. 72:228-235.","journal-title":"Can. J. Vet. Res."},{"key":"e_1_3_2_8_2","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/j.vetmic.2004.06.011","volume":"103","year":"2004","unstructured":"Blanco, I., L. Galina-Pantoja, S. Oliveira, C. Pijoan, C. S\u00e1nchez, and A. Canals. 2004. Comparison between Haemophilus parasuis infection in colostrums deprived and sow-reared piglets. Vet. Microbiol. 103:21-27.","journal-title":"Vet. Microbiol."},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.2.880-882.2003"},{"key":"e_1_3_2_10_2","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/j.femsim.2005.02.008","volume":"45","year":"2005","unstructured":"del R\u00edo, M. L., C. B. Guti\u00e9rrez-Mart\u00edn, J. I. Rodr\u00edguez Barbosa, J. Navas, and E. F. Rodr\u00edguez Ferri. 2005. Identification and characterization of the TonB region and its role in transferrin-mediated iron acquisition in Haemophilus parasuis. FEMS Immunol. Med. Microbiol. 45:75-86.","journal-title":"FEMS Immunol. Med. Microbiol."},{"key":"e_1_3_2_11_2","first-page":"966","volume":"19","year":"2001","unstructured":"Goethe, R., O. Flores Gonz\u00e1les, T. Lindner, and G. F. Gerlach. 2001. A novel strategy for protective Actinobacillus pleuropneumoniae subunit vaccines: detergent extraction of cultures induced by iron restriction. Vaccine 19:966-975.","journal-title":"Vaccine"},{"key":"e_1_3_2_12_2","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1016\/0966-842X(96)10025-1","volume":"4","year":"1996","unstructured":"Gray-Owen, S. D., and A. B. Schryvers. 1996. Bacterial transferrin and lactoferrin receptors. Trends Microbiol. 4:185-191.","journal-title":"Trends Microbiol."},{"key":"e_1_3_2_13_2","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1016\/S0378-1135(03)00212-8","volume":"96","year":"2003","unstructured":"Hill, C. E., D. S. Metcalf, and J. I. MacInnes. 2003. A search for virulence genes of Haemophilus parasuis using differential display RT-PCR. Vet. Microbiol. 96:189-202.","journal-title":"Vet. Microbiol."},{"key":"e_1_3_2_14_2","first-page":"271","volume":"109","year":"2002","unstructured":"Hoffmann, C. R., and G. Bilkei. 2002. The effect of a homologous bacterin given to sows prefarrowing on the development of Gl\u00e4sser's disease in postweaning pigs after i.v. challenge with Haemophilus parasuis serotype 5. Deut. Tier\u00e4rztl. Woch. 109:271-276.","journal-title":"Deut. Tier\u00e4rztl. Woch."},{"key":"e_1_3_2_15_2","first-page":"191","volume":"15","year":"2000","unstructured":"Jarosik, G. P. 2000. Binding of catalase by Gardnerella vaginalis. FEMS Microbiol. Lett. 15:191-194.","journal-title":"FEMS Microbiol. Lett."},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.30.4.862-865.1992"},{"key":"e_1_3_2_17_2","doi-asserted-by":"crossref","first-page":"77","DOI":"10.54846\/jshap\/286","volume":"9","year":"2001","unstructured":"Kirkwood, R. N., S. A. Rawluk, A. C. Cegielski, and A. J. Otto. 2001. Effect of pig age and autogenous sow vaccination on nasal mucosal colonization of pigs by Haemophilus parasuis. J. Swine Health Prod. 9:77-79.","journal-title":"J. Swine Health Prod."},{"key":"e_1_3_2_18_2","doi-asserted-by":"crossref","first-page":"754","DOI":"10.1016\/j.bbrc.2008.12.120","volume":"379","year":"2009","unstructured":"Kuroiwa, A., A. Hisatsune, Y. Isohama, and H. Katsuki, H. 2009. Bacterial neuraminidase increases IL-8 production in lung epithelial cells via NF-\u03baB-dependent pathway. Biochem. Biophys. Res. Commun. 