{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T01:32:49Z","timestamp":1773797569077,"version":"3.50.1"},"reference-count":63,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2009,3,1]],"date-time":"2009-03-01T00:00:00Z","timestamp":1235865600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Vaccine Immunol"],"published-print":{"date-parts":[[2009,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p><jats:italic>Francisella tularensis<\/jats:italic>is the causative agent of tularemia and a potential agent of biowarfare. As an easily transmissible infectious agent, rapid detection and treatment are necessary to provide a positive clinical outcome. As an agent of biowarfare, there is an additional need to prevent infection. We made monoclonal antibodies to the<jats:italic>F. tularensis<\/jats:italic>subsp.<jats:italic>holarctica<\/jats:italic>live vaccine strain (<jats:italic>F. tularensis<\/jats:italic>LVS) by infecting mice with a sublethal dose of bacteria and, following recovery, by boosting the mice with sonicated organisms. The response to the initial and primary infection was restricted to immunoglobulin M antibody directed solely against lipopolysaccharide (LPS). After boosting with sonicated organisms, the specificity repertoire broadened against protein antigens, including DnaK, LpnA, FopA, bacterioferritin, the 50S ribosomal protein L7\/L12, and metabolic enzymes. These monoclonal antibodies detect<jats:italic>F. tularensis<\/jats:italic>LVS by routine immunoassays, including enzyme-linked immunosorbent assay, Western blot analysis, and immunofluorescence. The ability of the antibodies to protect mice from intradermal infection, both prophylactically and therapeutically, was examined. An antibody to LPS which provides complete protection from infection with<jats:italic>F. tularensis<\/jats:italic>LVS and partial protection from infection with<jats:italic>F. tularensis<\/jats:italic>subsp.<jats:italic>tularensis<\/jats:italic>strain SchuS4 was identified. There was no bacteremia and reduced organ burden within the first 24 h when mice were protected from<jats:italic>F. tularensis<\/jats:italic>LVS infection with the anti-LPS antibody. No antibody that provided complete protection when administered therapeutically was identified; however, passive transfer of antibodies against LPS, FopA, and LpnA resulted in 40 to 50% survival of mice infected with<jats:italic>F. tularensis<\/jats:italic>LVS.<\/jats:p>","DOI":"10.1128\/cvi.00362-08","type":"journal-article","created":{"date-parts":[[2009,1,29]],"date-time":"2009-01-29T01:58:59Z","timestamp":1233194339000},"page":"414-422","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":51,"title":["<i>Francisella tularensis<\/i>Infection-Derived Monoclonal Antibodies Provide Detection, Protection, and Therapy"],"prefix":"10.1128","volume":"16","author":[{"given":"Anne G.","family":"Savitt","sequence":"first","affiliation":[{"name":"Center for Infectious Disease, Department of Molecular Genetics and Microbiology, Stony Brook University, Stony Brook, New York 11794"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patricio","family":"Mena-Taboada","sequence":"additional","affiliation":[{"name":"Center for Infectious Disease, Department of Molecular Genetics and Microbiology, Stony Brook University, Stony Brook, New York 11794"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gloria","family":"Monsalve","sequence":"additional","affiliation":[{"name":"Center for Infectious Disease, Department of Molecular Genetics and Microbiology, Stony Brook University, Stony Brook, New York 11794"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jorge L.","family":"Benach","sequence":"additional","affiliation":[{"name":"Center for Infectious Disease, Department of Molecular Genetics and Microbiology, Stony Brook University, Stony Brook, New York 11794"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1084\/jem.115.2.411","volume":"115","year":"1962","unstructured":"Allen, W. P. 1962. Immunity against tularemia: passive protection of mice by transfer of immune tissues. J. Exp. Med.115:411-420.","journal-title":"J. Exp. Med."