{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T17:46:22Z","timestamp":1793123182314,"version":"4.5.0"},"reference-count":19,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2010,12,1]],"date-time":"2010-12-01T00:00:00Z","timestamp":1291161600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Vaccine Immunol"],"published-print":{"date-parts":[[2010,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Twenty Hereford heifers approximately 9 months of age were vaccinated with saline (control) or 2 \u00d7 10<jats:sup>10<\/jats:sup>CFU of the<jats:italic>Brucella abortus<\/jats:italic>strain RB51 (RB51) vaccine. Immunologic responses after inoculation demonstrated significantly greater (<jats:italic>P<\/jats:italic>&lt; 0.05) antibody and proliferative responses to RB51 antigens in cattle vaccinated with RB51 than in the controls. Pregnant cattle received a conjunctival challenge at approximately 6 months of gestation with 10<jats:sup>7<\/jats:sup>CFU of<jats:italic>B. suis<\/jats:italic>bv. 1 strains isolated from naturally infected cattle. The fluorescence polarization assay and the buffered acid plate agglutination test had the highest sensitivities in detecting<jats:italic>B. suis<\/jats:italic>-infected cattle between 2 and 12 weeks after experimental infection. Serologic responses and lymphocyte proliferative responses to<jats:italic>B. suis<\/jats:italic>antigens did not differ between control and RB51 vaccinees after experimental infection. No abortions occurred in cattle in either treatment group after challenge, although there appeared to be an increased incidence of retained placenta after parturition in both the control and the RB51 vaccination treatment groups. Our data suggest that the mammary gland is a preferred site for<jats:italic>B. suis<\/jats:italic>localization in cattle. Vaccination with RB51 did not reduce<jats:italic>B. suis<\/jats:italic>infection rates in maternal or fetal tissues. In conclusion, although<jats:italic>B. suis<\/jats:italic>is unlikely to cause abortions and fetal losses in cattle, our data suggest that RB51 vaccination will not protect cattle against<jats:italic>B. suis<\/jats:italic>infection after exposure.<\/jats:p>","DOI":"10.1128\/cvi.00326-10","type":"journal-article","created":{"date-parts":[[2010,10,14]],"date-time":"2010-10-14T07:13:46Z","timestamp":1287040426000},"page":"1891-1895","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":21,"title":["Immune Responses and Protection against Experimental<i>Brucella suis<\/i>Biovar 1 Challenge in Nonvaccinated or<i>B. abortus<\/i>Strain RB51-Vaccinated Cattle"],"prefix":"10.1128","volume":"17","author":[{"given":"S. C.","family":"Olsen","sequence":"first","affiliation":[{"name":"Infectious Bacterial Diseases of Livestock Research Unit, National Animal Disease Center, Agricultural Research Service, United States Department of Agriculture, Ames, Iowa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S. G.","family":"Hennager","sequence":"additional","affiliation":[{"name":"National Veterinary Service Laboratory, Animal and Plant Health Inspection Service, United States Department of Agriculture, Ames, Iowa"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"17","year":"1988","unstructured":"Alton, G. G., L. M. Jones, R. D. Angus, and J. M. Verger. 1988. Techniques for the brucellosis laboratory, p. 17-136. 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