379:754-759.","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"e_1_3_2_19_2","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1099\/00222615-34-6-317","volume":"34","year":"1991","unstructured":"Lee, B. C. 1991. Iron sources for Haemophilus ducreyi. J. Med. Microbiol. 34:317-322.","journal-title":"J. Med. Microbiol."},{"key":"e_1_3_2_20_2","doi-asserted-by":"crossref","first-page":"1484","DOI":"10.1074\/mcp.M600111-MCP200","volume":"5","year":"2006","unstructured":"Lin, Y. F., M. S. Wu, C. C. Chang, S. W. Lin, J. T. Lin, Y. J. Sun, D. S. Chen, and L. P. Chow. 2006. Comparative immunoproteomics of identification and characterization of virulence factors from Helicobacter pylori related to gastric cancer. Mol. Cell Proteomics. 5:1484-1496.","journal-title":"Mol. Cell Proteomics."},{"key":"e_1_3_2_21_2","doi-asserted-by":"crossref","first-page":"575","DOI":"10.1046\/j.1365-2958.1996.406943.x","volume":"19","year":"1996","unstructured":"Loosmore, S. M., Y. Yang, D. C. Coleman, J. M. Shortreed, D. M. England, R. E. Harkness, P. S. C. Chong, and M. H. Klein. 1996. Cloning and expression of the Haemophilus influenzae transferrin receptor gene. Mol. Microbiol. 19:575-586.","journal-title":"Mol. Microbiol."},{"key":"e_1_3_2_22_2","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1016\/j.jcpa.2008.10.007","volume":"140","year":"2009","unstructured":"Mart\u00edn de la Fuente, A. J., C. B. Guti\u00e9rrez Mart\u00edn, C. P\u00e9rez Mart\u00ednez, M. J. Garc\u00eda Iglesias, F. Tejerina, and E. F. Rodr\u00edguez Ferri. 2009. Effect of different vaccine formulations on the development of Gl\u00e4sser's disease induced in pigs by experimental Haemophilus parasuis infection. J. Comp. Pathol. 140:169-176.","journal-title":"J. Comp. Pathol."},{"key":"e_1_3_2_23_2","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1111\/j.1574-6968.2010.01970.x","volume":"307","year":"2010","unstructured":"Mart\u00ednez, S., R. Frandoloso, E. F. Rodr\u00edguez-Ferri, B. Gonz\u00e1lez-Z\u00f6rn, and C. B. Guti\u00e9rrez-Mart\u00edn. 2010. Characterization of a recombinant transferrin-binding protein A (TbpA) fragment from Haemophilus parasuis serovar 5. FEMS Microbiol. Lett. 307:142-150.","journal-title":"FEMS Microbiol. Lett."},{"key":"e_1_3_2_24_2","doi-asserted-by":"crossref","first-page":"2651","DOI":"10.1002\/pmic.200701056","volume":"8","year":"2008","unstructured":"Mart\u00ednez L\u00f3pez, R., C. Nombela, R. D\u00edez-Orejas, L. Monteoliva, and C. Gil. 2008. Immunoproteomics analysis of the protective response obtained from vaccination with Candida albicans ecm33 cell wall mutant in mice. Proteomics 8:2651-2664.","journal-title":"Proteomics"},{"key":"e_1_3_2_25_2","first-page":"33","volume":"55","year":"1991","unstructured":"Miniats, O. P., N. L. Smart, and S. Rosendal. 1991. Vaccination of gnotobiotic primary specific pathogen-free pigs against Haemophilus parasuis. Can. J. Vet. Res. 55:33-36.","journal-title":"Can. J. Vet. Res."},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.66.9.4183-4192.1998"},{"key":"e_1_3_2_27_2","first-page":"146","volume":"67","year":"2003","unstructured":"Oliveira, S., L. Galina, I. Blanco, A. Canals, and C. Pijoan. 2003. Naturally farrowed, artificially reared pigs as an alternative model for experimental infection by Haemophilus parasuis. Can. J. Vet. Res. 67:146-150.","journal-title":"Can. J. Vet. Res."},{"key":"e_1_3_2_28_2","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.vetmic.2003.12.001","volume":"99","year":"2004","unstructured":"Oliveira, S., and C. Pijoan. 