{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,2]],"date-time":"2025-10-02T10:46:13Z","timestamp":1759401973803},"reference-count":30,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2007,7,1]],"date-time":"2007-07-01T00:00:00Z","timestamp":1183248000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Vaccine Immunol"],"published-print":{"date-parts":[[2007,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            A successful vaccine vector for human immunodeficiency virus type 1 (HIV-1) should induce anti-HIV-1 T-cell immune responses at mucosal sites. We have constructed recombinant\n            <jats:italic>Mycobacterium bovis<\/jats:italic>\n            bacillus Calmette-Gue\u0301rin (rBCG) expressing an HIV-1 group M consensus envelope (Env) either as a surface, intracellular, or secreted protein as an immunogen. rBCG containing HIV-1\n            <jats:italic>env<\/jats:italic>\n            plasmids engineered for secretion induced optimal Env-specific T-cell gamma interferon enzyme-linked immunospot responses in murine spleen, female reproductive tract, and lungs. While rBCG-induced T-cell responses to HIV-1 envelope in spleen were lower than those induced by adenovirus prime\/recombinant vaccinia virus (rAd-rVV) boost, rBCG induced comparable responses to rAd-rVV immunization in the female reproductive tract and lungs. T-cell responses induced by rBCG were primarily CD4\n            <jats:sup>+<\/jats:sup>\n            , although rBCG alone did not induce anti-HIV-1 antibody. However, rBCG could prime for a protein boost by HIV-1 envelope protein. Thus, rBCG can serve as a vector for induction of anti-HIV-1 consensus Env cellular responses at mucosal sites.\n          <\/jats:p>","DOI":"10.1128\/cvi.00407-06","type":"journal-article","created":{"date-parts":[[2007,5,17]],"date-time":"2007-05-17T00:54:46Z","timestamp":1179363286000},"page":"886-893","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":20,"title":["Recombinant\n            <i>Mycobacterium bovis<\/i>\n            Bacillus Calmette-Gue\u0301rin Elicits Human Immunodeficiency Virus Type 1 Envelope-Specific T Lymphocytes at Mucosal Sites"],"prefix":"10.1128","volume":"14","author":[{"given":"Jae-Sung","family":"Yu","sequence":"first","affiliation":[{"name":"Human Vaccine Institute and Department of Medicine, Duke University Medical Center, Durham, North Carolina 27710"},{"name":"Howard Hughes Medical Institute and Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, New York 10461"},{"name":"Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts 02215"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James W.","family":"Peacock","sequence":"additional","affiliation":[{"name":"Human Vaccine Institute and Department of Medicine, Duke University Medical Center, Durham, North Carolina 27710"},{"name":"Howard Hughes Medical Institute and Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, New York 10461"},{"name":"Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts 02215"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"Jr.","given":"William R.","family":"Jacobs","sequence":"additional","affiliation":[{"name":"Human Vaccine Institute and Department of Medicine, Duke University Medical Center, Durham, North Carolina 27710"},{"name":"Howard Hughes Medical Institute and Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, New York 10461"},{"name":"Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts 02215"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard","family":"Frothingham","sequence":"additional","affiliation":[{"name":"Human Vaccine Institute and Department of Medicine, Duke University Medical Center, Durham, North Carolina 27710"},{"name":"Howard Hughes Medical Institute and Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, New York 10461"},{"name":"Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts 02215"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Norman L.","family":"Letvin","sequence":"additional","affiliation":[{"name":"Human Vaccine Institute and Department of Medicine, Duke University Medical Center, Durham, North Carolina 27710"},{"name":"Howard Hughes Medical Institute and Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, New York 10461"},{"name":"Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts 02215"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hua-Xin","family":"Liao","sequence":"additional","affiliation":[{"name":"Human Vaccine Institute and Department of Medicine, Duke University Medical Center, Durham, North Carolina 27710"},{"name":"Howard Hughes Medical Institute and Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, New York 10461"},{"name":"Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts 02215"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Barton F.","family":"Haynes","sequence":"additional","affiliation":[{"name":"Human Vaccine Institute and Department of Medicine, Duke University Medical Center, Durham, North Carolina 27710"},{"name":"Howard Hughes Medical Institute and Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, New York 10461"},{"name":"Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts 02215"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.2.672-678.2002"},{"key":"e_1_3_2_3_2","unstructured":"Expression of foreign genes in mycobacteria. 