{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T13:15:47Z","timestamp":1791033347009,"version":"4.1.0"},"reference-count":64,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2008,1,1]],"date-time":"2008-01-01T00:00:00Z","timestamp":1199145600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Vaccine Immunol"],"published-print":{"date-parts":[[2008,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The liver fluke\n                    <jats:italic>Fasciola hepatica<\/jats:italic>\n                    parasitizes humans and ruminant livestock worldwide, and it is now being considered a reemerging zoonotic disease, especially in areas in which it is endemic, such as South America. This study investigates the immune response to excretory and secretory products produced by\n                    <jats:italic>F. hepatica<\/jats:italic>\n                    in a group of patients from the Peruvian Altiplano, where the disease is highly endemic. Using a proteomic approach and immunoblotting techniques, we have identified the enzymes leucine aminopeptidase (LAP) and phosphoenolpyruvate carboxykinase as immunodominant antigens recognized by sera from fasciolosis patients. An indirect enzyme-linked immunosorbent assay using recombinant LAP as the antigen was developed to check sera from individuals of this region. Our results demonstrate that LAP produces a specific and strong reaction, suggesting its potential use in the serologic diagnosis of\n                    <jats:italic>F. hepatica<\/jats:italic>\n                    infections in humans.\n                  <\/jats:p>","DOI":"10.1128\/cvi.00338-07","type":"journal-article","created":{"date-parts":[[2007,11,14]],"date-time":"2007-11-14T21:04:56Z","timestamp":1195074296000},"page":"95-100","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":55,"title":["Leucine Aminopeptidase Is an Immunodominant Antigen of\n                    <i>Fasciola hepatica<\/i>\n                    Excretory and Secretory Products in Human Infections"],"prefix":"10.1128","volume":"15","author":[{"given":"A.","family":"Marcilla","sequence":"first","affiliation":[{"name":"A\u0301rea de Parasitologi\u0301a, Departamento de Biologi\u0301a Celular y Parasitologi\u0301a, Facultad de Farmacia, Universitat de Vale\u0300ncia, Av. Vicent Andres Estelles s\/n, 46100 Burjassot, Valencia, Spain"},{"name":"Departamento de Bioqui\u0301mica y Biologi\u0301a Molecular, Facultat de Cie\u0300ncies Biolo\u0300giques, Universitat de Valencia, Valencia, Spain"},{"name":"Unidad de Biologi\u0301a Parasitaria, Departamento de Biologi\u0301a Celular y Molecular, Facultad de Ciencias, Instituto de Higiene, Av. A. Navarro 3051 CP11600, Montevideo, Uruguay"},{"name":"Divisio\u0301n de Parasitologi\u0301a, Facultad de Farmacia, Universidad Miguel Herna\u0301ndez, Alicante, Spain"},{"name":"Departamento de Gene\u0301tica, Facultad de Medicina, Universidad de la Repu\u0301blica, Av. Gral. Flores 2121 CP11800, Montevideo, Uruguay"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. E.","family":"De la Rubia","sequence":"additional","affiliation":[{"name":"A\u0301rea de Parasitologi\u0301a, Departamento de Biologi\u0301a Celular y Parasitologi\u0301a, Facultad de Farmacia, Universitat de Vale\u0300ncia, Av. Vicent Andres Estelles s\/n, 46100 Burjassot, Valencia, Spain"},{"name":"Departamento de Bioqui\u0301mica y Biologi\u0301a Molecular, Facultat de Cie\u0300ncies Biolo\u0300giques, Universitat de Valencia, Valencia, Spain"},{"name":"Unidad de Biologi\u0301a Parasitaria, Departamento de Biologi\u0301a Celular y Molecular, Facultad de Ciencias, Instituto de Higiene, Av. A. Navarro 3051 CP11600, Montevideo, Uruguay"},{"name":"Divisio\u0301n de Parasitologi\u0301a, Facultad de Farmacia, Universidad Miguel Herna\u0301ndez, Alicante, Spain"},{"name":"Departamento de Gene\u0301tica, Facultad de Medicina, Universidad de la Repu\u0301blica, Av. Gral. Flores 2121 CP11800, Montevideo, Uruguay"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Sotillo","sequence":"additional","affiliation":[{"name":"A\u0301rea de Parasitologi\u0301a, Departamento de Biologi\u0301a Celular y Parasitologi\u0301a, Facultad de Farmacia, Universitat de Vale\u0300ncia, Av. Vicent Andres Estelles s\/n, 46100 