{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T13:15:42Z","timestamp":1789996542518,"version":"4.0.1"},"reference-count":22,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2010,1,1]],"date-time":"2010-01-01T00:00:00Z","timestamp":1262304000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Vaccine Immunol"],"published-print":{"date-parts":[[2010,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            One of the best-characterized tests for the diagnosis of neurocysticercosis is the enzyme-linked immunoelectrotransfer blot assay, developed at the CDC, which uses lentil lectin-purified glycoproteins (LLGPs) extracted from\n            <jats:italic>Taenia solium<\/jats:italic>\n            cysticerci. The purification of the LLGP antigens has been difficult to standardize, and the polyacrylamide gel system used for the immunoblot assay is not easily transferable to other laboratories. In this study, we developed a multiantigen printing immunoassay (MAPIA) to compare the performance of multiple recombinant\n            <jats:italic>Taenia solium<\/jats:italic>\n            proteins with the potential for the detection of cysticercosis and taeniasis. We prepared MAPIA strips using six cysticercosis and two taeniasis diagnostic proteins and compared the performance of the proteins with sera collected from defined cysticercosis and taeniasis cases. Of the six cysticercosis antigens, rT24H performed well in detecting cases with two or more viable cysts in the brain (sensitivity and specificity, 97% and 99.4%, respectively); the use of a combination of cysticercosis antigens did not improve the sensitivity of the test and decreased the specificity. None of the antigens could differentiate the different clinical presentations of cysticercosis. Both of the taeniasis antigens (rES33 and rES38) had the same sensitivity of 99.4% and specificities of 93.9% and 94.5%, respectively. Some cross-reactivity against rES33 and rES38 was found, especially with sera from cases infected with\n            <jats:italic>Schistosoma mansoni<\/jats:italic>\n            . We conclude that MAPIA is a simple and effective tool that may be used to compare antibody responses to different cysticercosis and taeniasis antigens and, in this case, may be useful for the rapid detection of\n            <jats:italic>T. solium<\/jats:italic>\n            cases.\n          <\/jats:p>","DOI":"10.1128\/cvi.00339-09","type":"journal-article","created":{"date-parts":[[2009,11,12]],"date-time":"2009-11-12T03:17:06Z","timestamp":1257995826000},"page":"68-72","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":53,"title":["Multiantigen Print Immunoassay for Comparison of Diagnostic Antigens for\n            <i>Taenia solium<\/i>\n            Cysticercosis and Taeniasis"],"prefix":"10.1128","volume":"17","author":[{"given":"Sukwan","family":"Handali","sequence":"first","affiliation":[{"name":"Division of Parasitic Diseases, Coordinating Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Molly","family":"Klarman","sequence":"additional","affiliation":[{"name":"Division of Parasitic Diseases, Coordinating Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amanda N.","family":"Gaspard","sequence":"additional","affiliation":[{"name":"Division of Parasitic Diseases, Coordinating Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John","family":"Noh","sequence":"additional","affiliation":[{"name":"Division of Parasitic Diseases, Coordinating Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yeuk-Mui","family":"Lee","sequence":"additional","affiliation":[{"name":"Division of Parasitic Diseases, Coordinating Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvia","family":"Rodriguez","sequence":"additional","affiliation":[{"name":"Cysticercosis Unit, Instituto de Ciencias Neurologicas, Lima, Peru"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Armando E.","family":"Gonzalez","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine, Universidad Nacional Mayor de San Marcos, Lima, Peru"},{"name":"Bloomberg School of Public Health, Johns Hopkins University, Baltimore, Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hector H.","family":"Garcia","sequence":"additional","affiliation":[{"name":"Cysticercosis Unit, Instituto de Ciencias Neurologicas, Lima, Peru"},{"name":"Bloomberg School of Public Health, Johns Hopkins University, Baltimore, Maryland"},{"name":"Department of Microbiology and Center for Global Health, Universidad Peruana Cayetano Heredia, Lima, Peru"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert H.","family":"Gilman","sequence":"additional","affiliation":[{"name":"Bloomberg School of Public Health, Johns Hopkins University, Baltimore, Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Victor C. W.","family":"Tsang","sequence":"additional","affiliation":[{"name":"Department of Biology, Georgia State University, Atlanta, Georgia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricia P.","family":"Wilkins","sequence":"additional","affiliation":[{"name":"Division of Parasitic Diseases, Coordinating Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"416","DOI":"10.1177\/088307380201700604","volume":"17","year":"2002","unstructured":"Aguilar-Rebolledo, F., A. Meza-Lucas, J. Torres, R. Cedillo-Rivera, A. Enciso, R. C. Garcia, O. Munoz, and D. Correa. 2002. Evaluation of the enzyme-linked immunoelectrotransfer blot assay for diagnosis of neurocysticercosis in children. J. Child Neurol.17:416-420.","journal-title":"J. Child Neurol."},{"key":"e_1_3_2_3_2","unstructured":"Practical statistics for medical research. 1993"},{"key":"e_1_3_2_4_2","doi-asserted-by":"crossref","first-page":"278","DOI":"10.4269\/ajtmh.2005.72.278","volume":"72","year":"2005","unstructured":"Bueno, E. C., C. M. Scheel, A. J. Vaz, L. R. Machado, J. A. Livramento, O. M. Takayanagui, V. C. Tsang, and K. Hancock. 2005. Application of synthetic 8-kD and recombinant GP50 antigens in the diagnosis of neurocysticercosis by enzyme-linked immunosorbent assay. Am. J. Trop. Med. Hyg.72:278-283.","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"e_1_3_2_5_2","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1212\/WNL.57.2.177","volume":"57","year":"2001","unstructured":"Del Brutto, O. H., V. Rajshekhar, A. C. White, Jr., V. C. Tsang, T. E. Nash, O. M. Takayanagui, P. M. Schantz, C. A. Evans, A. Flisser, D. Correa, D. Botero, J. C. Allan, E. Sarti, A. E. Gonzalez, R. H. Gilman, and H. H. Garcia. 2001. 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