{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,7]],"date-time":"2025-11-07T13:20:09Z","timestamp":1762521609120},"reference-count":22,"publisher":"American Society for Microbiology","issue":"10","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clin. Vaccine Immunol."],"published-print":{"date-parts":[[2013,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p><jats:named-content content-type=\"genus-species\">Neospora caninum<\/jats:named-content>is an apicomplexan parasite that causes abortion in cattle; hence, accurate diagnosis of this pathogen is important to the cattle farming industry. Our previous proteomics and immunoscreening analyses revealed that the<jats:named-content content-type=\"genus-species\">N. caninum<\/jats:named-content>subtilisin-like serine protease 1 (NcSUB1) has potential as a serodiagnostic tool for<jats:named-content content-type=\"genus-species\">Neospora<\/jats:named-content>. Consequently, we expressed two fragments containing five NcSUB1 tandem repeat copies covering amino acids (aa) 524 to 843 (NcSUB1t) and 555 to 679 (NcSUB1tr) to identify the antigenic regions. The serodiagnostic performances of NcSUB1t and NcSUB1tr were compared with that of N54, which contains a single copy of the repeats (aa 649 to 784), and with the truncated NcSAG1 (NcSAG1t), which lacks a signal peptide and C-terminal hydrophobic regions, as a positive reference. Serum samples from<jats:named-content content-type=\"genus-species\">N. caninum<\/jats:named-content>experimentally infected cattle and mice and cattle from a farm with confirmed cases of<jats:named-content content-type=\"genus-species\">Neospora<\/jats:named-content>abortion were tested by enzyme-linked immunosorbent assay (ELISA) with the four antigens. In the<jats:named-content content-type=\"genus-species\">N. caninum<\/jats:named-content>experimentally infected cattle, the highest IgG1 antibody titers were detected against NcSUB1t, while specific IgG1 antibodies were detectable from 16 days postinfection (dpi), with levels peaking at 36 dpi for all of the antigens. On the other hand, the levels of anti-NcSUB1 IgG2 antibodies were lower than those of anti-SAG1t IgG2 antibodies. The ELISA with NcSUB1t and NcSUB1tr had good sensitivity (94.59 to 95.95%) and specificity (80 to 100%) with bovine serum field samples compared to NcSAG1t and showed no cross-reactions with sera from<jats:named-content content-type=\"genus-species\">Toxoplasma gondii<\/jats:named-content>experimentally infected mice. Moreover, IgG antibodies against NcSUB1t were detected during parturition in the NcSAG1t antibody-positive cattle, and NcSUB1t-specific antibody transfer was observed from a mother to her calf. Our results show that the NcSUB1 tandem repeat is potentially useful for serodiagnosis of<jats:named-content content-type=\"genus-species\">N. caninum<\/jats:named-content>.<\/jats:p>","DOI":"10.1128\/cvi.00352-13","type":"journal-article","created":{"date-parts":[[2013,8,22]],"date-time":"2013-08-22T01:42:51Z","timestamp":1377135771000},"page":"1617-1622","source":"Crossref","is-referenced-by-count":12,"title":["Identification of a Highly Antigenic Region of Subtilisin-Like Serine Protease 1 for Serodiagnosis of Neospora caninum Infection"],"prefix":"10.1128","volume":"20","author":[{"given":"Rochelle Haidee D.","family":"Yba\u00f1ez","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohamad Alaa","family":"Terkawi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kyohko","family":"Kameyama","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xuenan","family":"Xuan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshifumi","family":"Nishikawa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","published-online":{"date-parts":[[2013,8,21]]},"reference":[{"key":"key20180919073711_B1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0304-4017(96)01035-7","article-title":"A review of Neospora caninum and neosporosis","volume":"67","author":"Dubey","year":"1996","journal-title":"Vet. 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Parasitol."},{"key":"key20180919073711_B6","doi-asserted-by":"crossref","first-page":"473","DOI":"10.1016\/j.cvfa.2005.03.004","article-title":"Neosporosis in cattle","volume":"2","author":"Dubey","year":"2005","journal-title":"Vet. Clin. North Am. Food Anim. Pract."},{"key":"key20180919073711_B7","doi-asserted-by":"crossref","first-page":"1063","DOI":"10.2307\/3284363","article-title":"Antibody responses of cows during an outbreak of neosporosis evaluated by indirect fluorescent antibody test and different enzyme-linked immunosorbent assays","volume":"83","author":"Dubey","year":"1997","journal-title":"J. Parasitol."},{"key":"key20180919073711_B8","doi-asserted-by":"crossref","first-page":"631","DOI":"10.1016\/S0020-7519(01)00363-0","article-title":"Diagnosis and seroepidemiology of Neospora caninum-associated bovine abortion","volume":"32","author":"Jenkins","year":"2002","journal-title":"Int. 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