{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T13:26:04Z","timestamp":1787318764113,"version":"build-2736575974"},"reference-count":7,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2009,3,1]],"date-time":"2009-03-01T00:00:00Z","timestamp":1235865600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Vaccine Immunol"],"published-print":{"date-parts":[[2009,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The sensitivity for detection of\n                    <jats:italic>Histoplasma<\/jats:italic>\n                    antigen is lower in serum than in urine. In other antigen assays, treatment of serum at 104\u00b0C in the presence of EDTA was required for detection of antigenemia. Sensitivity and specificity for detection of\n                    <jats:italic>Histoplasma<\/jats:italic>\n                    antigenemia were examined with or without EDTA heat treatment of the serum using the MVista\n                    <jats:italic>Histoplasma<\/jats:italic>\n                    antigen enzyme immunoassay. A total of 94.6% of serum specimens from patients with AIDS and histoplasmosis that were negative untreated were positive after EDTA-heat treatment. Two-thirds of the negative serum specimens from patients with probable histoplasmosis, based upon clinical suspicion and\n                    <jats:italic>Histoplasma<\/jats:italic>\n                    antigenuria, were positive after heat treatment. Specificity was 99.0% in controls, including healthy subjects and patients in whom histoplasmosis or blastomycosis, were excluded. Precision and reproducibility were good and excellent, respectively. These findings demonstrate improvement in sensitivity without reduction in specificity, precision, or reproducibility after heat-EDTA treatment.\n                  <\/jats:p>","DOI":"10.1128\/cvi.00409-08","type":"journal-article","created":{"date-parts":[[2009,1,14]],"date-time":"2009-01-14T22:41:51Z","timestamp":1231972911000},"page":"320-322","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":58,"title":["Improved Detection of\n                    <i>Histoplasma<\/i>\n                    Antigenemia following Dissociation of Immune Complexes"],"prefix":"10.1128","volume":"16","author":[{"given":"S.","family":"Swartzentruber","sequence":"first","affiliation":[{"name":"MiraVista Diagnostics, Indianapolis, Indiana"},{"name":"Indiana University-Department of Pathology and Laboratory Medicine, Indianapolis, Indiana"},{"name":"Roudebush Veterans' Administration Medical Center, Indianapolis, Indiana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"LeMonte","sequence":"additional","affiliation":[{"name":"MiraVista Diagnostics, Indianapolis, Indiana"},{"name":"Indiana University-Department of Pathology and Laboratory Medicine, Indianapolis, Indiana"},{"name":"Roudebush Veterans' Administration Medical Center, Indianapolis, Indiana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Witt","sequence":"additional","affiliation":[{"name":"MiraVista Diagnostics, Indianapolis, Indiana"},{"name":"Indiana University-Department of Pathology and Laboratory Medicine, Indianapolis, Indiana"},{"name":"Roudebush Veterans' Administration Medical Center, Indianapolis, Indiana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.","family":"Fuller","sequence":"additional","affiliation":[{"name":"MiraVista Diagnostics, Indianapolis, Indiana"},{"name":"Indiana University-Department of Pathology and Laboratory Medicine, Indianapolis, Indiana"},{"name":"Roudebush Veterans' Administration Medical Center, Indianapolis, Indiana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.","family":"Davis","sequence":"additional","affiliation":[{"name":"MiraVista Diagnostics, Indianapolis, Indiana"},{"name":"Indiana University-Department of Pathology and Laboratory Medicine, Indianapolis, Indiana"},{"name":"Roudebush Veterans' Administration Medical Center, Indianapolis, Indiana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Hage","sequence":"additional","affiliation":[{"name":"MiraVista Diagnostics, Indianapolis, Indiana"},{"name":"Indiana University-Department of Pathology and Laboratory