{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,16]],"date-time":"2026-05-16T03:07:34Z","timestamp":1778900854519,"version":"3.51.4"},"reference-count":30,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2014,10,1]],"date-time":"2014-10-01T00:00:00Z","timestamp":1412121600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Vaccine Immunol"],"published-print":{"date-parts":[[2014,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The laboratory diagnosis of Lyme disease is based upon the detection of antibodies generated against\n            <jats:named-content content-type=\"genus-species\">Borrelia burgdorferi<\/jats:named-content>\n            using a two-tier assay, typically consisting of an enzyme-linked immunosorbent assay (ELISA), followed by a Western blot. This system, put into place to address the nonspecificity associated with standalone first-tier assays, is insensitive for diagnosing early infection, when most people seek care. The use of bacterial lysates or whole-protein antigens as first-tier assay targets contributes to nonspecificity due, in part, to the presence of cross-reactive epitopes that are also found in other bacteria. This precludes their use as sensitive standalone assays. The use of peptides containing linear epitopes that are highly specific for\n            <jats:named-content content-type=\"genus-species\">B. burgdorferi<\/jats:named-content>\n            offers a method for reducing this cross-reactivity. In the present study, we mapped the linear epitopes of the prominently expressed\n            <jats:named-content content-type=\"genus-species\">Borrelia<\/jats:named-content>\n            adhesins decorin binding protein A (DbpA) and DbpB. We identified several epitopes in each protein that were highly conserved among North American strains of\n            <jats:named-content content-type=\"genus-species\">B. burgdorferi<\/jats:named-content>\n            , and we screened peptides containing specific epitopes using serum panels from early and late Lyme disease patients. The individual peptides primarily detected IgM but not IgG, while the proteins efficiently detected both IgM and IgG. While no individual peptide demonstrated better utility for antibody detection than its respective whole protein, an assay containing a combination of a DbpA and a DbpB peptide adequately detected both IgM and IgG, accurately identifying 87.5% (84\/96) of the early Lyme disease patients and 80.0% (16\/20) of the late Lyme disease patients.\n          <\/jats:p>","DOI":"10.1128\/cvi.00383-14","type":"journal-article","created":{"date-parts":[[2014,8,14]],"date-time":"2014-08-14T04:34:48Z","timestamp":1407990888000},"page":"1426-1436","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":23,"title":["Decorin Binding Proteins A and B in the Serodiagnosis of Lyme Disease in North America"],"prefix":"10.1128","volume":"21","author":[{"given":"Paul M.","family":"Arnaboldi","sequence":"first","affiliation":[{"name":"Biopeptides Corp., East Setauket, New York, USA"},{"name":"Department of Microbiology and Immunology, New York Medical College, Valhalla, New York, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mariya","family":"Sambir","sequence":"additional","affiliation":[{"name":"Biopeptides Corp., East Setauket, New York, USA"},{"name":"Department of Microbiology and Immunology, New York Medical College, Valhalla, New York, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Raymond J.","family":"Dattwyler","sequence":"additional","affiliation":[{"name":"Biopeptides Corp., East Setauket, New York, USA"},{"name":"Department of Microbiology and Immunology, New York Medical College, Valhalla, New York, USA"},{"name":"Department of Medicine, New York Medical College, Valhalla, New York, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(11)60103-7"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM200107123450207"},{"key":"e_1_3_3_4_2","doi-asserted-by":"crossref","first-page":"502","DOI":"10.1016\/S0002-9343(95)99915-9","article-title":"The clinical spectrum of early Lyme borreliosis in patients with culture-confirmed erythema migrans","volume":"100","author":"Nadelman RB","year":"1996","unstructured":"NadelmanRBNowakowskiJForseterGGoldbergNSBittkerSCooperDAguero-RosenfeldMWormserGP. 1996. The clinical spectrum of early Lyme borreliosis in patients with culture-confirmed erythema migrans. Am. J. Med. 100:502\u2013508.","journal-title":"Am. J. Med."},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid1905.120796"},{"key":"e_1_3_3_6_2","first-page":"590","article-title":"Recommendations for test performance and interpretation from the Second National Conference on Serologic Diagnosis of Lyme Dis","volume":"44","author":"Centers for Disease Control and Prevention (CDC)","year":"1995","unstructured":"Centers for Disease Control and Prevention (CDC). 1995. Recommendations for test performance and interpretation from the Second National Conference on Serologic Diagnosis of Lyme Dis. MMWR Morb. Mortal. Wkly. Rep. 44:590\u2013591.","journal-title":"MMWR Morb. Mortal. Wkly. 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Molecular analysis of sequence heterogeneity among genes encoding decorin binding proteins A and B of Borrelia burgdorferi sensu lato. Infect. Immun. 66:5275\u20135285.","journal-title":"Infect. 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