{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T15:55:44Z","timestamp":1761580544670,"version":"3.33.0"},"reference-count":24,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2007,12,1]],"date-time":"2007-12-01T00:00:00Z","timestamp":1196467200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Vaccine Immunol"],"published-print":{"date-parts":[[2007,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>The objectives of this study were to test the efficacy and safety of planned exposure to porcine reproductive and respiratory syndrome virus (PRRSV) in protecting nai\u0308ve and previously exposed pigs against PRRSV challenge and to gain information on the dose of PRRSV necessary to induce a protective immune response. Fifty 2-week-old pigs were randomly assigned to one of five groups: a group exposed to a low dose of autogenous PRRSV vaccine (the L-VAC group), a group exposed to a high dose of autogenous vaccine (the H-VAC group), a group exposed to a low dose of a heterologous PRRSV strain (strain SDSU73) prior to planned exposure (the SDSU73-L-VAC group), a group exposed to a high dose of a heterologous PRRSV strain (strain SDSU73) prior to planned exposure (the SDSU73-H-VAC group), and a control group. All groups were challenged with PRRSV VR2385 5 weeks after the planned exposure. Necropsy was done 2 weeks after the PRRSV challenge. The H-VAC, SDSU73-L-VAC, and SDSU73-H-VAC groups had significantly (<jats:italic>P<\/jats:italic>&lt; 0.05) less severe clinical disease (sneezing, respiratory scores, and weight gain), significantly (<jats:italic>P<\/jats:italic>&lt; 0.05) less severe macroscopic and microscopic lung lesions, and significantly (<jats:italic>P<\/jats:italic>&lt; 0.05) lower numbers of PRRSV genomic copy numbers in their sera compared to the results for the control group. Planned exposure to live PRRSV can be used as an inexpensive and effective way to decrease the severity of PRRSV-induced disease following subsequent challenge.<\/jats:p>","DOI":"10.1128\/cvi.00332-07","type":"journal-article","created":{"date-parts":[[2007,10,11]],"date-time":"2007-10-11T00:54:37Z","timestamp":1192064077000},"page":"1572-1577","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":15,"title":["Use of an Experimental Model To Test the Efficacy of Planned Exposure to Live Porcine Reproductive and Respiratory Syndrome Virus"],"prefix":"10.1128","volume":"14","author":[{"given":"Tanja","family":"Opriessnig","sequence":"first","affiliation":[{"name":"Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, Iowa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rodney B.","family":"Baker","sequence":"additional","affiliation":[{"name":"Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, Iowa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patrick G.","family":"Halbur","sequence":"additional","affiliation":[{"name":"Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, Iowa"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"147","volume":"10","year":"2001","unstructured":"Batista, L., C. Pijoan, and M. Torremorell. 2001. Experimental injection of gilts with porcine reproductive and respiratory syndrome virus (PRRSV) during acclimatization. J. Swine Health Prod.10:147-150.","journal-title":"J. Swine Health Prod."},{"key":"e_1_3_2_3_2","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1177\/104063879200400202","volume":"4","year":"1992","unstructured":"Benfield, D. A., E. Nelson, J. E. Collins, L. Harris, S. M. Goyal, D. Robison, W. T. Christianson, R. B. Morrison, D. Gorcyca, and D. Chladek. 1992. Characterization of swine infertility and respiratory syndrome (SIRS) virus (isolate ATCC VR-2332). J. Vet. Diagn. Investig.4:127-133.","journal-title":"J. Vet. Diagn. Investig."},{"key":"e_1_3_2_4_2","first-page":"629","volume":"142","year":"1997","unstructured":"Cavanagh, D. 1997. Nidovirales: a new order comprising Coronaviridae and Arteriviridae. Arch. Virol.142:629-633.","journal-title":"Arch. Virol."},{"key":"e_1_3_2_5_2","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1177\/104063879200400201","volume":"4","year":"1992","unstructured":"Collins, J. E., D. A. Benfield, W. T. Christianson, L. Harris, J. C. Hennings, D. P. Shaw, S. M. Goyal, S. McCullough, R. B. Morrison, H. S. Joo, D. Gorcyca, and D. Chladek. 