Burjassot, Valencia, Spain"},{"name":"Departamento de Bioqui\u0301mica y Biologi\u0301a Molecular, Facultat de Cie\u0300ncies Biolo\u0300giques, Universitat de Valencia, Valencia, Spain"},{"name":"Unidad de Biologi\u0301a Parasitaria, Departamento de Biologi\u0301a Celular y Molecular, Facultad de Ciencias, Instituto de Higiene, Av. A. Navarro 3051 CP11600, Montevideo, Uruguay"},{"name":"Divisio\u0301n de Parasitologi\u0301a, Facultad de Farmacia, Universidad Miguel Herna\u0301ndez, Alicante, Spain"},{"name":"Departamento de Gene\u0301tica, Facultad de Medicina, Universidad de la Repu\u0301blica, Av. Gral. Flores 2121 CP11800, Montevideo, Uruguay"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.","family":"Bernal","sequence":"additional","affiliation":[{"name":"A\u0301rea de Parasitologi\u0301a, Departamento de Biologi\u0301a Celular y Parasitologi\u0301a, Facultad de Farmacia, Universitat de Vale\u0300ncia, Av. Vicent Andres Estelles s\/n, 46100 Burjassot, Valencia, Spain"},{"name":"Departamento de Bioqui\u0301mica y Biologi\u0301a Molecular, Facultat de Cie\u0300ncies Biolo\u0300giques, Universitat de Valencia, Valencia, Spain"},{"name":"Unidad de Biologi\u0301a Parasitaria, Departamento de Biologi\u0301a Celular y Molecular, Facultad de Ciencias, Instituto de Higiene, Av. A. Navarro 3051 CP11600, Montevideo, Uruguay"},{"name":"Divisio\u0301n de Parasitologi\u0301a, Facultad de Farmacia, Universidad Miguel Herna\u0301ndez, Alicante, Spain"},{"name":"Departamento de Gene\u0301tica, Facultad de Medicina, Universidad de la Repu\u0301blica, Av. Gral. Flores 2121 CP11800, Montevideo, Uruguay"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Carmona","sequence":"additional","affiliation":[{"name":"A\u0301rea de Parasitologi\u0301a, Departamento de Biologi\u0301a Celular y Parasitologi\u0301a, Facultad de Farmacia, Universitat de Vale\u0300ncia, Av. Vicent Andres Estelles s\/n, 46100 Burjassot, Valencia, Spain"},{"name":"Departamento de Bioqui\u0301mica y Biologi\u0301a Molecular, Facultat de Cie\u0300ncies Biolo\u0300giques, Universitat de Valencia, Valencia, Spain"},{"name":"Unidad de Biologi\u0301a Parasitaria, Departamento de Biologi\u0301a Celular y Molecular, Facultad de Ciencias, Instituto de Higiene, Av. A. Navarro 3051 CP11600, Montevideo, Uruguay"},{"name":"Divisio\u0301n de Parasitologi\u0301a, Facultad de Farmacia, Universidad Miguel Herna\u0301ndez, Alicante, Spain"},{"name":"Departamento de Gene\u0301tica, Facultad de Medicina, Universidad de la Repu\u0301blica, Av. Gral. Flores 2121 CP11800, Montevideo, Uruguay"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Z.","family":"Villavicencio","sequence":"additional","affiliation":[{"name":"A\u0301rea de Parasitologi\u0301a, Departamento de Biologi\u0301a Celular y Parasitologi\u0301a, Facultad de Farmacia, Universitat de Vale\u0300ncia, Av. Vicent Andres Estelles s\/n, 46100 Burjassot, Valencia, Spain"},{"name":"Departamento de Bioqui\u0301mica y Biologi\u0301a Molecular, Facultat de Cie\u0300ncies Biolo\u0300giques, Universitat de Valencia, Valencia, Spain"},{"name":"Unidad de Biologi\u0301a Parasitaria, Departamento de Biologi\u0301a Celular y Molecular, Facultad de Ciencias, Instituto de Higiene, Av. A. Navarro 3051 CP11600, Montevideo, Uruguay"},{"name":"Divisio\u0301n de Parasitologi\u0301a, Facultad de Farmacia, Universidad Miguel Herna\u0301ndez, Alicante, Spain"},{"name":"Departamento de Gene\u0301tica, Facultad de Medicina, Universidad de la Repu\u0301blica, Av. Gral. Flores 2121 CP11800, Montevideo, Uruguay"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.","family":"Acosta","sequence":"additional","affiliation":[{"name":"A\u0301rea de Parasitologi\u0301a, Departamento de Biologi\u0301a Celular y Parasitologi\u0301a, Facultad de Farmacia, Universitat de Vale\u0300ncia, Av. Vicent Andres Estelles s\/n, 46100 Burjassot, Valencia, Spain"},{"name":"Departamento de Bioqui\u0301mica y Biologi\u0301a Molecular, Facultat de Cie\u0300ncies Biolo\u0300giques, Universitat de Valencia, Valencia, Spain"},{"name":"Unidad de Biologi\u0301a Parasitaria, Departamento de Biologi\u0301a Celular y Molecular, Facultad de Ciencias, Instituto de Higiene, Av. A. Navarro 3051 CP11600, Montevideo, Uruguay"},{"name":"Divisio\u0301n de Parasitologi\u0301a, Facultad de