1994"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.80.4.1645-1652.2006"},{"key":"e_1_3_2_5_2","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1016\/j.tube.2004.09.007","volume":"85","year":"2005","unstructured":"de Lisle, G. W., B. J. Wards, B. M. Buddle, and D. M. Collins. 2005. The efficacy of live tuberculosis vaccines after presensitization with Mycobacterium avium. Tuberculosis (Edinburgh)85:73-79.","journal-title":"Tuberculosis (Edinburgh)"},{"key":"e_1_3_2_6_2","doi-asserted-by":"crossref","first-page":"718","DOI":"10.1126\/science.276.5313.718","volume":"276","year":"1997","unstructured":"Finlay, B. B., and P. Cossart. 1997. Exploitation of mammalian host cell functions by bacterial pathogens. Science276:718-725.","journal-title":"Science"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.79.2.1154-1163.2005"},{"key":"e_1_3_2_8_2","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1038\/nm1380","volume":"12","year":"2006","unstructured":"Grossman, Z., M. Meier-Schellersheim, W. E. Paul, and L. J. Picker. 2006. Pathogenesis of HIV infection: what the virus spares is as important as what it destroys. Nat. Med.12:289-295.","journal-title":"Nat. Med."},{"key":"e_1_3_2_9_2","unstructured":"Molecular genetics of mycobacteria. 2000"},{"key":"e_1_3_2_10_2","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1016\/j.virol.2005.08.042","volume":"345","year":"2006","unstructured":"Haynes, B. F., B. Ma, D. C. Montefiori, T. Wrin, C. J. Petropoulos, L. L. Sutherland, R. M. Scearce, C. Denton, S. M. Xia, B. T. Korber, and H. X. Liao. 2006. Analysis of HIV-1 subtype B third variable region peptide motifs for induction of neutralizing antibodies against HIV-1 primary isolates. Virology345:44-55.","journal-title":"Virology"},{"key":"e_1_3_2_11_2","doi-asserted-by":"crossref","first-page":"5299","DOI":"10.1073\/pnas.95.9.5299","volume":"95","year":"1998","unstructured":"Hess, J., D. Miko, A. Catic, V. Lehmensiek, D. G. Russell, and S. H. Kaufmann. 1998. Mycobacterium bovis Bacille Calmette-Guerin strains secreting listeriolysin of Listeria monocytogenes. Proc. Natl. Acad. Sci. USA95:5299-5304.","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"e_1_3_2_12_2","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0065-2776(00)75001-2","volume":"75","year":"2000","unstructured":"Hess, J., U. Schaible, B. Raupach, and S. H. Kaufmann. 2000. Exploiting the immune system: toward new vaccines against intracellular bacteria. Adv. Immunol.75:1-88.","journal-title":"Adv. Immunol."},{"key":"e_1_3_2_13_2","doi-asserted-by":"crossref","first-page":"5862","DOI":"10.4049\/jimmunol.167.10.5862","volume":"167","year":"2001","unstructured":"Hiroi, T., H. Goto, K. Someya, M. Yanagita, M. Honda, N. Yamanaka, and H. Kiyono. 2001. HIV mucosal vaccine: nasal immunization with rBCG-V3J1 induces a long term V3J1 peptide-specific neutralizing immunity in Th1- and Th2-deficient conditions. J. Immunol.167:5862-5867.","journal-title":"J. Immunol."},{"key":"e_1_3_2_14_2","doi-asserted-by":"crossref","first-page":"2199","DOI":"10.1084\/jem.188.12.2199","volume":"188","year":"1998","unstructured":"Kalams, S. A., and B. D. Walker. 1998. The critical need for CD4 help in maintaining effective cytotoxic T lymphocyte responses. J. Exp. Med.188:2199-2204.","journal-title":"J. Exp. Med."},{"key":"e_1_3_2_15_2","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1016\/S0165-2478(98)00128-X","volume":"65","year":"1999","unstructured":"Kaufmann, S. H., and J. Hess. 1999. Impact of intracellular location of and antigen display by intracellular bacteria: implications for vaccine development. Immunol. Lett.65:81-84.","journal-title":"Immunol. Lett."},{"key":"e_1_3_2_16_2","doi-asserted-by":"crossref","first-page":"326","DOI":"10.1006\/clim.2002.5292","volume":"105","year":"2002","unstructured":"Kawahara, M., A. Hashimoto, I. Toida, and M. Honda. 2002. Oral recombinant Mycobacterium bovis bacillus Calmette-Guerin expressing HIV-1 antigens as a freeze-dried vaccine induces long-term, HIV-specific mucosal and systemic immunity. Clin. Immunol.105:326-331.","journal-title":"Clin. Immunol."