{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,10]],"date-time":"2026-01-10T03:52:14Z","timestamp":1768017134214,"version":"3.49.0"},"reference-count":29,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2009,1,1]],"date-time":"2009-01-01T00:00:00Z","timestamp":1230768000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Vaccine Immunol"],"published-print":{"date-parts":[[2009,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>In this study, the early pulmonary cytokine and chemokine responses in mice immunized with either BCG vaccine, a \u0394<jats:italic>secA2<\/jats:italic>mutant of<jats:italic>Mycobacterium tuberculosis<\/jats:italic>, or a DNA vaccine expressing an ESAT6-antigen 85B fusion protein and then aerogenically challenged with a low dose of<jats:italic>M. tuberculosis<\/jats:italic>were evaluated by PCR array. The cellular immune responses at day 10 postchallenge were essentially equivalent in the lungs of mice immunized with either the highly immunogenic BCG vaccine or the \u0394<jats:italic>secA2 M. tuberculosis<\/jats:italic>mutant strain. Specifically, 12 immune biomolecules (including gamma interferon [IFN-\u03b3], interleukin-21 [IL-21], IL-27, IL-17f, CXCL9, CXCL10, and CXCL11) were differentially regulated, relative to the levels for nai\u0308ve controls, in the lungs of vaccinated mice at this time point. Although the vaccine-related immune responses evoked in mice immunized with the DNA vaccine were relatively limited at 10 days postinfection, upregulation of IFN-\u03b3 RNA synthesis as well as increased expression levels of CXCL9, CXCL10, and CXCL11 chemokines were detected.<\/jats:p>","DOI":"10.1128\/cvi.00359-08","type":"journal-article","created":{"date-parts":[[2008,11,27]],"date-time":"2008-11-27T02:47:39Z","timestamp":1227754059000},"page":"122-126","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":30,"title":["Early Pulmonary Cytokine and Chemokine Responses in Mice Immunized with Three Different Vaccines against<i>Mycobacterium tuberculosis<\/i>Determined by PCR Array"],"prefix":"10.1128","volume":"16","author":[{"given":"JaeHyun","family":"Lim","sequence":"first","affiliation":[{"name":"Laboratory of Mycobacterial Diseases and Cellular Immunology, Center for Biologics Evaluation and Research, United States Food and Drug Administration, Bethesda, Maryland"},{"name":"Department of Microbiology and Immunology and Department of Medicine, Albert Einstein College of Medicine, Bronx, New York"},{"name":"Department of Microbiology and Immunology, Department of Genetics, and the Howard Hughes Medical Institute, Albert Einstein College of Medicine, Bronx, New York"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Steven C.","family":"Derrick","sequence":"additional","affiliation":[{"name":"Laboratory of Mycobacterial Diseases and Cellular Immunology, Center for Biologics Evaluation and Research, United States Food and Drug Administration, Bethesda, Maryland"},{"name":"Department of Microbiology and Immunology and Department of Medicine, Albert Einstein College of Medicine, Bronx, New York"},{"name":"Department of Microbiology and Immunology, Department of Genetics, and the Howard Hughes Medical Institute, Albert Einstein College of Medicine, Bronx, New York"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kristopher","family":"Kolibab","sequence":"additional","affiliation":[{"name":"Laboratory of Mycobacterial Diseases and Cellular Immunology, Center for Biologics Evaluation and Research, United States Food and Drug Administration, Bethesda, Maryland"},{"name":"Department of Microbiology and Immunology and Department of Medicine, Albert Einstein College of Medicine, Bronx, New York"},{"name":"Department of Microbiology and Immunology, Department of Genetics, and the Howard Hughes Medical Institute, Albert Einstein College of Medicine, Bronx, New York"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amy Li","family":"Yang","sequence":"additional","affiliation":[{"name":"Laboratory of Mycobacterial Diseases and Cellular Immunology, Center for Biologics Evaluation and Research, United States Food and Drug Administration, Bethesda, Maryland"},{"name":"Department of Microbiology and Immunology and Department of Medicine, Albert Einstein College of Medicine, Bronx, New York"},{"name":"Department of Microbiology