{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T11:04:09Z","timestamp":1782817449420,"version":"3.54.5"},"reference-count":23,"publisher":"American Society for Microbiology","issue":"11","license":[{"start":{"date-parts":[[2010,11,1]],"date-time":"2010-11-01T00:00:00Z","timestamp":1288569600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Vaccine Immunol"],"published-print":{"date-parts":[[2010,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>The goal of this study was to evaluate the BioPlex 2200<jats:italic>Toxoplasma<\/jats:italic>, rubella, and cytomegalovirus (CMV) (ToRC) IgG and IgM multiplex immunoassays (Bio-Rad Laboratories, Hercules, CA) and compare the results to those of conventional testing by enzyme immunoassay (EIA) and enzyme-linked fluorescent assay (ELFA). Serum specimens (<jats:italic>n<\/jats:italic>= 600) submitted for routine ToRC IgG and IgM testing by EIA (SeraQuest, Doral, FL; Diamedix, Miami, FL) or ELFA (Vidas; bioMe\u0301rieux, Durham, NC) were also tested by the BioPlex ToRC multiplex immunoassays. Samples showing discordant results were retested by both methods, with further discrepancies being arbitrated by a third assay. Following repeat testing, the BioPlex<jats:italic>Toxoplasma<\/jats:italic>, rubella, and CMV IgG assays demonstrated agreements of 98.7 (592\/600 specimens), 93.3 (560\/600 specimens), and 98.3% (590\/600 specimens), respectively, while the ToRC IgM assays yielded agreements of 91.2 (547\/600 specimens), 87.3 (524\/600 specimens), and 95.2% (571\/600 specimens), respectively. The BioPlex ToRC IgG assays provided results comparable to EIA\/ELFA results, with kappa coefficients showing near-perfect agreement for the<jats:italic>Toxoplasma<\/jats:italic>(\u03ba = 0.94) and CMV (\u03ba = 0.97) IgG assays and substantial agreement for the rubella IgG assay (\u03ba = 0.66). The BioPlex ToRC IgM assays showed lower specificity with only slight agreement for<jats:italic>Toxoplasma<\/jats:italic>IgM (\u03ba = 0.07), poor agreement for rubella IgM (\u03ba = \u22120.03), and moderate agreement for CMV IgM (\u03ba = 0.55). Both the BioPlex IgG and IgM assays reduced turnaround time (1.7 h versus 5.5 h by EIA\/ELFA for 100 specimens) and eliminated the necessity to manually pipette or aliquot specimens prior to testing.<\/jats:p>","DOI":"10.1128\/cvi.00332-10","type":"journal-article","created":{"date-parts":[[2010,9,23]],"date-time":"2010-09-23T04:39:46Z","timestamp":1285216786000},"page":"1734-1738","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":45,"title":["Multiplex Detection of IgM and IgG Class Antibodies to<i>Toxoplasma gondii<\/i>, Rubella Virus, and Cytomegalovirus Using a Novel Multiplex Flow Immunoassay"],"prefix":"10.1128","volume":"17","author":[{"given":"M. J.","family":"Binnicker","sequence":"first","affiliation":[{"name":"Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic and Mayo Clinic College of Medicine, Rochester, Minnesota 55905"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D. J.","family":"Jespersen","sequence":"additional","affiliation":[{"name":"Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic and Mayo Clinic College of Medicine, Rochester, Minnesota 55905"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. A.","family":"Harring","sequence":"additional","affiliation":[{"name":"Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic and Mayo Clinic College of Medicine, Rochester, Minnesota 55905"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"1028","DOI":"10.1002\/pd.1242","volume":"25","year":"2005","unstructured":"Abdel-Fattah, S. A., A. Bhat, S. Illanes, J. L. Bartha, and D. Carrington. 2005. TORCH test for fetal medicine indications: only CMV is necessary in the United Kingdom. Prenat. Diagn.25:1028-1031.","journal-title":"Prenat. Diagn."