},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2006.04.043"},{"key":"e_1_3_2_18_2","doi-asserted-by":"crossref","first-page":"939","DOI":"10.1016\/j.micinf.2005.03.012","volume":"7","year":"2005","unstructured":"Nasser Eddine, A., and S. H. Kaufmann. 2005. Improved protection by recombinant BCG. Microbes Infect.7:939-946.","journal-title":"Microbes Infect."},{"key":"e_1_3_2_19_2","doi-asserted-by":"crossref","first-page":"451","DOI":"10.1016\/j.coi.2004.05.004","volume":"16","year":"2004","unstructured":"Northrop, J. K., and H. Shen. 2004. CD8+ T-cell memory: only the good ones last. Curr. Opin. Immunol.16:451-455.","journal-title":"Curr. Opin. Immunol."},{"key":"e_1_3_2_20_2","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1146\/annurev.immunol.16.1.323","volume":"16","year":"1998","unstructured":"Pamer, E., and P. Cresswell. 1998. Mechanisms of MHC class I-restricted antigen processing. Annu. Rev. Immunol.16:323-358.","journal-title":"Annu. Rev. Immunol."},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.78.23.13163-13172.2004"},{"key":"e_1_3_2_22_2","unstructured":"Molecular cloning: a laboratory manual 1989"},{"key":"e_1_3_2_23_2","doi-asserted-by":"crossref","first-page":"1039","DOI":"10.1038\/nm906","volume":"9","year":"2003","unstructured":"Schaible, U. E., F. Winau, P. A. Sieling, K. Fischer, H. L. Collins, K. Hagens, R. L. Modlin, V. Brinkmann, and S. H. Kaufmann. 2003. Apoptosis facilitates antigen presentation to T lymphocytes through MHC-I and CD1 in tuberculosis. Nat. Med.9:1039-1046.","journal-title":"Nat. Med."},{"key":"e_1_3_2_24_2","doi-asserted-by":"crossref","first-page":"6991","DOI":"10.4049\/jimmunol.167.12.6991","volume":"167","year":"2001","unstructured":"Serbina, N. V., V. Lazarevic, and J. L. Flynn. 2001. CD4(+) T cells are required for the development of cytotoxic CD8(+) T cells during Mycobacterium tuberculosis infection. J. Immunol.167:6991-7000.","journal-title":"J. Immunol."},{"key":"e_1_3_2_25_2","doi-asserted-by":"crossref","first-page":"1911","DOI":"10.1111\/j.1365-2958.1990.tb02040.x","volume":"4","year":"1990","unstructured":"Snapper, S. B., R. E. Melton, S. Mustafa, T. Kieser, and W. R. Jacobs, Jr. 1990. Isolation and characterization of efficient plasmid transformation mutants of Mycobacterium smegmatis. Mol. Microbiol.4:1911-1919.","journal-title":"Mol. Microbiol."},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.79.3.1452-1462.2005"},{"key":"e_1_3_2_27_2","doi-asserted-by":"crossref","first-page":"456","DOI":"10.1038\/351456a0","volume":"351","year":"1991","unstructured":"Stover, C. K., V. F. de la Cruz, T. R. Fuerst, J. E. Burlein, L. A. Benson, L. T. Bennett, G. P. Bansal, J. F. Young, M. H. Lee, G. F. Hatfull, et al. 1991. New use of BCG for recombinant vaccines. Nature351:456-460.","journal-title":"Nature"},{"key":"e_1_3_2_28_2","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1111\/j.1574-6968.1996.tb08563.x","volume":"145","year":"1996","unstructured":"Wards, B. J., and D. M. Collins. 1996. Electroporation at elevated temperatures substantially improves transformation efficiency of slow-growing mycobacteria. FEMS Microbiol. Lett.145:101-105.","journal-title":"FEMS Microbiol. Lett."},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02484-05"},{"key":"e_1_3_2_30_2","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/j.immuni.2005.12.001","volume":"24","year":"2006","unstructured":"Winau, F., S. Weber, S. Sad, J. de Diego, S. L. Hoops, B. Breiden, K. Sandhoff, V. Brinkmann, S. H. Kaufmann, and U. E. Schaible. 2006. Apoptotic vesicles crossprime CD8 T cells and protect against tuberculosis. Immunity24:105-117.","journal-title":"Immunity"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00195-06"}],"container-title":["Clinical and Vaccine Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/CVI.00407-06","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/CVI.00407-06","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,7,29]],"date-time":"2021-07-29T20:38:44Z","timestamp":1627591124000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/CVI.00407-06"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,7]]},"references-count":30,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2007,7]]}},"alternative-id":["10.1128\/CVI.00407-06"],"URL":"https:\/\/doi.org\/10.1128\/cvi.00407-06","relation":{},"ISSN":["1556-6811","1556-679X"],"issn-type":[{"value":"1556-6811","type":"print"},{"value":"1556-679X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,7]]},"assertion":[{"value":"2006-10-02","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2007-05-09","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-12-18","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}