{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T14:35:17Z","timestamp":1790519717648,"version":"4.1.0"},"reference-count":17,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2009,1,1]],"date-time":"2009-01-01T00:00:00Z","timestamp":1230768000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Vaccine Immunol"],"published-print":{"date-parts":[[2009,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The human herpes simplex virus (HSV) is highly pathogenic, with infections caused by two distinct antigenic types, HSV-1 and HSV-2. Differentiation of antibodies to these specific antigens can provide useful information for the diagnosis of subclinical or undiagnosed HSV-2 infections, as well as for reducing the risk of maternal transfer of HSV to the neonate. In this study, a multiplex assay capable of concurrent detection of HSV-1 and -2 immunoglobulin G (IgG) antibodies was compared to immunoblot, Western blot, and enzyme-linked immunosorbent assays. Agreement of the multiplex assay was 95% or greater (\n            <jats:italic>n<\/jats:italic>\n            = 332) for both HSV-1 and -2 compared to the three assays. Sensitivities for HSV-1 ranged from 94.9 to 97.9%, with specificities of 93 to 97%. For HSV-2, the sensitivity and specificity ranges were 92.6 to 98.9% and 98.3 to 98.7%, respectively. Our studies show that the multiplexed microsphere-based assay offers a sensitive and specific alternative method for the detection HSV-1 and -2 type-specific antibodies. Advantages of the multiplex assay include multiple results per assay, the inclusion of internal controls for each specimen, and higher throughput of results.\n          <\/jats:p>","DOI":"10.1128\/cvi.00351-08","type":"journal-article","created":{"date-parts":[[2008,11,20]],"date-time":"2008-11-20T01:44:24Z","timestamp":1227145464000},"page":"55-60","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":15,"title":["Comparison of a Multiplexed Herpes Simplex Virus Type-Specific Immunoglobulin G Serology Assay to Immunoblot, Western Blot, and Enzyme-Linked Immunosorbent Assays"],"prefix":"10.1128","volume":"16","author":[{"given":"Thomas B.","family":"Martins","sequence":"first","affiliation":[{"name":"Associated Regional and University Pathologists Institute for Clinical and Experimental Pathology, Salt Lake City, Utah"},{"name":"Departments of Pathology"},{"name":"Pediatrics"},{"name":"Medicine, University of Utah School of Medicine, Salt Lake City, Utah"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryan J.","family":"Welch","sequence":"additional","affiliation":[{"name":"Associated Regional and University Pathologists Institute for Clinical and Experimental Pathology, Salt Lake City, Utah"},{"name":"Departments of Pathology"},{"name":"Pediatrics"},{"name":"Medicine, University of Utah School of Medicine, Salt Lake City, Utah"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Harry R.","family":"Hill","sequence":"additional","affiliation":[{"name":"Associated Regional and University Pathologists Institute for Clinical and Experimental Pathology, Salt Lake City, Utah"},{"name":"Departments of Pathology"},{"name":"Pediatrics"},{"name":"Medicine, University of Utah School of Medicine, Salt Lake City, Utah"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christine M.","family":"Litwin","sequence":"additional","affiliation":[{"name":"Associated Regional and University Pathologists Institute for Clinical and Experimental Pathology, Salt Lake City, Utah"},{"name":"Departments of Pathology"},{"name":"Pediatrics"},{"name":"Medicine, University of Utah School of Medicine, Salt Lake City, Utah"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_1_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.26.4.662-667.1988"},{"key":"e_1_3_1_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.12.1.1"},{"key":"e_1_3_1_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.36.1.294-295.1998"},{"key":"e_1_3_1_5_2","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1016\/S1386-6532(02)00105-1","volume":"27","year":"2003","unstructured":"Coyle, P. V., H. J. O'Neill, D. E. Wyatt, C. McCaughey, S. Quah, and M. O. McBride. 