2004. Haemophilus parasuis: new trends on diagnosis, epidemiology and control. Vet. Microbiol. 99:1-12.","journal-title":"Vet. Microbiol."},{"key":"e_1_3_2_29_2","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1024\/0036-7281.149.9.389","volume":"149","year":"2007","unstructured":"Palzer, A., M. Ritzmann, and K. Hienritzi. 2007. A field trial for early vaccination against Gl\u00e4sser's disease using Porcilis Gl\u00e4sser. Schweiz. Arch. Tierheilkder. 149:389-394.","journal-title":"Schweiz. Arch. Tierheilkder."},{"key":"e_1_3_2_30_2","first-page":"187","volume":"60","year":"1983","unstructured":"Peet, R. L., J. Fry, J. Lloyd, J. Henderson, J. Curran, and D. Moir. 1983. Haemophilus parasuis septicemia in pigs. Aust. J. Vet. Res. 60:187.","journal-title":"Aust. J. Vet. Res."},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00703-08"},{"key":"e_1_3_2_32_2","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1006\/mpat.1999.0297","volume":"27","year":"1999","unstructured":"Potter, A. A., A. B. Schryvers, J. A. Ogunnariwo, W. A. Hutchins, R. T. C. Lo, and T. Watt. 1999. Protective capacity of the Pasteurella haemolytica transferrin-binding proteins TbpA and TbpB in cattle. Microb. Pathog. 27:197-206.","journal-title":"Microb. Pathog."},{"key":"e_1_3_2_33_2","doi-asserted-by":"crossref","first-page":"172","DOI":"10.1111\/j.1751-0813.2000.tb10586.x","volume":"78","year":"2000","unstructured":"Raffie, M., and P. J. Blackall. 2000. Establishment, validation and use of the Kielstein-Rapp-Gabrielson serotyping scheme for Haemophilus parasuis. Aust. Vet. J. 78:172-174.","journal-title":"Aust. Vet. J."},{"key":"e_1_3_2_34_2","first-page":"83","volume":"92","year":"1997","unstructured":"Rapp-Gabrielson, V. J., G. J. Kocur, J. T. Clark, and S. K. Muir. 1997. Haemophilus parasuis: immunity in swine after vaccination. Vet. Med. 92:83-90.","journal-title":"Vet. Med."},{"key":"e_1_3_2_35_2","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1006\/mpat.1998.0226","volume":"25","year":"1998","unstructured":"Retzer, M. D., R. Yu, Y. Zhang, G. C. Gonz\u00e1lez, and A. B. Schryvers. 1998. Discrimination between apo and iron-loaded forms of transferrin by transferrin binding protein B and its N-terminal subfragment. Microb. Pathog. 25:175-180.","journal-title":"Microb. Pathog."},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.65.1.55-63.1997"},{"key":"e_1_3_2_37_2","doi-asserted-by":"crossref","first-page":"512","DOI":"10.1016\/0264-410X(92)90349-O","volume":"10","year":"1992","unstructured":"Rossi-Campos, A., C. Anderson, G. F. Gerlach, S. Klashinsky, A. Potter, and P. J. Willson. 1992. Immunization of pigs against Actinobacillus pleuropneumoniae with two recombinant protein preparations. Vaccine 10:512-518.","journal-title":"Vaccine"},{"key":"e_1_3_2_38_2","doi-asserted-by":"crossref","first-page":"5150","DOI":"10.1021\/ac9806005","volume":"70","year":"1998","unstructured":"Sechi, S., and B. T. Chait. 1998. Modification of cysteine residues by alkylation: a tool in peptide mapping and protein identification. Anal. Chem. 70:5150-5158.","journal-title":"Anal. Chem."},{"key":"e_1_3_2_39_2","first-page":"390","volume":"53","year":"1989","unstructured":"Smart, N. L., and O. P. Miniats. 1989. Preliminary assessment of a Haemophilus parasuis bacterin for use in specific pathogen free swine. Can. J. Vet. Res. 53:390-393.","journal-title":"Can. J. Vet. Res."