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The vaccinated dogs produced high titers of anti-OspA and anti-OspC borreliacidal antibodies, including borreliacidal antibodies specific for an epitope within the last seven amino acids at the OspC carboxy terminus (termed OspC7) that was conserved among pathogenic<jats:italic>Borrelia<\/jats:italic>genospecies. In addition, spirochetes were eliminated from the infected ticks that fed on the bacterin recipients,<jats:italic>B. burgdorferi<\/jats:italic>was not isolated from the skin or joints, and antibody responses associated specifically with canine infection with<jats:italic>B. burgdorferi<\/jats:italic>were not produced. In contrast,<jats:italic>B. burgdorferi<\/jats:italic>was recovered from engorged ticks that fed on 13 (87%) placebo-vaccinated dogs (<jats:italic>P<\/jats:italic>&lt; 0.0001), skin biopsy specimens from 14 (93%) dogs (<jats:italic>P<\/jats:italic>&lt; 0.0001), and joint tissue specimens from 8 (53%) dogs (<jats:italic>P<\/jats:italic>= 0.0022). In addition, 14 (93%) dogs developed specific antibody responses against<jats:italic>B. burgdorferi<\/jats:italic>proteins, including 11 (73%) with C6 peptide antibodies (<jats:italic>P<\/jats:italic>&lt; 0.0001). Moreover, 10 (67%) dogs developed Lyme disease-associated joint abnormalities (<jats:italic>P<\/jats:italic>&lt; 0.0001), including 4 (27%) dogs that developed joint stiffness or lameness and 6 (40%) that developed chronic joint inflammation (synovitis). The results therefore confirmed that the bacterin provided a high level of protection against Lyme disease shortly after immunization.<\/jats:p>","DOI":"10.1128\/cvi.00373-08","type":"journal-article","created":{"date-parts":[[2008,12,4]],"date-time":"2008-12-04T02:30:49Z","timestamp":1228357849000},"page":"253-259","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":45,"title":["Bacterin That Induces Anti-OspA and Anti-OspC Borreliacidal Antibodies Provides a High Level of Protection against Canine Lyme Disease"],"prefix":"10.1128","volume":"16","author":[{"given":"Rhonda L.","family":"LaFleur","sequence":"first","affiliation":[{"name":"Schering Plough Animal Health Corporation, Elkhorn, Nebraska 68022"},{"name":"Gundersen Lutheran Medical Center, La Crosse, Wisconsin 54601"},{"name":"University of Wisconsin, Madison, Wisconsin 53706"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer C.","family":"Dant","sequence":"additional","affiliation":[{"name":"Schering Plough Animal Health Corporation, Elkhorn, Nebraska 68022"},{"name":"Gundersen Lutheran Medical Center, La Crosse, Wisconsin 54601"},{"name":"University of Wisconsin, Madison, Wisconsin 53706"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Terri L.","family":"Wasmoen","sequence":"additional","affiliation":[{"name":"Schering Plough Animal Health Corporation, Elkhorn, Nebraska 68022"},{"name":"Gundersen Lutheran Medical Center, La Crosse, Wisconsin 54601"},{"name":"University of Wisconsin, Madison, Wisconsin 53706"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven M.","family":"Callister","sequence":"additional","affiliation":[{"name":"Schering Plough Animal Health Corporation, Elkhorn, Nebraska 68022"},{"name":"Gundersen Lutheran Medical Center, La Crosse, Wisconsin 54601"},{"name":"University of Wisconsin, Madison, Wisconsin 53706"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dean A.","family":"Jobe","sequence":"additional","affiliation":[{"name":"Schering Plough Animal Health Corporation, Elkhorn, Nebraska 68022"},{"name":"Gundersen Lutheran Medical Center, La Crosse, Wisconsin 54601"},{"name":"University of Wisconsin, Madison, Wisconsin 53706"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven D.","family":"Lovrich","sequence":"additional","affiliation":[{"name":"Schering Plough Animal Health Corporation, Elkhorn, Nebraska 68022"},{"name":"Gundersen Lutheran Medical Center, La Crosse, Wisconsin 54601"},{"name":"University of Wisconsin, Madison, Wisconsin 53706"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas F.","family":"Warner","sequence":"additional","affiliation":[{"name":"Schering Plough Animal Health Corporation, Elkhorn, Nebraska 68022"},{"name":"Gundersen Lutheran Medical Center, La Crosse, Wisconsin 54601"},{"name":"University of Wisconsin, Madison, Wisconsin 53706"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"O.","family":"Abdelmagid","sequence":"additional","affiliation":[{"name":"Schering Plough Animal Health Corporation, Elkhorn, Nebraska 68022"},{"name":"Gundersen Lutheran Medical Center, La Crosse, Wisconsin 54601"},{"name":"University of Wisconsin, Madison, Wisconsin 53706"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ronald F.","family":"Schell","sequence":"additional","affiliation":[{"name":"Schering Plough Animal Health Corporation, Elkhorn, Nebraska 68022"},{"name":"Gundersen Lutheran Medical Center, La Crosse, Wisconsin 54601"},{"name":"University of Wisconsin, Madison, Wisconsin 53706"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_1_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.34.3.524-529.1996"},{"key":"e_1_3_1_3_2","doi-asserted-by":"crossref","first-page":"651","DOI":"10.1093\/infdis\/167.3.651","volume":"167","year":"1993","unstructured":"Appel, M. 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