},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.4.1915-1923.2002"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.10.6264-6270.2001"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.02011-05"},{"key":"e_1_3_2_6_2","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1016\/j.tvjl.2004.04.021","volume":"170","year":"2005","unstructured":"Berry, J. D. 2005. Rational monoclonal antibody development to emerging pathogens, biothreat agents and agents of foreign animal disease: the antigen scale. Vet. J.170:193-211.","journal-title":"Vet. J."},{"key":"e_1_3_2_7_2","doi-asserted-by":"crossref","first-page":"833","DOI":"10.3201\/eid0808.010516","volume":"8","year":"2002","unstructured":"Casadevall, A. 2002. Passive antibody adminstration (immediate immunity) as a specific defense against biological weapons. Emerg. Infect. Dis.8:833-841.","journal-title":"Emerg. Infect. Dis."},{"key":"e_1_3_2_8_2","doi-asserted-by":"crossref","first-page":"695","DOI":"10.1038\/nrmicro974","volume":"2","year":"2004","unstructured":"Casadevall, A., E. Dadachova, and L.-A. Pirofski. 2004. Passive antibody therapy for infectious diseases. Nat. Rev. Microbiol.2:695-703.","journal-title":"Nat. Rev. Microbiol."},{"key":"e_1_3_2_9_2","first-page":"181","volume":"51","year":"2002","unstructured":"CDC. 2002. Tularemia\u2014United States, 1990-2000. MMWR Morb. Mortal. Wkly. Rep.51:181-184.","journal-title":"MMWR Morb. Mortal. Wkly. Rep."},{"key":"e_1_3_2_10_2","unstructured":"Reference deleted."},{"key":"e_1_3_2_11_2","doi-asserted-by":"crossref","first-page":"2763","DOI":"10.1001\/jama.285.21.2763","volume":"285","year":"2001","unstructured":"Dennis, D. T., T. V. Inglesby, D. A. Henderson, J. G. Bartlett, M. S. Ascher, E. Eitzen, A. D. Fine, A. M. Friedlander, J. Hauer, M. Layton, S. R. Lillibridge, J. E. McDade, M. T. Osterholm, T. O'Toole, G. Parker, T. M. Perl, P. K. Russell, K. Tonat, et al. 2001. Tularemia as a biological weapon: medical and public health management. JAMA285:2763-2773.","journal-title":"JAMA"},{"key":"e_1_3_2_12_2","first-page":"785","volume":"18","year":"2005","unstructured":"Due\u00f1as, A. I., M. Aceves, A. Ordu\u00f1a, R. D\u00edaz, M. S. Crespo, and C. Garc\u00eda-Rodr\u00edguez. 2005. Francisella tularensis LPS induces the production of cytokines in human monocytes and signals via Toll-like receptor 4 with much lower potency than E. coli LPS. Int. Immunol.18:785-795.","journal-title":"Int. Immunol."},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.15.4.631-646.2002"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.62.1.178-183.1994"},{"key":"e_1_3_2_15_2","doi-asserted-by":"crossref","first-page":"2172","DOI":"10.1002\/pmic.200600985","volume":"7","year":"2007","unstructured":"Eyles, J. E., B. Unal, M. G. Hartley, S. L. Newstead, H. Flick-Smith, J. L. Prior, P. C. F. Oyston, A. Randall, Y. Mu, S. Hirst, D. M. Molina, D. H. Davies, T. Milne, K. F. Griffin, P. Baldi, R. W. Titball, and P. L. Felgner. 2007. Immunodominant Francisella tularensis antigens identified using proteome microarray. Proteomics7:2172-2183.","journal-title":"Proteomics"},{"key":"e_1_3_2_16_2","first-page":"1835","volume":"51","year":"2005","unstructured":"Farlow, J., D. M. Wagner, M. Dukerich, M. Stanley, M. C. Chu, K. Kubota, J. M. Petersen, and P. Keim. 2005. Francisella tularensis in the United States. Emerg. Infect. Dis.51:1835-1841.","journal-title":"Emerg. Infect. Dis."},{"key":"e_1_3_2_17_2","doi-asserted-by":"crossref","first-page":"2563","DOI":"10.4049\/jimmunol.171.5.2563","volume":"171","year":"2003","unstructured":"Forestal, C. A., J. L. Benach, C. Carbonara, J. K. Italo, T. J. Lisinski, and M. B. Furie. 2003. Francisella tularensis selectively induces proinflammatory changes in endothelial cells. J. Immunol.171:2563-2570.","journal-title":"J. Immunol."