},{"key":"e_1_3_2_40_2","first-page":"307","volume":"75","year":"2003","unstructured":"Stamatos, N. M., S. Curreli, D. Zella, and A. S. Cross. 2003. Desialylation of glycoconjugates on the surface of monocytes activates the extracellular signal-related kinases ERK1\/2 and results in enhanced production of specific cytokines. J. Leukoc. Biol. 75:307-313.","journal-title":"J. Leukoc. Biol."},{"key":"e_1_3_2_41_2","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1292\/jvms.63.487","volume":"63","year":"2001","unstructured":"Takahaschi, L., S. Nagai, T. Yagihashi, T. Idehata, Y. Nakano, K. Senna, T. Maruyama, and J. Murofushi. 2001. A cross protection experiment in pigs vaccinated with Haemophilus parasuis serovars 2 and 5 bacterins, and evaluation of a bivalent vaccine under laboratory and field conditions. J. Vet. Med. Sci. 63:487-491.","journal-title":"J. Vet. Med. Sci."},{"key":"e_1_3_2_42_2","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1016\/j.vetmic.2004.12.019","volume":"106","year":"2005","unstructured":"Turni, C., and P. J. Blackall. 2005. Comparison of the indirect haemagglutination and gel diffusion test for serotyping Haemophilus parasuis. Vet. Microbiol. 106:145-151.","journal-title":"Vet. Microbiol."},{"key":"e_1_3_2_43_2","doi-asserted-by":"crossref","first-page":"476","DOI":"10.1177\/104063879500700409","volume":"7","year":"1995","unstructured":"Vahle, J. L., J. S. Haynes, and J. J. Andrews. 1995. Experimental reproduction of Haemophilus parasuis infection in swine: clinical, bacteriologic, and morphologic findings. J. Vet. Diagn. Invest. 7:476-480.","journal-title":"J. Vet. Diagn. Invest."},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00701-09"},{"key":"e_1_3_2_45_2","doi-asserted-by":"crossref","first-page":"408","DOI":"10.1016\/j.vetmic.2008.11.019","volume":"136","year":"2009","unstructured":"Zhang, B., C. Tang, F. Yang, and H. Yue. 2009. Molecular cloning, sequencing and expression of the outer membrane protein A gene from Haemophilus parasuis. Vet. Microbiol. 136:408-410.","journal-title":"Vet. Microbiol."},{"key":"e_1_3_2_46_2","doi-asserted-by":"crossref","first-page":"5271","DOI":"10.1016\/j.vaccine.2009.06.051","volume":"27","year":"2009","unstructured":"Zhou, M., Y. Guo, J. Zhao, Q. Hu, Y. Hu, A. Zhang, H. Chen, and M. Jin. 2009. Identification and characterization of novel immunogenic outer membrane proteins of Haemophilus parasuis serovar 5. Vaccine 27:5271-5277.","journal-title":"Vaccine"}],"container-title":["Clinical and Vaccine Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/CVI.00314-10","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/CVI.00314-10","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,2,26]],"date-time":"2025-02-26T12:34:59Z","timestamp":1740573299000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/CVI.00314-10"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,1]]},"references-count":45,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2011,1]]}},"alternative-id":["10.1128\/CVI.00314-10"],"URL":"https:\/\/doi.org\/10.1128\/cvi.00314-10","relation":{},"ISSN":["1556-6811","1556-679X"],"issn-type":[{"value":"1556-6811","type":"print"},{"value":"1556-679X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,1]]},"assertion":[{"value":"2010-07-21","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2010-09-21","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2011-01-05","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}