},{"key":"e_1_3_2_3_2","doi-asserted-by":"crossref","first-page":"S151","DOI":"10.1086\/505948","volume":"43","year":"2006","unstructured":"Averhoff, F., J. Zucker, C. Vellozzi, S. Redd, C. Woodfill, S. Waterman, J. Baggs, M. Weinberg, A. Rodriquez-Lainz, V. Carrion, C. Goto, and S. E. Reef. 2006. Adequacy of surveillance to detect endemic rubella transmission in the United States. Clin. Infect. Dis.43(Suppl. 3):S151-S157.","journal-title":"Clin. Infect. Dis."},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00372-09"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00325-09"},{"key":"e_1_3_2_6_2","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/0732-8893(94)90101-5","volume":"20","year":"1994","unstructured":"Doern, G. V., L. Robbie, and L. Marrama. 1994. Comparison of two enzyme immunoassays and two latex agglutination assays for detection of cytomegalovirus antibody. Diagn. Microbiol. Infect. Dis.20:109-112.","journal-title":"Diagn. Microbiol. Infect. Dis."},{"key":"e_1_3_2_7_2","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1016\/S1085-5629(05)80016-1","volume":"14","year":"1995","unstructured":"Epps, R. E., M. R. Pittelkow, and W. P. Su. 1995. TORCH syndrome. Semin. Dermatol.14:179-186.","journal-title":"Semin. Dermatol."},{"key":"e_1_3_2_8_2","doi-asserted-by":"crossref","first-page":"222","DOI":"10.4103\/0255-0857.53204","volume":"27","year":"2009","unstructured":"Gandhoke, I., R. Aggarwal, S. A. Hussain, S. T. Pasha, P. Sethi, S. Thakur, S. Lal, and S. Khare. 2009. Congenital CMV infection; diagnosis in symptomatic infants. Indian. J. Med. Microbiol.27:222-225.","journal-title":"Indian. J. Med. Microbiol."},{"key":"e_1_3_2_9_2","first-page":"346","volume":"159","year":"1993","unstructured":"Garland, S. M., and G. L. Gilbert. 1993. Investigation of congenital infection\u2014the TORCH screen is not a legitimate test. Paediatric Infectious Diseases Group of the Australasian Society for Infectious Diseases. Med. J. Aust.159:346-348.","journal-title":"Paediatric Infectious Diseases Group of the Australasian Society for Infectious Diseases. Med. J. Aust."},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00086-10"},{"key":"e_1_3_2_11_2","doi-asserted-by":"crossref","first-page":"360","DOI":"10.1002\/jmv.21077","volume":"80","year":"2008","unstructured":"Hardelid, P., D. Williams, C. Dezateux, W. D. Cubitt, C. S. Peckham, P. A. Tookey, and M. Cortina-Borja. 2008. Agreement of rubella IgG antibody measured in serum and dried blood spots using two commercial enzyme-linked immunosorbent assays. J. Med. Virol.80:360-364.","journal-title":"J. Med. Virol."},{"key":"e_1_3_2_12_2","first-page":"284","volume":"30","year":"1999","unstructured":"Kaur, R., N. Gupta, D. Nair, M. Kakkar, and M. D. Mathur. 1999. Screening for TORCH infections in pregnant women: a report from Delhi. Southeast Asian J. Trop. Med. Public Health30:284-286.","journal-title":"Southeast Asian J. Trop. Med. Public Health"},{"key":"e_1_3_2_13_2","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1055\/s-2000-9288","volume":"17","year":"2000","unstructured":"Khan, N. A., and S. N. Kazzi. 2000. Yield and costs of screening growth-retarded infants for torch infections. Am. J. Perinatol.17:131-135.","journal-title":"Am. J. Perinatol."},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.11.4996-5000.2004"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02172-08"},{"key":"e_1_3_2_16_2","doi-asserted-by":"crossref","first-page":"159","DOI":"10.2307\/2529310","volume":"33","year":"1977","unstructured":"Landis, J. R., and G. G. Koch. 1977. The measurement of observer agreement for categorical data. Biometrics33:159-174.","journal-title":"Biometrics"},{"key":"e_1_3_2_17_2","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1542\/peds.72.1.41","volume":"72","year":"1983","unstructured":"Leland, D., M. L. French, M. B. Kleiman, and R. L. Schreiner. 