{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,7]],"date-time":"2026-03-07T23:16:23Z","timestamp":1772925383882,"version":"3.50.1"},"reference-count":34,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2010,1,1]],"date-time":"2010-01-01T00:00:00Z","timestamp":1262304000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Vaccine Immunol"],"published-print":{"date-parts":[[2010,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The conventional means of diagnosis of histoplasmosis presents difficulties because of the delay to the time that the diagnosis is made, indicating the need for the implementation of molecular assays. We evaluated 146 clinical samples from 135 patients suspected of having histoplasmosis using a previously reported nested PCR assay for the\n            <jats:italic>H<\/jats:italic>\n            <jats:italic>istoplasma<\/jats:italic>\n            <jats:italic>capsulatum<\/jats:italic>\n            -specific 100-kDa protein (the Hc100 PCR). In order to determine the specificity of this molecular test, we also used samples from healthy individuals (\n            <jats:italic>n<\/jats:italic>\n            = 20), patients suspected of having respiratory disease with negative fungal cultures (\n            <jats:italic>n<\/jats:italic>\n            = 29), and patients with other proven infections (\n            <jats:italic>n<\/jats:italic>\n            = 60). Additionally, a sizable collection of DNA from cultures of\n            <jats:italic>H<\/jats:italic>\n            .\n            <jats:italic>capsulatum<\/jats:italic>\n            and other medically relevant pathogens was studied. A panfungal PCR assay that amplified the internal transcribed spacer 2 region was also used to identify all fungal DNAs. All PCR-amplified products were sequenced. Of the 146 clinical samples, 67 (45.9%) were positive by culture and PCR, while 9 samples negative by culture were positive by PCR. All the sequences corresponding to the 76 amplified products presented \u226598% identity with\n            <jats:italic>H.<\/jats:italic>\n            <jats:italic>capsulatum<\/jats:italic>\n            . The Hc100 PCR exhibited a sensitivity of 100% and specificities of 92.4% and 95.2% when the results were compared to those for the negative controls and samples from other proven clinical entities, respectively; the positive predictive value was 83% and the negative predictive value was 100%; the positive and negative likelihood rates were 25 and 0, respectively. These results suggest that the Hc100 nested PCR assay for the detection of\n            <jats:italic>H.<\/jats:italic>\n            <jats:italic>capsulatum<\/jats:italic>\n            DNA is a useful test in areas where mycosis caused by this organism is endemic.\n          <\/jats:p>","DOI":"10.1128\/cvi.00332-09","type":"journal-article","created":{"date-parts":[[2009,11,26]],"date-time":"2009-11-26T04:10:17Z","timestamp":1259208617000},"page":"62-67","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":47,"title":["Validation and Clinical Application of a Molecular Method for Identification of\n            <i>Histoplasma capsulatum<\/i>\n            in Human Specimens in Colombia, South America"],"prefix":"10.1128","volume":"17","author":[{"given":"Cesar","family":"Mun\u0303oz","sequence":"first","affiliation":[{"name":"Medical and Experimental Mycology Unit, Corporacio\u0301n para Investigaciones Biolo\u0301gicas (CIB), Medelli\u0301n, Colombia, and Escuela de Microbiologi\u0301a, Universidad de Antioquia, Medelli\u0301n, Colombia"},{"name":"Mycotic Diseases Branch, Centers for Disease Control and Prevention, Atlanta, Georgia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Beatriz L.","family":"Go\u0301mez","sequence":"additional","affiliation":[{"name":"Mycotic Diseases Branch, Centers for Disease Control and Prevention, Atlanta, Georgia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Angela","family":"Tobo\u0301n","sequence":"additional","affiliation":[{"name":"Medical and Experimental Mycology Unit, Corporacio\u0301n para Investigaciones