2003. Emergence of herpes simplex type 1 as the main cause of recurrent genital ulcerative disease in women in Northern Ireland. J. Clin. Virol.27:22-29.","journal-title":"J. Clin. Virol."},{"key":"e_1_3_1_6_2","unstructured":"Statistical methods for rates and proportions 1981"},{"key":"e_1_3_1_7_2","doi-asserted-by":"crossref","first-page":"2127","DOI":"10.1016\/S0140-6736(07)61908-4","volume":"370","year":"2007","unstructured":"Gupta, R., T. Warren, and A. Wald. 2007. Genital herpes. Lancet370:2127-2137.","journal-title":"Lancet"},{"key":"e_1_3_1_8_2","first-page":"65A","volume":"11","year":"2004","unstructured":"Kimberlin, D. 2004. Herpes simplex virus, meningitis and encephalitis in neonates. Herpes11(Suppl. 2):65A-76A.","journal-title":"Herpes"},{"key":"e_1_3_1_9_2","doi-asserted-by":"crossref","first-page":"1454","DOI":"10.1086\/315395","volume":"181","year":"2000","unstructured":"Lafferty, W. E., L. Downey, C. Celum, and A. Wald. 2000. Herpes simplex virus type 1 as a cause of genital herpes: impact on surveillance and prevention. J. Infect. Dis.181:1454-1457.","journal-title":"J. Infect. Dis."},{"key":"e_1_3_1_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.37.8.2717-2718.1999"},{"key":"e_1_3_1_11_2","doi-asserted-by":"crossref","first-page":"272","DOI":"10.1309\/RV29-7CCN-5DYW-M4Y6","volume":"115","year":"2001","unstructured":"Martins, T. B., R. D. Woolstenhulme, T. D. Jaskowski, H. R. Hill, and C. M. Litwin. 2001. Comparison of four enzyme immunoassays with a Western blot assay for the determination of type-specific antibodies to herpes simplex virus. Am. J. Clin. Pathol.115:272-277.","journal-title":"Am. J. Clin. Pathol."},{"key":"e_1_3_1_12_2","doi-asserted-by":"crossref","first-page":"801","DOI":"10.1097\/01.OLQ.0000093080.55201.D1","volume":"30","year":"2003","unstructured":"Mertz, G. J., S. L. Rosenthal, and L. R. Stanberry. 2003. Is herpes simplex virus type 1 (HSV-1) now more common than HSV-2 in first episodes of genital herpes? Sex. Transm. Dis.30:801-802.","journal-title":"Sex. Transm. Dis."},{"key":"e_1_3_1_13_2","doi-asserted-by":"crossref","first-page":"463","DOI":"10.1111\/j.1469-0691.2006.01370.x","volume":"12","year":"2006","unstructured":"Morrow, R., and D. Friedrich. 2006. Performance of a novel test for IgM and IgG antibodies in subjects with culture-documented genital herpes simplex virus-1 or -2 infection. Clin. Microbiol. Infect.12:463-469.","journal-title":"Clin. Microbiol. Infect."},{"key":"e_1_3_1_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00144-07"},{"key":"e_1_3_1_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.9.3321-3325.2001"},{"key":"e_1_3_1_16_2","doi-asserted-by":"crossref","first-page":"138","DOI":"10.1016\/j.jviromet.2007.04.007","volume":"144","year":"2007","unstructured":"Sauerbrei, A., and P. Wutzler. 2007. Novel recombinant ELISA assays for determination of type-specific IgG antibodies against HSV-1 and HSV-2. J. Virol. Methods144:138-142.","journal-title":"J. Virol. Methods"},{"key":"e_1_3_1_17_2","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1097\/00007435-200102000-00007","volume":"28","year":"2001","unstructured":"Whittington, W. L., C. L. Celum, A. Cent, and R. L. Ashley. 2001. Use of a glycoprotein G-based type-specific assay to detect antibodies to herpes simplex virus type 2 among persons attending sexually transmitted disease clinics. Sex. Transm. Dis.28:99-104.","journal-title":"Sex. Transm. Dis."},{"key":"e_1_3_1_18_2","doi-asserted-by":"crossref","first-page":"964","DOI":"10.1001\/jama.296.8.964","volume":"296","year":"2006","unstructured":"Xu, F., M. R. Sternberg, B. J. Kottiri, G. M. McQuillan, F. K. Lee, A. J. Nahmias, S. M. Berman, and L. E. Markowitz. 2006. Trends in herpes simplex virus type 1 and type 2 seroprevalence in the United States. 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