and Immunology, Department of Genetics, and the Howard Hughes Medical Institute, Albert Einstein College of Medicine, Bronx, New York"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Steven","family":"Porcelli","sequence":"additional","affiliation":[{"name":"Laboratory of Mycobacterial Diseases and Cellular Immunology, Center for Biologics Evaluation and Research, United States Food and Drug Administration, Bethesda, Maryland"},{"name":"Department of Microbiology and Immunology and Department of Medicine, Albert Einstein College of Medicine, Bronx, New York"},{"name":"Department of Microbiology and Immunology, Department of Genetics, and the Howard Hughes Medical Institute, Albert Einstein College of Medicine, Bronx, New York"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"William R.","family":"Jacobs","sequence":"additional","affiliation":[{"name":"Laboratory of Mycobacterial Diseases and Cellular Immunology, Center for Biologics Evaluation and Research, United States Food and Drug Administration, Bethesda, Maryland"},{"name":"Department of Microbiology and Immunology and Department of Medicine, Albert Einstein College of Medicine, Bronx, New York"},{"name":"Department of Microbiology and Immunology, Department of Genetics, and the Howard Hughes Medical Institute, Albert Einstein College of Medicine, Bronx, New York"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sheldon L.","family":"Morris","sequence":"additional","affiliation":[{"name":"Laboratory of Mycobacterial Diseases and Cellular Immunology, Center for Biologics Evaluation and Research, United States Food and Drug Administration, Bethesda, Maryland"},{"name":"Department of Microbiology and Immunology and Department of Medicine, Albert Einstein College of Medicine, Bronx, New York"},{"name":"Department of Microbiology and Immunology, Department of Genetics, and the Howard Hughes Medical Institute, Albert Einstein College of Medicine, Bronx, New York"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_1_2_2","doi-asserted-by":"crossref","first-page":"507","DOI":"10.1080\/01902140701756604","volume":"33","year":"2007","unstructured":"Aujla, S. J., P. J. Dubin, and J. K. Kolls. 2007. Interleukin-17 in pulmonary host defense. Exp. Lung Res.33:507-518.","journal-title":"Exp. Lung Res."},{"key":"e_1_3_1_3_2","doi-asserted-by":"crossref","first-page":"698","DOI":"10.1001\/jama.1994.03510330076038","volume":"271","year":"1994","unstructured":"Colditz, G. A., T. F. Brewer, C. S. Berkey, M. E. Wilson, E. Burdick, H. V. Fineberg, and F. Mosteller. 1994. Efficacy of BCG vaccine in the prevention of tuberculosis. Meta-analysis of the published literature. JAMA271:698-702.","journal-title":"JAMA"},{"key":"e_1_3_1_4_2","doi-asserted-by":"crossref","first-page":"623","DOI":"10.4049\/jimmunol.167.2.623","volume":"167","year":"2001","unstructured":"Cole, A. M., T. Ganz, A. M. Liese, M. D. Burdick, L. Liu, and R. M. Strieter. 2001. Cutting edge: IFN-inducible ELR-CXC chemokines display defensin-like antimicrobial activity. J. Immunol.167:623-627.","journal-title":"J. Immunol."},{"key":"e_1_3_1_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.1.292-302.2002"},{"key":"e_1_3_1_6_2","doi-asserted-by":"crossref","first-page":"780","DOI":"10.1016\/j.vaccine.2004.07.036","volume":"23","year":"2004","unstructured":"Derrick, S. C., A. L. Yang, and S. L. Morris. 2004. A polyvalent DNA vaccine expressing an ESAT6-Ag85B fusion protein protects mice against a primary infection with Mycobacterium tuberculosis and boosts BCG-induced protective immunity. Vaccine23:780-788.","journal-title":"Vaccine"},{"key":"e_1_3_1_7_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.19.1.93"},{"key":"e_1_3_1_8_2","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1016\/j.tube.2003.08.010","volume":"84","year":"2004","unstructured":"Flynn, J. L. 2004. Immunology of tuberculosis and implications in vaccine development. Tuberculosis (Edinburgh)84:93-101.","journal-title":"Tuberculosis (Edinburgh)"},{"key":"e_1_3_1_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.12.7023-7034.2003"},{"key":"e_1_3_1_10_2","doi-asserted-by":"crossref","first-page":"3522","DOI":"10.1016\/j.vaccine.2006.02.005","volume":"24","year":"2006","unstructured":"Goter-Robinson, C., S. C. Derrick, A. L. Yang, B. Y. Jeon, and S. L. Morris. 