Biolo\u0301gicas (CIB), Medelli\u0301n, Colombia, and Escuela de Microbiologi\u0301a, Universidad de Antioquia, Medelli\u0301n, Colombia"},{"name":"Hospital La Mari\u0301a, Medelli\u0301n, Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karen","family":"Arango","sequence":"additional","affiliation":[{"name":"Medical and Experimental Mycology Unit, Corporacio\u0301n para Investigaciones Biolo\u0301gicas (CIB), Medelli\u0301n, Colombia, and Escuela de Microbiologi\u0301a, Universidad de Antioquia, Medelli\u0301n, Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Angela","family":"Restrepo","sequence":"additional","affiliation":[{"name":"Medical and Experimental Mycology Unit, Corporacio\u0301n para Investigaciones Biolo\u0301gicas (CIB), Medelli\u0301n, Colombia, and Escuela de Microbiologi\u0301a, Universidad de Antioquia, Medelli\u0301n, Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Margarita M.","family":"Correa","sequence":"additional","affiliation":[{"name":"Molecular Microbiology Group, Escuela de Microbiologi\u0301a, Universidad de Antioquia, Medelli\u0301n, Colombia"},{"name":"Escuela de Microbiologi\u0301a, Universidad de Antioquia, Medelli\u0301n, Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carlos","family":"Muskus","sequence":"additional","affiliation":[{"name":"Programa de Estudio y Control de Enfermedades Tropicales (PECET), Universidad de Antioquia, Medelli\u0301n, Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luz Elena","family":"Cano","sequence":"additional","affiliation":[{"name":"Medical and Experimental Mycology Unit, Corporacio\u0301n para Investigaciones Biolo\u0301gicas (CIB), Medelli\u0301n, Colombia, and Escuela de Microbiologi\u0301a, Universidad de Antioquia, Medelli\u0301n, Colombia"},{"name":"Escuela de Microbiologi\u0301a, Universidad de Antioquia, Medelli\u0301n, Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Angel","family":"Gonza\u0301lez","sequence":"additional","affiliation":[{"name":"Medical and Experimental Mycology Unit, Corporacio\u0301n para Investigaciones Biolo\u0301gicas (CIB), Medelli\u0301n, Colombia, and Escuela de Microbiologi\u0301a, Universidad de Antioquia, Medelli\u0301n, Colombia"},{"name":"Escuela de Microbiologi\u0301a, Universidad de Antioquia, Medelli\u0301n, Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1007\/BF00439115","volume":"133","year":"1996","unstructured":"Benard, G., R. C. Gryschek, A. J. Duarte, and M. A. Shikanai-Yasuda. 1996. Cryptococcosis as an opportunistic infection in immunodeficiency secondary to paracoccidioidomycosis. Mycopathologia133:65-69.","journal-title":"Mycopathologia"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.4.1506-1509.2001"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.5.1644-1647.2002"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.4.1753-1755.2003"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.3.1456-1458.2005"},{"key":"e_1_3_2_7_2","doi-asserted-by":"crossref","first-page":"665","DOI":"10.1007\/s10096-006-0207-y","volume":"25","year":"2006","unstructured":"Buitrago, M. J., J. Berenguer, E. Mellado, J. L. Rodr\u00edguez-Tudela, and M. Cuenca-Estrella. 2006. Detection of imported histoplasmosis in serum of HIV-infected patients using a real-time PCR-based assay. Eur. J. Clin. Microbiol. Infect. Dis.25:665-668.","journal-title":"Eur. J. Clin. Microbiol. Infect. Dis."},{"key":"e_1_3_2_8_2","first-page":"109","volume":"21","year":"1971","unstructured":"Carmona, J. 1971. \u00c1nalisis estad\u00edstico y ecoepidemiol\u00f3gico de la sensibilidad a la histoplasmina en Colombia, 1950-1968. Antioquia Med.21:109-154.","journal-title":"Antioquia Med."},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00071-07"},{"key":"e_1_3_2_10_2","doi-asserted-by":"crossref","first-page":"443","DOI":"10.1097\/01.qco.0000244049.15888.b9","volume":"19","year":"2006","unstructured":"Couppie, P., C. Azna, B. Carme, and M. Nacher. 2006. American histoplasmosis in developing countries with a special focus on patients with HIV: diagnosis, treatment, and prognosis. Curr. Opin. Infect. Dis.19:443-449.","journal-title":"Curr. Opin. Infect. Dis."