Medicine, Indianapolis, Indiana"},{"name":"Roudebush Veterans' Administration Medical Center, Indianapolis, Indiana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Connolly","sequence":"additional","affiliation":[{"name":"MiraVista Diagnostics, Indianapolis, Indiana"},{"name":"Indiana University-Department of Pathology and Laboratory Medicine, Indianapolis, Indiana"},{"name":"Roudebush Veterans' Administration Medical Center, Indianapolis, Indiana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Durkin","sequence":"additional","affiliation":[{"name":"MiraVista Diagnostics, Indianapolis, Indiana"},{"name":"Indiana University-Department of Pathology and Laboratory Medicine, Indianapolis, Indiana"},{"name":"Roudebush Veterans' Administration Medical Center, Indianapolis, Indiana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L. J.","family":"Wheat","sequence":"additional","affiliation":[{"name":"MiraVista Diagnostics, Indianapolis, Indiana"},{"name":"Indiana University-Department of Pathology and Laboratory Medicine, Indianapolis, Indiana"},{"name":"Roudebush Veterans' Administration Medical Center, Indianapolis, Indiana"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00071-07"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00493-07"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00358-07"},{"key":"e_1_3_2_5_2","doi-asserted-by":"crossref","first-page":"105","DOI":"10.7326\/0003-4819-137-2-200207160-00008","volume":"137","year":"2002","unstructured":"Johnson, P. C., L. J. Wheat, G. A. Cloud, M. Goldman, D. Lancaster, D. M. Bamberger, W. G. Powderly, R. Hafner, C. A. Kauffman, and W. E. Dismukes. 2002. Safety and efficacy of liposomal amphotericin B compared with conventional amphotericin B for induction therapy of histoplasmosis in patients with AIDS. Ann. Intern. Med.137:105-109.","journal-title":"Ann. Intern. Med."},{"key":"e_1_3_2_6_2","doi-asserted-by":"crossref","first-page":"336","DOI":"10.1016\/S0002-9343(99)80311-8","volume":"98","year":"1995","unstructured":"Wheat, J., R. Hafner, A. H. Korzun, M. T. Limjoco, P. Spencer, R. A. Larsen, F. M. Hecht, W. Powderly, et al. 1995. Itraconazole treatment of disseminated histoplasmosis in patients with the acquired immunodeficiency syndrome. Am. J. Med.98:336-342.","journal-title":"Am. J. Med."},{"key":"e_1_3_2_7_2","doi-asserted-by":"crossref","first-page":"807","DOI":"10.1086\/521259","volume":"45","year":"2007","unstructured":"Wheat, L. J., A. G. Freifeld, M. B. Kleiman, J. W. Baddley, D. S. McKinsey, J. E. Loyd, and C. A. Kauffman. 2007. Clinical practice guidelines for the management of patients with histoplasmosis: 2007 update by the Infectious Diseases Society of America. Clin. Infect. Dis.45:807-825.","journal-title":"Clin. Infect. Dis."},{"key":"e_1_3_2_8_2","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1007\/s10096-007-0437-7","volume":"27","year":"2008","unstructured":"Wheat, L. J., and T. J. Walsh. 2008. Diagnosis of invasive aspergillosis by galactomannan antigenemia detection using an enzyme immunoassay. Eur. J. Clin. Microbiol. Infect. Dis.27:245-251.","journal-title":"Eur. J. Clin. Microbiol. Infect. Dis."}],"container-title":["Clinical and Vaccine Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/CVI.00409-08","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/CVI.00409-08","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,7,29]],"date-time":"2021-07-29T16:41:48Z","timestamp":1627576908000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/CVI.00409-08"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,3]]},"references-count":7,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2009,3]]}},"alternative-id":["10.1128\/CVI.00409-08"],"URL":"https:\/\/doi.org\/10.1128\/cvi.00409-08","relation":{},"ISSN":["1556-6811","1556-679X"],"issn-type":[{"value":"1556-6811","type":"print"},{"value":"1556-679X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,3]]},"assertion":[{"value":"2008-11-08","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2009-01-02","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-12-18","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}