2006. Protection against an aerogenic Mycobacterium tuberculosis infection in BCG-immunized and DNA-vaccinated mice is associated with early type I cytokine responses. Vaccine24:3522-3529.","journal-title":"Vaccine"},{"key":"e_1_3_1_11_2","doi-asserted-by":"crossref","first-page":"7980","DOI":"10.4049\/jimmunol.177.11.7980","volume":"177","year":"2006","unstructured":"Higgins, S. C., A. G. Janecki, E. C. Lavelle, and K. H. Mills. 2006. TLR4 mediates vaccine-induced protective cellular immunity to Bordetella pertussis: role of IL-17-producing T cells. J. Immunol.177:7980-7989.","journal-title":"J. Immunol."},{"key":"e_1_3_1_12_2","doi-asserted-by":"crossref","first-page":"2279","DOI":"10.1172\/JCI31947","volume":"117","year":"2007","unstructured":"Hinchey, J., S. Lee, B. Y. Jeon, R. J. Basaraba, M. M. Venkataswamy, B. Chen, J. Chan, M. Braunstein, I. M. Orme, S. C. Derrick, S. L. Morris, W. R. Jacobs, Jr., and S. A. Porcelli. 2007. Enhanced priming of adaptive immunity by a proapoptotic mutant of Mycobacterium tuberculosis. J. Clin. Investig.117:2279-2288.","journal-title":"J. Clin. Investig."},{"key":"e_1_3_1_13_2","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1086\/509895","volume":"195","year":"2007","unstructured":"Huang, D., L. Qiu, R. Wang, X. Lai, G. Du, P. Seghal, Y. Shen, L. Shao, L. Halliday, J. Fortman, L. Shen, N. L. Letvin, and Z. W. Chen. 2007. Immune gene networks of mycobacterial vaccine-elicited cellular responses and immunity. J. Infect. Dis.195:55-69.","journal-title":"J. Infect. Dis."},{"key":"e_1_3_1_14_2","doi-asserted-by":"crossref","first-page":"521","DOI":"10.1038\/nri1648","volume":"5","year":"2005","unstructured":"Hunter, C. A. 2005. New IL-12-family members: IL-23 and IL-27, cytokines with divergent functions. Nat. Rev. Immunol.5:521-531.","journal-title":"Nat. Rev. Immunol."},{"key":"e_1_3_1_15_2","first-page":"369","volume":"8","year":"2007","unstructured":"Khader, S. A., G. K. Bell, J. E. Pearl, J. J. Fountain, J. Rangel-Moreno, G. E. Gilley, F. Shen, S. M. Eaton, S. I. Gaffen, S. L. Swain, R. M. Locksley, L. Haynes, T. D. Randall, and A. M. Cooper. 2007. IL-23 and IL-17 in the establishment of protective pulmonary CD4+ T cell responses after vaccination and during Mycobacterium tuberculosis challenge. Nat. Immunol.8:369-377.","journal-title":"Nat. Immunol."},{"key":"e_1_3_1_16_2","doi-asserted-by":"crossref","first-page":"484","DOI":"10.1038\/nature05970","volume":"448","year":"2007","unstructured":"Korn, T., E. Bettelli, W. Gao, J. Awasthi, A. J\u00e4ger, T. B. Strom, M. Oukka, and V. K. Kuchroo. 2007. IL-21 initiates an alternative pathway to induce proinflammatory T(H)17 cells. Nature448:484-487.","journal-title":"Nature"},{"key":"e_1_3_1_17_2","doi-asserted-by":"crossref","first-page":"2347","DOI":"10.4049\/jimmunol.162.4.2347","volume":"162","year":"1999","unstructured":"Laan, M., Z. H. Cui, H. Hoshino, J. Lotvall, M. Sjostrand, D. C. Gruenert, B. E. Skough, and A. Linde. 1999. Neutrophil recruitment by human IL-17 via C-X-C chemokine release in the airways. J. Immunol.162:2347-2352.","journal-title":"J. Immunol."},{"key":"e_1_3_1_18_2","doi-asserted-by":"crossref","first-page":"688","DOI":"10.1038\/nri1688","volume":"5","year":"2005","unstructured":"Leonard, W. J., and R. Spolski. 2005. Interleukin-21: a modulator of lymphoid proliferation, apoptosis and differentiation. Nat. Rev. Immunol.5:688-698.","journal-title":"Nat. Rev. Immunol."},{"key":"e_1_3_1_19_2","doi-asserted-by":"crossref","first-page":"1069","DOI":"10.4161\/cc.5.10.2771","volume":"5","year":"2006","unstructured":"Levine, A. J., and A. H. Brivanlou. 2006. GDF3 at the crossroads of TGF-beta signaling. Cell Cycle5:1069-1073.","journal-title":"Cell Cycle"},{"key":"e_1_3_1_20_2","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1089\/jir.2007.0100","volume":"28","year":"2008","unstructured":"Linge, H. M., M. Collin, A. Giwercman, J. Milan, A. Bjartell, and A. Egesten. 2008. The antibacterial chemokine MIG\/CXCL9 is constitutively expressed in epithelial cells of the male urogenital tract and is present in seminal plasma. J. Interferon Cytokine Res.28:191-196.","journal-title":"J. Interferon Cytokine Res."},{"key":"e_1_3_1_21_2","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1016\/j.micinf.2005.06.015","volume":"8","year":"2006","unstructured":"Mollenkopf, H. J., K. Hahnke, and S. H. E. Kaufmann. 