},{"key":"e_1_3_2_11_2","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1007\/BF01717448","volume":"10","year":"1994","unstructured":"Dujardin, B., J. Van der Ende, A. Van Gompel, J. P. Unger, and P. Van der Stuyft. 1994. Likelihood ratios: a real improvement for clinical decision making? Eur. J. Epidemiol.10:29-36.","journal-title":"Eur. J. Epidemiol."},{"key":"e_1_3_2_12_2","first-page":"115","year":"1975","unstructured":"Galen, R. S., and S. R. Gambino. 1975. Beyond normality. The predictive value and efficiency of medical diagnosis, p. 115-116. John Wiley & Sons, Inc., New York, NY.","journal-title":"Beyond normality."},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.37.3.675-680.1999"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.35.10.2618-2622.1997"},{"key":"e_1_3_2_15_2","first-page":"929","volume":"117","year":"1978","unstructured":"Goodwin, R. A., Jr., and R. M. Des Prez. 1978. State of the art: histoplasmosis. Am. Rev. Respir. Dis.117:929-956.","journal-title":"Am. Rev. Respir. Dis."},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.2.535-539.2003"},{"key":"e_1_3_2_17_2","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1590\/S1517-83822006000100001","volume":"37","year":"2006","unstructured":"Guimaraes, A. J., J. D. Nosanchuk, and R. M. Zancope-Oliveira. 2006. Diagnosis of histoplasmosis. Braz. J. Microbiol.37:1-13.","journal-title":"Braz. J. Microbiol."},{"key":"e_1_3_2_18_2","doi-asserted-by":"crossref","first-page":"1047","DOI":"10.1097\/QAD.0b013e3282ffde67","volume":"22","year":"2008","unstructured":"Huber, F., M. Nacher, C. Aznar, M. Pierre-Demar, M. El Guedj, T. Vaz, V. Vantilcke, A. Mahamat, C. Magnien, E. Chauvet, B. Carme, and P. Couppi\u00e9. 2008. AIDS-related Histoplasma capsulatum var. capsulatum infection: 25 years experience of French Guiana. AIDS22:1047-1053.","journal-title":"AIDS"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00027-06"},{"key":"e_1_3_2_20_2","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1016\/j.ccm.2009.02.002","volume":"30","year":"2009","unstructured":"Kauffman, C. A. 2009. Histoplasmosis. Clin. Chest Med.30:217-225.","journal-title":"Clin. Chest Med."},{"key":"e_1_3_2_21_2","doi-asserted-by":"crossref","first-page":"286","DOI":"10.1016\/S0399-077X(04)00146-5","volume":"34","year":"2004","unstructured":"Lewden, C., M. Sobesky, A. Cabie, P. Couppie, F. Boulard, F. Bissuel, T. May, P. Morlat, G. Chene, I. Lamaury, and D. Salmon. 2004. Causes of death among HIV infected adults in French Guyana and the French West Indies in the era of highly active antiretroviral therapy (HAART). Med. Mal. Infect.34:286-292.","journal-title":"Med. Mal. Infect."},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.3.1295-1298.2003"},{"key":"e_1_3_2_23_2","doi-asserted-by":"crossref","first-page":"441","DOI":"10.1016\/j.diagmicrobio.2007.03.008","volume":"58","year":"2007","unstructured":"Maubon, D., S. Simon, and C. Aznar. 2007. Histoplasmosis diagnosis using a polymerase chain reaction method. Application on human samples in French Guiana, South America. Diagn. Microbiol. Infect. Dis.58:441-444.","journal-title":"Diagn. Microbiol. Infect. Dis."},{"key":"e_1_3_2_24_2","first-page":"93","volume":"64","year":"2009","unstructured":"Pellaton, C., M. Cavassini, K. Jaton-Ogay, P. N. Carron, S. Christen-Zaech, T. Calandra, J. Bille, and P. M. Hauser. 2009. Histoplasma capsulatum var. duboisii infection in a patient with AIDS: rapid diagnosis using polymerase chain reaction-sequencing. Diagn. Microbiol. Infect. Dis.64:93-97.","journal-title":"Diagn. Microbiol. Infect. Dis."},{"key":"e_1_3_2_25_2","first-page":"27","volume":"17","year":"1968","unstructured":"Restrepo, A., M. Robledo, S. Ospina, M. Restrepo, and A. Correa. 1968. Distribution of paracoccidioidin and histoplasmin sensitivity in Colombia. Am. J. Trop. Med. Hyg.17:27-37.","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"e_1_3_2_26_2","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1016\/S0891-5520(20)30577-8","volume":"8","year":"1994","unstructured":"Rios-Fabra, A., A. Restrepo, and A. Isturiz. 