},{"key":"e_1_3_2_18_2","doi-asserted-by":"crossref","first-page":"512","DOI":"10.1016\/j.micpath.2008.01.003","volume":"44","year":"2008","unstructured":"Forestal, C. A., H. Gil, M. Monfett, C. E. Noah, G. J. Platz, D. G. Thanassi, J. L. Benach, and M. B. Furie. 2008. A conserved and immunodominant lipoprotein of Francisella tularensis is proinflammatory but not essential for virulence. Microb. Pathog.44:512-523.","journal-title":"Microb. Pathog."},{"key":"e_1_3_2_19_2","doi-asserted-by":"crossref","first-page":"180","DOI":"10.1016\/0002-9343(46)90036-8","volume":"1","year":"1946","unstructured":"Foshay, L. 1946. A comparative study of the treatment of tularemia with immune serum, hyperimmune serum and streptomycin. Am. J. Med.1:180-188.","journal-title":"Am. J. Med."},{"key":"e_1_3_2_20_2","doi-asserted-by":"crossref","first-page":"1220","DOI":"10.1016\/0264-410X(95)00062-6","volume":"13","year":"1995","unstructured":"Fulop, M., R. Manchee, and R. Titball. 1995. Role of lipopolysaccharide and a major outer membrane protein from Francisella tularensis in the induction of immunity against tularemia. Vaccine13:1220-1225.","journal-title":"Vaccine"},{"key":"e_1_3_2_21_2","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1111\/j.1574-695X.1996.tb00245.x","volume":"13","year":"1996","unstructured":"Fulop, M., R. Manchee, and R. Titball. 1996. Role of two outer membrane antigens in the induction of protective immunity against Francisella tularensis strains of different virulence. FEMS Immunol. Med. Microbiol.13:245-247.","journal-title":"FEMS Immunol. Med. Microbiol."},{"key":"e_1_3_2_22_2","doi-asserted-by":"crossref","first-page":"4465","DOI":"10.1016\/S0264-410X(01)00189-X","volume":"19","year":"2001","unstructured":"Fulop, M., P. Mastroeni, M. Green, and R. W. Titball. 2001. Role of antibody to lipopolysaccharide in protection against low- and high-virulence strains of Francisella tularensis. Vaccine19:4465-4472.","journal-title":"Vaccine"},{"key":"e_1_3_2_23_2","doi-asserted-by":"crossref","first-page":"1699","DOI":"10.1534\/genetics.104.68262","volume":"174","year":"2006","unstructured":"Georgopoulos, C. 2006. Toothpicks, serendipity and the emergence of the Escherichia coli DnaK (Hsp70) and GroEL (Hsp60) chaperone machines. Genetics174:1699-1707.","journal-title":"Genetics"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.8.4818-4822.2003"},{"key":"e_1_3_2_25_2","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1016\/0264-410X(95)93311-V","volume":"13","year":"1995","unstructured":"Golovliov, I., M. Ericsson, L. Akerblom, G. Sandstrom, A. Tarnvik, and A. Sjostedt. 1995. Adjuvanticity of ISCOMs incorporating a T cell-reactive lipoprotein of the facultative intracellular pathogen Francisella tularensis. Vaccine13:261.","journal-title":"Vaccine"},{"key":"e_1_3_2_26_2","doi-asserted-by":"crossref","first-page":"2680","DOI":"10.1016\/j.vaccine.2004.03.071","volume":"23","year":"2005","unstructured":"Green, M., G. Choules, D. Rogers, and R. W. Titball. 2005. Efficacy of the live attenuated Francisella tularensis vaccine (LVS) in a murine model of disease. Vaccine23:2680-2686.","journal-title":"Vaccine"},{"key":"e_1_3_2_27_2","doi-asserted-by":"crossref","first-page":"1159","DOI":"10.1016\/S1567-5769(03)00028-6","volume":"3","year":"2003","unstructured":"Hengel, H., and K. N. Masihi. 2003. Combinatorial immunotherapies for infectious diseases. Int. Immunopharmacol.3:1159-1167.","journal-title":"Int. Immunopharmacol."},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01505-06"},{"key":"e_1_3_2_29_2","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1016\/j.imlet.2006.12.004","volume":"108","year":"2007","unstructured":"Janovska, S., I. Pavkova, M. Hubalek, J. Lenco, A. Macela, and J. Stulik. 2007. Identification of immunoreactive antigens in membrane proteins enriched fraction from Francisella tularensis LVS. Immunol. Lett.108:151-159.","journal-title":"Immunol. Lett."