Farmacia, Universidad Miguel Herna\u0301ndez, Alicante, Spain"},{"name":"Departamento de Gene\u0301tica, Facultad de Medicina, Universidad de la Repu\u0301blica, Av. Gral. Flores 2121 CP11800, Montevideo, Uruguay"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Tort","sequence":"additional","affiliation":[{"name":"A\u0301rea de Parasitologi\u0301a, Departamento de Biologi\u0301a Celular y Parasitologi\u0301a, Facultad de Farmacia, Universitat de Vale\u0300ncia, Av. Vicent Andres Estelles s\/n, 46100 Burjassot, Valencia, Spain"},{"name":"Departamento de Bioqui\u0301mica y Biologi\u0301a Molecular, Facultat de Cie\u0300ncies Biolo\u0300giques, Universitat de Valencia, Valencia, Spain"},{"name":"Unidad de Biologi\u0301a Parasitaria, Departamento de Biologi\u0301a Celular y Molecular, Facultad de Ciencias, Instituto de Higiene, Av. A. Navarro 3051 CP11600, Montevideo, Uruguay"},{"name":"Divisio\u0301n de Parasitologi\u0301a, Facultad de Farmacia, Universidad Miguel Herna\u0301ndez, Alicante, Spain"},{"name":"Departamento de Gene\u0301tica, Facultad de Medicina, Universidad de la Repu\u0301blica, Av. Gral. Flores 2121 CP11800, Montevideo, Uruguay"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F. J.","family":"Bornay","sequence":"additional","affiliation":[{"name":"A\u0301rea de Parasitologi\u0301a, Departamento de Biologi\u0301a Celular y Parasitologi\u0301a, Facultad de Farmacia, Universitat de Vale\u0300ncia, Av. Vicent Andres Estelles s\/n, 46100 Burjassot, Valencia, Spain"},{"name":"Departamento de Bioqui\u0301mica y Biologi\u0301a Molecular, Facultat de Cie\u0300ncies Biolo\u0300giques, Universitat de Valencia, Valencia, Spain"},{"name":"Unidad de Biologi\u0301a Parasitaria, Departamento de Biologi\u0301a Celular y Molecular, Facultad de Ciencias, Instituto de Higiene, Av. A. Navarro 3051 CP11600, Montevideo, Uruguay"},{"name":"Divisio\u0301n de Parasitologi\u0301a, Facultad de Farmacia, Universidad Miguel Herna\u0301ndez, Alicante, Spain"},{"name":"Departamento de Gene\u0301tica, Facultad de Medicina, Universidad de la Repu\u0301blica, Av. Gral. Flores 2121 CP11800, Montevideo, Uruguay"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. G.","family":"Esteban","sequence":"additional","affiliation":[{"name":"A\u0301rea de Parasitologi\u0301a, Departamento de Biologi\u0301a Celular y Parasitologi\u0301a, Facultad de Farmacia, Universitat de Vale\u0300ncia, Av. Vicent Andres Estelles s\/n, 46100 Burjassot, Valencia, Spain"},{"name":"Departamento de Bioqui\u0301mica y Biologi\u0301a Molecular, Facultat de Cie\u0300ncies Biolo\u0300giques, Universitat de Valencia, Valencia, Spain"},{"name":"Unidad de Biologi\u0301a Parasitaria, Departamento de Biologi\u0301a Celular y Molecular, Facultad de Ciencias, Instituto de Higiene, Av. A. Navarro 3051 CP11600, Montevideo, Uruguay"},{"name":"Divisio\u0301n de Parasitologi\u0301a, Facultad de Farmacia, Universidad Miguel Herna\u0301ndez, Alicante, Spain"},{"name":"Departamento de Gene\u0301tica, Facultad de Medicina, Universidad de la Repu\u0301blica, Av. Gral. Flores 2121 CP11800, Montevideo, Uruguay"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Toledo","sequence":"additional","affiliation":[{"name":"A\u0301rea de Parasitologi\u0301a, Departamento de Biologi\u0301a Celular y Parasitologi\u0301a, Facultad de Farmacia, Universitat de Vale\u0300ncia, Av. Vicent Andres Estelles s\/n, 46100 Burjassot, Valencia, Spain"},{"name":"Departamento de Bioqui\u0301mica y Biologi\u0301a Molecular, Facultat de Cie\u0300ncies Biolo\u0300giques, Universitat de Valencia, Valencia, Spain"},{"name":"Unidad de Biologi\u0301a Parasitaria, Departamento de Biologi\u0301a Celular y Molecular, Facultad de Ciencias, Instituto de Higiene, Av. A. Navarro 3051 CP11600, Montevideo, Uruguay"},{"name":"Divisio\u0301n de Parasitologi\u0301a, Facultad de Farmacia, Universidad Miguel Herna\u0301ndez, Alicante, Spain"},{"name":"Departamento de Gene\u0301tica, Facultad de Medicina, Universidad de la Repu\u0301blica, Av. Gral. Flores 2121 CP11800, Montevideo, Uruguay"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"1","DOI":"10.2307\/3284518","volume":"84","year":"1998","unstructured":"Acosta, D., F. Go\u00f1i, and C. Carmona. 1998. 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