1994. Fungal infection in Latin American countries. Infect. Dis. Clin. North Am.8:129-154.","journal-title":"Infect. Dis. Clin. North Am."},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00066-09"},{"key":"e_1_3_2_28_2","doi-asserted-by":"crossref","first-page":"576","DOI":"10.4269\/ajtmh.2005.73.576","volume":"73","year":"2005","unstructured":"Tob\u00f3n, A. M., C. A. Agudelo, D. S. Rosero, J. E. Ochoa, C. Bedout, A. Zuluaga, M. Arango, L. E. Cano, J. Sampedro, and A. Restrepo. 2005. Disseminated histoplasmosis: a comparative study between patients with acquired immunodeficiency syndrome and non-human immunodeficiency virus-infected individuals. Am. J. Trop. Med. Hyg.73:576-582.","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"e_1_3_2_29_2","doi-asserted-by":"crossref","first-page":"488","DOI":"10.1016\/j.tim.2003.08.007","volume":"11","year":"2003","unstructured":"Wheat, L. J. 2003. Current diagnosis of histoplasmosis. Trends Microbiol.11:488-494.","journal-title":"Trends Microbiol."},{"key":"e_1_3_2_30_2","doi-asserted-by":"crossref","first-page":"274","DOI":"10.1111\/j.1439-0507.2006.01253.x","volume":"49","year":"2006","unstructured":"Wheat, L. J. 2006. Histoplasmosis: a review for clinicians from non-endemic areas. Mycoses49:274-282.","journal-title":"Mycoses"},{"key":"e_1_3_2_31_2","doi-asserted-by":"crossref","first-page":"1207","DOI":"10.1517\/14712598.6.11.1207","volume":"6","year":"2006","unstructured":"Wheat, L. J. 2006. Improvements in diagnosis of histoplasmosis. Expert Opin. Biol. Ther.6:1207-1221.","journal-title":"Expert Opin. Biol. Ther."},{"key":"e_1_3_2_32_2","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1111\/j.1399-3062.2006.00165.x","volume":"8","year":"2006","unstructured":"Wheat, L. J. 2006. Antigen detection, serology, and molecular diagnosis of invasive mycoses in the immunocompromised host. Transpl. Infect. Dis.8:128-139.","journal-title":"Transpl. Infect. Dis."},{"key":"e_1_3_2_33_2","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0891-5520(02)00039-9","volume":"17","year":"2003","unstructured":"Wheat, L. J., and C. A. Kauffman. 2003. Histoplasmosis. Infect. Dis. Clin. North Am.17:1-19.","journal-title":"Infect. Dis. Clin. North Am."},{"key":"e_1_3_2_34_2","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1097\/00005792-198407000-00002","volume":"63","year":"1984","unstructured":"Wheat, L. J., J. Wass, J. Norton, R. B. Kohler, and M. L. French. 1984. Cavitary histoplasmosis occurring during two large urban outbreaks. Analysis of clinical, epidemiologic, roentgenographic, and laboratory features. Medicine63:201-209.","journal-title":"Medicine"},{"key":"e_1_3_2_35_2","first-page":"315","year":"1990","unstructured":"White, T. J., T. Bruns, S. Lee, and J. Taylor. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics, p. 315-322. In M. A. Innis, D. H. Gelfand, J. J. Sninsky, and T. J. White (ed.), PCR protocols: a guide to methods and applications. Academic Press Inc., San Diego, CA.","journal-title":"PCR protocols: a guide to methods and applications"}],"container-title":["Clinical and Vaccine Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/CVI.00332-09","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/CVI.00332-09","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,7,29]],"date-time":"2021-07-29T20:42:38Z","timestamp":1627591358000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/CVI.00332-09"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,1]]},"references-count":34,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2010,1]]}},"alternative-id":["10.1128\/CVI.00332-09"],"URL":"https:\/\/doi.org\/10.1128\/cvi.00332-09","relation":{},"ISSN":["1556-6811","1556-679X"],"issn-type":[{"value":"1556-6811","type":"print"},{"value":"1556-679X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,1]]},"assertion":[{"value":"2009-08-16","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2009-11-16","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-12-18","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}