},{"key":"e_1_3_2_30_2","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1016\/j.femsim.2004.12.001","volume":"44","year":"2005","unstructured":"Jomaa, M., J. M. Kyd, and A. W. Cripps. 2005. Mucosal immunisation with novel Streptococcus pneumoniae protein antigens enhances bacterial clearance in an acute mouse lung infection model. FEMS Immunol. Med. Microbiol.44:59-67.","journal-title":"FEMS Immunol. Med. Microbiol."},{"key":"e_1_3_2_31_2","doi-asserted-by":"crossref","first-page":"532","DOI":"10.4049\/jimmunol.179.1.532","volume":"179","year":"2007","unstructured":"Kirimanjeswara, G. S., J. M. Golden, C. S. Bakshi, and D. W. Metzger. 2007. Prophylactic and therapeutic use of antibodies for protection against respiratory infection with Francisella tularensis. J. Immunol.179:532-539.","journal-title":"J. Immunol."},{"key":"e_1_3_2_32_2","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1038\/ng1499","volume":"37","year":"2005","unstructured":"Larsson, P., P. C. Oyston, P. Chain, M. C. Chu, M. Duffield, H. H. Fuxelius, E. Garcia, G. Halltorp, D. Johansson, K. E. Isherwood, P. D. Karp, E. Larsson, Y. Liu, S. Michell, J. Prior, R. Prior, S. Malfatti, A. Sjostedt, K. Svensson, N. Thompson, L. Vergez, J. K. Wagg, B. W. Wren, L. E. Lindler, S. G. Andersson, M. Forsman, and R. W. Titball. 2005. The complete genome sequence of Francisella tularensis, the causative agent of tularemia. Nat. Genet.37:153-159.","journal-title":"Nat. Genet."},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00257-06"},{"key":"e_1_3_2_34_2","doi-asserted-by":"crossref","first-page":"1221","DOI":"10.1099\/jmm.0.45616-0","volume":"53","year":"2004","unstructured":"Litwin, C. M., J. M. Johnson, and T. B. Martins. 2004. The Bartonella henselae sucB gene encodes a dihydrolipoamide succinyltransferase protein reactive with sera from patients with cat-scratch disease. J. Med. Microbiol.53:1221-1227.","journal-title":"J. Med. Microbiol."},{"key":"e_1_3_2_35_2","first-page":"331","volume":"119","year":"2006","unstructured":"Luo, D., P. Li, L. Xing, G. Zhao, W. Shi, S. Zhang, and X. Wang. 2006. DNA vaccine encoding L7\/L12-P39 of Brucella abortus induces protective immunity in BALB\/c mice. Chin. Med. J. (Engl.)119:331-334.","journal-title":"Chin. Med. J. (Engl.)"},{"key":"e_1_3_2_36_2","doi-asserted-by":"crossref","first-page":"3692","DOI":"10.1016\/j.vaccine.2007.01.066","volume":"25","year":"2007","unstructured":"Mallick, A., H. Singha, P. Chaudhuri, A. Nadeem, S. A. Khan, K. A. Dar, and M. Owais. 2007. Liposomised recombinant ribosomal L7\/L12 protein protects BALB\/c mice against Brucella abortus 544 infection. Vaccine25:3692-3704.","journal-title":"Vaccine"},{"key":"e_1_3_2_37_2","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1146\/annurev.micro.60.080805.142126","volume":"60","year":"2006","unstructured":"McLendon, M. K., M. A. Apicella, and L.-A. H. Allen. 2006. Francisella tularensis: taxonomy, genetics and immunopathogenesis of a potential agent of biowarfare. Annu. Rev. Microbiol.60:167-185.","journal-title":"Annu. Rev. Microbiol."},{"key":"e_1_3_2_38_2","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1111\/j.1574-6968.2006.00413.x","volume":"263","year":"2006","unstructured":"Melillo, A., D. D. Sledjeski, S. Lipski, R. M. Wooten, V. Basrur, and E. R. Lafontaine. 2006. Identification of a Francisella tularensis LVS outer membrane protein that confers adherence to A549 human lung cells. FEMS Microbiol. Lett.263:102-108.","journal-title":"FEMS Microbiol. Lett."},{"key":"e_1_3_2_39_2","doi-asserted-by":"crossref","first-page":"743","DOI":"10.1111\/j.1348-0421.1999.tb02465.x","volume":"43","year":"1999","unstructured":"Nguyen, S., H. To, T. Yamaguchi, H. Fukushi, and K. Hirai. 1999. Characterization of the Coxiella burnetti sucB gene encoding an immunogenic dihydrolipoamide succinyltransferase. Microbiol. Immunol.43:743-749.","journal-title":"Microbiol. Immunol."