1992. Isolation of swine infertility and respiratory syndrome virus (isolate ATCC VR-2332) in North America and experimental reproduction of the disease in gnotobiotic pigs. J. Vet. Diagn. Investig.4:117-126.","journal-title":"J. Vet. Diagn. Investig."},{"key":"e_1_3_2_6_2","first-page":"71","volume":"69","year":"2005","unstructured":"Fano, E., L. Olea, and C. Pijoan. 2005. Eradication of porcine reproductive and respiratory syndrome virus by serum inoculation of na\u00efve gilts. Can. J. Vet. Res.69:71-74.","journal-title":"Can. J. Vet. Res."},{"key":"e_1_3_2_7_2","first-page":"73","volume":"5","year":"1997","unstructured":"Halbur, P. G., and E. Bush. 1997. Update on abortion storms and sow mortality. J. Swine Health Prod.5:73.","journal-title":"J. Swine Health Prod."},{"key":"e_1_3_2_8_2","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1177\/030098589603300205","volume":"33","year":"1996","unstructured":"Halbur, P. G., P. S. Paul, M. L. Frey, J. Landgraf, K. Eernisse, X. J. Meng, J. J. Andrews, M. A. Lum, and J. A. Rathje. 1996. Comparison of the antigen distribution of two US porcine reproductive and respiratory syndrome virus isolates with that of the Lelystad virus. Vet. Pathol.33:159-170.","journal-title":"Vet. Pathol."},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1177\/030098589503200606"},{"key":"e_1_3_2_10_2","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1177\/104063879600800103","volume":"8","year":"1996","unstructured":"Halbur, P. G., P. S. Paul, X. J. Meng, M. A. Lum, J. J. Andrews, and J. A. Rathje. 1996. Comparative pathogenicity of nine US porcine reproductive and respiratory syndrome virus (PRRSV) isolates in a five-week-old cesarean-derived, colostrum-deprived pig model. J. Vet. Diagn. Investig.8:11-20.","journal-title":"J. Vet. Diagn. Investig."},{"key":"e_1_3_2_11_2","first-page":"369","year":"2004","unstructured":"Hill, H. T., J. A. Mulford, J. J. Kaisand, A. Holtcamp, C. Reyes. 2004. PRRS control: to hell and back. Proceedings of the American Association of Swine Veterinarians, 6 to 9 March 2004, p. 369-375. American Association of Swine Veterinarians, Perry, IA.","journal-title":"Proceedings of the American Association of Swine Veterinarians, 6 to 9 March 2004"},{"key":"e_1_3_2_12_2","doi-asserted-by":"crossref","first-page":"103","DOI":"10.54846\/jshap\/280","volume":"9","year":"2001","unstructured":"Hurd, H. S., E. J. Bush, W. Losinger, B. Corso, J. J. Zimmerman, R. Wills, S. Swenson, D. Pyburn, P. Yeske, and T. Burkgren. 2001. Outbreaks of porcine reproductive failure: report on a collaborative field investigation. J. Swine Health Prod.9:103-108.","journal-title":"J. Swine Health Prod."},{"key":"e_1_3_2_13_2","first-page":"1","volume":"1","year":"1989","unstructured":"Keffaber, K. 1989. Reproductive failure of unknown aetiology. Am. Assoc. Swine Pract. Newsl.1:1-10.","journal-title":"Am. Assoc. Swine Pract. Newsl."},{"key":"e_1_3_2_14_2","doi-asserted-by":"crossref","first-page":"1022","DOI":"10.2460\/ajvr.1999.60.08.1022","volume":"60","year":"1999","unstructured":"Lager, K. M., W. L. Mengeling, and S. L. Brockmeier. 1999. Evaluation of protective immunity in gilts inoculated with the NADC-8 isolate of porcine reproductive and respiratory syndrome virus (PRRSV) and challenge-exposed with an antigenically distinct PRRSV isolate. Am. J. Vet. Res.60:1022-1027.","journal-title":"Am. J. Vet. Res."},{"key":"e_1_3_2_15_2","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1016\/S0378-1135(00)00196-6","volume":"74","year":"2000","unstructured":"Meng, X. J. 2000. Heterogeneity of porcine reproductive and respiratory syndrome virus: implications for current vaccine efficacy and future vaccine development. Vet. Microbiol.74:309-329.","journal-title":"Vet. Microbiol."},{"key":"e_1_3_2_16_2","doi-asserted-by":"crossref","first-page":"1540","DOI":"10.2460\/ajvr.1998.59.12.1540","volume":"59","year":"1998","unstructured":"Mengeling, W. L., K. M. Lager, and A. C. Vorwald. 1998. Clinical consequences of exposing pregnant gilts to strains of porcine reproductive and respiratory syndrome (PRRS) virus isolated from field cases of \u201catypical\u201d PRRS. Am. J. Vet. Res.59:1540-1544.","journal-title":"Am. J. Vet. Res."