2006. Transcriptional responses in mouse lungs induced by vaccination with Mycobacterium bovis BCG and infection with Mycobacterium tuberculosis. Microbes Infect.8:136-144.","journal-title":"Microbes Infect."},{"key":"e_1_3_1_22_2","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1016\/S0167-5699(98)01321-8","volume":"19","year":"1998","unstructured":"Ottenhoff, T. H., D. Kumararatne, and J. L. Casanova. 1998. Novel human immunodeficiencies reveal the essential role of type-I cytokines in immunity to intracellular bacteria. Immunol. Today19:491-494.","journal-title":"Immunol. Today"},{"key":"e_1_3_1_23_2","doi-asserted-by":"crossref","first-page":"7490","DOI":"10.4049\/jimmunol.173.12.7490","volume":"173","year":"2004","unstructured":"Pearl, J. E., S. A. Khader, A. Solache, L. Gilmartin, N. Ghilardi, F. deSauvage, and A. M. Cooper. 2004. IL-27 signaling compromises control of bacterial growth in Mycobacteria-infected mice. J. Immunol.173:7490-7496.","journal-title":"J. Immunol."},{"key":"e_1_3_1_24_2","doi-asserted-by":"crossref","first-page":"1503","DOI":"10.1016\/S0002-9440(10)64101-6","volume":"158","year":"2001","unstructured":"Qiu, B., K. A. Frait, F. Reich, E. Komuniecki, and S. W. Chensue. 2001. Chemokine expression dynamics in mycobacterial (type-1) and schistosomal (type-2) antigen-elicited pulmonary granuloma formation. Am. J. Pathol.158:1503-1515.","journal-title":"Am. J. Pathol."},{"key":"e_1_3_1_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.63.10.3871-3877.1995"},{"key":"e_1_3_1_26_2","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1016\/j.tube.2006.01.020","volume":"86","year":"2006","unstructured":"Rodgers, A., K. M. Whitmore, and K. B. Walker. 2006. Potential correlates of BCG induced protection against tuberculosis detected in a mouse aerosol model using gene expression profiling. Tuberculosis86:255-262.","journal-title":"Tuberculosis"},{"key":"e_1_3_1_27_2","doi-asserted-by":"crossref","first-page":"3569","DOI":"10.4049\/jimmunol.180.5.3569","volume":"180","year":"2008","unstructured":"Soares, A. P., T. J. Scriba, S. Joseph, R. Harbacheuski, R. A. Murray, S. J. Gelderbloem, A. Hawkridge, G. D. Hussey, H. Maecker, G. Kaplan, and W. A. Hanekom. 2008. Bacillus Calmette-Gu\u00e9rin vaccination of human newborns induces T cells with complex cytokine and phenotypic profiles. J. Immunol.180:3569-3577.","journal-title":"J. Immunol."},{"key":"e_1_3_1_28_2","doi-asserted-by":"crossref","first-page":"3689","DOI":"10.1002\/1521-4141(200012)30:12<3689::AID-IMMU3689>3.0.CO;2-4","volume":"30","year":"2000","unstructured":"van Pinxteren, L. A., J. P. Cassidy, B. H. Smedegaard, E. M. Agger, and P. Andersen. 2000. Control of latent Mycobacterium tuberculosis infection is dependent on CD8 T cells. Eur. J. Immunol.30:3689-3698.","journal-title":"Eur. J. Immunol."},{"key":"e_1_3_1_29_2","unstructured":"Global tuberculosis control\u2014surveillance planning financing 2008"},{"key":"e_1_3_1_30_2","doi-asserted-by":"crossref","first-page":"1255","DOI":"10.1172\/JCI34614","volume":"118","year":"2008","unstructured":"Young, D. B., M. D. Perkins, K. Duncan, and C. E. Barry III. 2008. Confronting the scientific obstacles to global control of tuberculosis. J. Clin. Investig.118:1255-1265.","journal-title":"J. Clin. Investig."}],"container-title":["Clinical and Vaccine Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/CVI.00359-08","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/CVI.00359-08","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,5,21]],"date-time":"2023-05-21T14:24:36Z","timestamp":1684679076000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/CVI.00359-08"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,1]]},"references-count":29,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2009,1]]}},"alternative-id":["10.1128\/CVI.00359-08"],"URL":"https:\/\/doi.org\/10.1128\/cvi.00359-08","relation":{},"ISSN":["1556-6811","1556-679X"],"issn-type":[{"value":"1556-6811","type":"print"},{"value":"1556-679X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,1]]},"assertion":[{"value":"2008-09-30","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2008-11-14","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-12-18","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}