1983. The use of TORCH titers. Pediatrics72:41-43.","journal-title":"Pediatrics"},{"key":"e_1_3_2_18_2","first-page":"2954","year":"2009","unstructured":"McAuley, J., K. Boyer, J. Remington, and R. McLeod. 2009. Toxoplasmosis, p. 2954. In R. Feigin, J. Cherry, G. Demmler-Harrison, and S. Kaplan (ed.), Textbook of pediatric infectious diseases, 6th ed. Saunders, Philadelphia, PA.","journal-title":"Textbook of pediatric infectious diseases"},{"key":"e_1_3_2_19_2","doi-asserted-by":"crossref","first-page":"554","DOI":"10.1086\/590149","volume":"47","year":"2008","unstructured":"Montoya, J. G., and J. S. Remington. 2008. Management of Toxoplasma gondii infection during pregnancy. Clin. Infect. Dis.47:554-566.","journal-title":"Clin. Infect. Dis."},{"key":"e_1_3_2_20_2","doi-asserted-by":"crossref","first-page":"781","DOI":"10.1093\/clinids\/20.4.781","volume":"20","year":"1995","unstructured":"Montoya, J. G., and J. S. Remington. 1995. Studies on the serodiagnosis of toxoplasmic lymphadenitis. Clin. Infect. Dis.20:781-789.","journal-title":"Clin. Infect. Dis."},{"key":"e_1_3_2_21_2","doi-asserted-by":"crossref","first-page":"252","DOI":"10.1016\/j.clim.2003.11.017","volume":"110","year":"2004","unstructured":"Morgan, E., R. Varro, H. Sepulveda, J. A. Ember, J. Apgar, J. Wilson, L. Lowe, R. Chen, L. Shivraj, A. Agadir, R. Campos, D. Ernst, and A. Gaur. 2004. Cytometric bead array: a multiplexed assay platform with applications in various areas of biology. Clin. Immunol.110:252-266.","journal-title":"Clin. Immunol."},{"key":"e_1_3_2_22_2","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1097\/00003081-199903000-00012","volume":"42","year":"1999","unstructured":"Newton, E. R. 1999. Diagnosis of perinatal TORCH infections. Clin. Obstet. Gynecol.42:59-70; quiz, 174-175.","journal-title":"Clin. Obstet. Gynecol."},{"key":"e_1_3_2_23_2","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1016\/j.diagmicrobio.2005.04.005","volume":"53","year":"2005","unstructured":"Roux-Buisson, N., H. Fricker-Hidalgo, A. Foussadier, D. Rolland, A. S. Suchel-Jambon, M. P. Brenier-Pinchart, and H. Pelloux. 2005. Comparative analysis of the VIDAS Toxo IgG IV assay in the detection of antibodies to Toxoplasma gondii. Diagn. Microbiol. Infect. Dis.53:79-81.","journal-title":"Diagn. Microbiol. Infect. Dis."},{"key":"e_1_3_2_24_2","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1007\/978-1-59745-323-3_9","volume":"378","year":"2007","unstructured":"Varro, R., R. Chen, H. Sepulveda, and J. Apgar. 2007. Bead-based multianalyte flow immunoassays: the cytometric bead array system. Methods Mol. Biol.378:125-152.","journal-title":"Methods Mol. Biol."}],"container-title":["Clinical and Vaccine Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/CVI.00332-10","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/CVI.00332-10","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,6,3]],"date-time":"2023-06-03T12:49:38Z","timestamp":1685796578000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/CVI.00332-10"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,11]]},"references-count":23,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2010,11]]}},"alternative-id":["10.1128\/CVI.00332-10"],"URL":"https:\/\/doi.org\/10.1128\/cvi.00332-10","relation":{},"ISSN":["1556-6811","1556-679X"],"issn-type":[{"value":"1556-6811","type":"print"},{"value":"1556-679X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,11]]},"assertion":[{"value":"2010-08-16","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2010-09-16","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-12-18","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}