},{"key":"e_1_3_2_17_2","first-page":"33","volume":"9","year":"2002","unstructured":"Nawagitgul, P., P. A. Harms, I. Morozov, B. J. Thacker, S. D. Sorden, C. Lekcharoensuk, and P. S. Paul. 2002. Modified indirect porcine circovirus (PCV) type 2-based and recombinant capsid protein (ORF2)-based enzyme-linked immunosorbent assays for detection of antibodies to PCV. Clin. Diagn. Lab. Immunol.9:33-40.","journal-title":"Clin. Diagn. Lab. Immunol."},{"key":"e_1_3_2_18_2","doi-asserted-by":"crossref","first-page":"385","DOI":"10.2460\/javma.2005.227.385","volume":"227","year":"2005","unstructured":"Neumann, E. J., J. B. Kliebenstein, C. D. Johnson, J. W. Mabry, E. J. Bush, A. H. Seitzinger, A. L. Green, and J. J. Zimmerman. 2005. Assessment of the economic impact of porcine reproductive and respiratory syndrome on swine production in the United States. J. Am. Vet. Med. Assoc.227:385-392.","journal-title":"J. Am. Vet. Med. Assoc."},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.76.23.11837-11844.2002"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00074-06"},{"key":"e_1_3_2_21_2","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1006\/viro.2002.1612","volume":"302","year":"2002","unstructured":"Osorio, F. A., J. A. Galeota, E. Nelson, B. Brodersen, A. Doster, R. Wills, F. Zuckermann, and W. W. Laegreid. 2002. Passive transfer of virus-specific antibodies confers protection against reproductive failure induced by a virulent strain of porcine reproductive and respiratory syndrome virus and establishes sterilizing immunity. Virology302:9-20.","journal-title":"Virology"},{"key":"e_1_3_2_22_2","first-page":"33","volume":"36","year":"2005","unstructured":"Pugh, M. L., R. Main, N. DeBuse, and L. Karriker. 2005. Development of a quality-controlled protocol and resulting commercial sow farm production for on-farm live PRRSV virus inoculation. Proc. Am. Assoc. Swine Pract.36:33-36.","journal-title":"Proc. Am. Assoc. Swine Pract."},{"key":"e_1_3_2_23_2","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1354\/vp.37-2-143","volume":"37","year":"2000","unstructured":"Thanawongnuwech, R., G. B. Brown, P. G. Halbur, J. A. Roth, R. L. Royer, and B. J. Thacker. 2000. Pathogenesis of porcine reproductive and respiratory syndrome virus-induced increase in susceptibility to Streptococcus suis infection. Vet. Pathol.37:143-152.","journal-title":"Vet. Pathol."},{"key":"e_1_3_2_24_2","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1080\/01652176.1991.9694296","volume":"13","year":"1991","unstructured":"Wensvoort, G., C. Terpstra, J. M. Pol, E. A. ter Laak, M. Bloemraad, E. P. de Kluyver, C. Kragten, L. van Buiten, A. den Besten, and F. Wagenaar. 1991. Mystery swine disease in The Netherlands: the isolation of Lelystad virus. Vet. Q.13:121-130.","journal-title":"Vet. Q."},{"key":"e_1_3_2_25_2","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1177\/104063879500700302","volume":"7","year":"1995","unstructured":"Yoon, K. J., J. J. Zimmerman, S. L. Swenson, M. J. McGinley, K. A. Eernisse, A. Brevik, L. L. Rhinehart, M. L. Frey, H. T. Hill, and K. B. Platt. 1995. Characterization of the humoral immune response to porcine reproductive and respiratory syndrome (PRRS) virus infection. J. Vet. Diagn. Investig.7:305-312.","journal-title":"J. Vet. Diagn. Investig."}],"container-title":["Clinical and Vaccine Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/CVI.00332-07","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/CVI.00332-07","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,1,21]],"date-time":"2025-01-21T15:30:28Z","timestamp":1737473428000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/CVI.00332-07"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,12]]},"references-count":24,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2007,12]]}},"alternative-id":["10.1128\/CVI.00332-07"],"URL":"https:\/\/doi.org\/10.1128\/cvi.00332-07","relation":{},"ISSN":["1556-6811","1556-679X"],"issn-type":[{"type":"print","value":"1556-6811"},{"type":"electronic","value":"1556-679X"}],"subject":[],"published":{"date-parts":[[2007,12]]},"assertion":[{"value":"2007-08-10","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2007-10-01","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-12-18","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}