},{"key":"e_1_3_2_40_2","doi-asserted-by":"crossref","first-page":"967","DOI":"10.1038\/nrmicro1045","volume":"2","year":"2004","unstructured":"Oyston, P. C., A. Sjostedt, and R. W. Titball. 2004. Tularaemia: bioterrorism defence renews interest in Francisella tularensis. Nat. Rev. Microbiol.2:967-978.","journal-title":"Nat. Rev. Microbiol."},{"key":"e_1_3_2_41_2","doi-asserted-by":"crossref","first-page":"3125","DOI":"10.1021\/pr0601887","volume":"5","year":"2006","unstructured":"Pavkova, I., M. Reichelova, P. Larsson, M. Hubalek, J. Vackova, A. Forsberg, and J. Stulik. 2006. Comparative proteome analysis of fractions enriched for membrane-associated proteins from Francisella tularensis subsp. tularensis and F. tularensis subsp. holarctica strains. J. Proteome Res.5:3125-3134.","journal-title":"J. Proteome Res."},{"key":"e_1_3_2_42_2","doi-asserted-by":"crossref","first-page":"506","DOI":"10.1128\/CDLI.10.4.506-513.2003","volume":"10","year":"2003","unstructured":"Peruski, A. H., and L. F. Peruski. 2003. Immunological methods for detection and identification of infectious disease and biological warfare agents. Clin. Diagn. Lab. Immunol.10:506-513.","journal-title":"Clin. Diagn. Lab. Immunol."},{"key":"e_1_3_2_43_2","doi-asserted-by":"crossref","first-page":"813","DOI":"10.1189\/jlb.1206765","volume":"82","year":"2007","unstructured":"Rahhal, R. M., T. J. Vanden Bush, M. K. McLendon, M. A. Apicella, and G. A. Bishop. 2007. Differential effects of Francisella tularensis lipopolysaccharide on B lymphocytes. J. Leukoc. Biol.82:813-820.","journal-title":"J. Leukoc. Biol."},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00868-06"},{"key":"e_1_3_2_45_2","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1089\/hyb.1986.5.255","volume":"5","year":"1986","unstructured":"Rathjen, D. A., and C. L. Geczy. 1986. Conditioned medium from macrophage cell lines supports the single-cell growth of hybridomas. Hybridoma5:255-261.","journal-title":"Hybridoma"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.62.8.3129-3137.1994"},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01006-06"},{"key":"e_1_3_2_48_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid0802.010164"},{"key":"e_1_3_2_49_2","first-page":"53","volume":"25","year":"2007","unstructured":"Sakolvaree, Y., S. Maneewatch, S. Jiemsup, B. Klaysing, P. Tongtawe, P. Srimanote, P. Saengjaruk, S. Banyen, P. Tapchaisri, M. Chonsa-nquan, and W. Chaicumpa. 2007. Proteome and immunome of pathogenic Leptospira spp. revealed by 2DE and 2DE-immunoblotting with immune serum. Asian Pac. J. Allergy Immunol.25:53-73.","journal-title":"Asian Pac. J. Allergy Immunol."},{"key":"e_1_3_2_50_2","unstructured":"Molecular cloning: a laboratory manual 1989"},{"key":"e_1_3_2_51_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.60.7.2855-2862.1992"},{"key":"e_1_3_2_52_2","doi-asserted-by":"crossref","first-page":"311","DOI":"10.4049\/jimmunol.145.1.311","volume":"145","year":"1990","unstructured":"Sjostedt, A., G. Sandstrom, A. Tarnvik, and B. Jaurin. 1990. Nucleotide sequence and T cell epitopes of a membrane protein of Francisella tularensis. J. Immunol.145:311-317.","journal-title":"J. Immunol."},{"key":"e_1_3_2_53_2","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1016\/S0882-4010(03)00095-0","volume":"35","year":"2003","unstructured":"Stenmark, S., H. Lindgren, A. Tarnvik, and A. Sjostedt. 2003. Specific antibodies contribute to the host protection against strains of Francisella tularensis subspecies holarctica. Microb. Pathog.35:73-80.","journal-title":"Microb. Pathog."},{"key":"e_1_3_2_54_2","doi-asserted-by":"crossref","first-page":"501","DOI":"10.1099\/jmm.0.05352-0","volume":"53","year":"2004","unstructured":"Stenmark, S., and A. Sjostedt. 2004. Transfer of specific antibodies results in increased expression of TNF-\u03b1 and IL12 and recruitment of neutrophils to the site of a cutaneous Francisella tularensis infection. J. Med. Microbiol.53:501-504.","journal-title":"J. Med. Microbiol."