{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,23]],"date-time":"2026-04-23T06:03:26Z","timestamp":1776924206400,"version":"3.51.2"},"reference-count":24,"publisher":"American Society for Microbiology","issue":"10","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clin. Vaccine Immunol."],"published-print":{"date-parts":[[2013,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Infection with<jats:named-content content-type=\"genus-species\">Vibrio cholerae<\/jats:named-content>and oral cholera vaccines (OCVs) induce transient circulating plasmablast responses that peak within approximately 7 days after infection or vaccination. We previously demonstrated that plasmablast responses strongly correlate with subsequent levels of<jats:named-content content-type=\"genus-species\">V. cholerae<\/jats:named-content>-specific duodenal antibodies up to 6 months after<jats:named-content content-type=\"genus-species\">V. cholerae<\/jats:named-content>infection. Hence, plasmablast responses provide an early window into the immunologic memory at the mucosal surface. In this study, we characterized plasmablast responses following<jats:named-content content-type=\"genus-species\">V. cholerae<\/jats:named-content>infection using a flow cytometrically defined population and compared<jats:named-content content-type=\"genus-species\">V. cholerae<\/jats:named-content>-specific responses in adult patients with<jats:named-content content-type=\"genus-species\">V. cholerae<\/jats:named-content>O1 infection and vaccinees who received the OCV Dukoral (Crucell Vaccines Canada). Among flow cytometrically sorted populations of gut-homing plasmablasts, almost 50% of the cells recognized either cholera toxin B subunit (CtxB) or<jats:named-content content-type=\"genus-species\">V. cholerae<\/jats:named-content>O1 lipopolysaccharide (LPS). Using a traditional enzyme-linked immunosorbent spot assay (ELISPOT), we found that infection with<jats:named-content content-type=\"genus-species\">V. cholerae<\/jats:named-content>O1 and OCVs induce similar responses to the protein antigen CtxB, but responses to LPS were diminished after OCV compared to those after natural<jats:named-content content-type=\"genus-species\">V. cholerae<\/jats:named-content>infection. A second dose of OCV on day 14 failed to boost circulating<jats:named-content content-type=\"genus-species\">V. cholerae<\/jats:named-content>-specific plasmablast responses in Bangladeshi adults. Our results differ from those in studies from areas where cholera is not endemic, in which a second vaccination on day 14 significantly boosts plasmablast responses. Given these results, it is likely that the optimal boosting strategies for OCVs differ significantly between areas where<jats:named-content content-type=\"genus-species\">V. cholerae<\/jats:named-content>infection is endemic and those where it is not.<\/jats:p>","DOI":"10.1128\/cvi.00347-13","type":"journal-article","created":{"date-parts":[[2013,8,15]],"date-time":"2013-08-15T13:10:35Z","timestamp":1376572235000},"page":"1592-1598","source":"Crossref","is-referenced-by-count":30,"title":["Antibody-Secreting Cell Responses after Vibrio cholerae O1 Infection and Oral Cholera Vaccination in Adults in Bangladesh"],"prefix":"10.1128","volume":"20","author":[{"given":"Atiqur","family":"Rahman","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rasheduzzaman","family":"Rashu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Taufiqur Rahman","family":"Bhuiyan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fahima","family":"Chowdhury","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ashraful Islam","family":"Khan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kamrul","family":"Islam","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Regina C.","family":"LaRocque","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edward T.","family":"Ryan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jens","family":"Wrammert","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephen B.","family":"Calderwood","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Firdausi","family":"Qadri","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jason B.","family":"Harris","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","published-online":{"date-parts":[[2013,8,14]]},"reference":[{"key":"key20180919073710_B1","first-page":"117","article-title":"Cholera vaccines: WHO position paper","volume":"85","author":"World Health Organization","year":"2010","journal-title":"Wkly. Epidemiol. Rec."},{"key":"key20180919073710_B2","doi-asserted-by":"crossref","first-page":"1961","DOI":"10.1016\/S0140-6736(10)62172-1","article-title":"Cholera's western front","volume":"376","author":"Harris","year":"2010","journal-title":"Lancet"},{"key":"key20180919073710_B3","doi-asserted-by":"publisher","first-page":"e1003","DOI":"10.1371\/journal.pntd.0001003","article-title":"The cholera pandemic, still with us after half a century: time to rethink","volume":"5","author":"Ryan","year":"2011","journal-title":"PLoS Negl. Trop. 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Epidemiol."},{"key":"key20180919073710_B7","doi-asserted-by":"crossref","first-page":"696","DOI":"10.1038\/nature03820","article-title":"Refractory periods and climate forcing in cholera dynamics","volume":"436","author":"Koelle","year":"2005","journal-title":"Nature"},{"key":"key20180919073710_B8","doi-asserted-by":"crossref","first-page":"818","DOI":"10.1093\/infdis\/143.6.818","article-title":"Duration of infection-derived immunity to cholera","volume":"143","author":"Levine","year":"1981","journal-title":"J. Infect. 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Vaccine Immunol."},{"key":"key20180919073710_B17","doi-asserted-by":"crossref","first-page":"1386","DOI":"10.1016\/j.vaccine.2008.12.041","article-title":"Robust gut associated vaccine-specific antibody-secreting cell responses are detected at the mucosal surface of Bangladeshi subjects after immunization with an oral killed bivalent V. cholerae O1\/O139 whole cell cholera vaccine: comparison with other mucosal and systemic responses","volume":"27","author":"Shamsuzzaman","year":"2009","journal-title":"Vaccine"},{"key":"key20180919073710_B18","doi-asserted-by":"crossref","first-page":"1055","DOI":"10.1086\/591500","article-title":"Cholera toxin-specific memory B cell responses are induced in patients with dehydrating diarrhea caused by Vibrio cholerae O1","volume":"198","author":"Jayasekera","year":"2008","journal-title":"J. Infect. 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