},{"key":"e_1_3_2_55_2","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1016\/0022-1759(85)90106-1","volume":"79","year":"1985","unstructured":"Sugasawara, R. J., B. E. Cahoon, and A. E. Karu. 1985. The influence of murine macrophage-conditioned medium on cloning efficiency, antibody synthesis and growth rate of hybridomas. J. Immunol. Methods79:263-275.","journal-title":"J. Immunol. Methods"},{"key":"e_1_3_2_56_2","doi-asserted-by":"crossref","first-page":"1491","DOI":"10.1002\/elps.1150180824","volume":"18","year":"1997","unstructured":"Teixeira-Gomes, A., A. Cloeckaert, G. Bezard, R. Bowden, G. Dubray, and M. Zygmunt. 1997. Identification and characterization of Brucella ovis immunogenic proteins using two-dimensional electrophoresis and immunoblotting. Electrophoresis18:1491-1497.","journal-title":"Electrophoresis"},{"key":"e_1_3_2_57_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.76.9.4350"},{"key":"e_1_3_2_58_2","doi-asserted-by":"crossref","first-page":"999","DOI":"10.1016\/j.bbrc.2006.06.008","volume":"346","year":"2006","unstructured":"Twine, S. M., M. D. Petit, H. Shen, N. C. S. Mykytczuk, J. F. Kelly, and J. W. Conlan. 2006. Immunoproteomic analysis of the murine antibody response to successful and failed immunization with live anti-Francisella vaccines. Biochem. Biophys. Res. Commun.346:999-1008.","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"e_1_3_2_59_2","doi-asserted-by":"crossref","first-page":"6112","DOI":"10.1046\/j.1432-1033.2002.03321.x","volume":"269","year":"2002","unstructured":"Vinogradov, E., M. B. Perry, and J. W. Conlan. 2002. Structural analysis of Francisella tularensis lipopolysaccharide. Eur. J. Biochem.269:6112-6118.","journal-title":"Eur. J. Biochem."},{"key":"e_1_3_2_60_2","first-page":"739","volume":"386","year":"2005","unstructured":"Weibezahn, J., C. Schlieker, T. Peter, A. Mogk, and B. Bukau. 2005. Novel insights into the mechanism of chaperone-assisted protein disaggregation. Biol. Chem.386:739-744.","journal-title":"Biol. Chem."},{"key":"e_1_3_2_61_2","first-page":"E060928.3","volume":"11","year":"2006","unstructured":"Wik, O. 2006. Large tularaemia outbreak in Varmland, central Sweden, 2006. Euro Surveill.11:E060928.3.","journal-title":"Euro Surveill."},{"key":"e_1_3_2_62_2","doi-asserted-by":"crossref","first-page":"3692","DOI":"10.1016\/j.vaccine.2007.01.066","volume":"25","year":"2007","unstructured":"Yu, D.-H., X.-D. Hu, H. Cai, and M. Li. 2007. A combined DNA vaccine encoding BCSP31, SOD and L7\/L12 confers high protection against Brucella abortus 2308 by inducing specific CTL responses. Vaccine25:3692-3704.","journal-title":"Vaccine"},{"key":"e_1_3_2_63_2","first-page":"149","volume":"164","year":"1995","unstructured":"Zuber, M., T. A. Hoover, M. T. Dertzbaugh, and D. L. Court. 1995. Analysis of the DnaK molecular chaperone system of Francisella tularensis. Genetics164:149-152.","journal-title":"Genetics"},{"key":"e_1_3_2_64_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.10.6537-6540.2001"}],"container-title":["Clinical and Vaccine Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/CVI.00362-08","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/CVI.00362-08","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,5,23]],"date-time":"2023-05-23T20:02:52Z","timestamp":1684872172000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/CVI.00362-08"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,3]]},"references-count":63,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2009,3]]}},"alternative-id":["10.1128\/CVI.00362-08"],"URL":"https:\/\/doi.org\/10.1128\/cvi.00362-08","relation":{},"ISSN":["1556-6811","1556-679X"],"issn-type":[{"value":"1556-6811","type":"print"},{"value":"1556-679X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,3]]},"assertion":[{"value":"2008-10-02","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2009-01-06","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-12-18","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}