{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T04:14:11Z","timestamp":1777608851566,"version":"3.51.4"},"reference-count":51,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2010,3,1]],"date-time":"2010-03-01T00:00:00Z","timestamp":1267401600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Vaccine Immunol"],"published-print":{"date-parts":[[2010,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>We are developing a<jats:italic>Salmonella<\/jats:italic>vectored vaccine to prevent infant pneumonia and other diseases caused by<jats:italic>Streptococcus pneumoniae<\/jats:italic>. One prerequisite for achieving this goal is to construct and evaluate new recombinant attenuated<jats:italic>Salmonella<\/jats:italic>vaccine (RASV) strains suitable for use in neonates and infants.<jats:italic>Salmonella enterica<\/jats:italic>serovar Typhimurium strain \u03c79558(pYA4088) specifies delivery of the pneumococcal protective antigen PspA and can protect adult mice from challenge with<jats:italic>S. pneumoniae<\/jats:italic>. This strain is completely safe for oral delivery to day-old and infant mice. Here we assess the colonizing ability, immunogenicity, and protective efficacy of \u03c79558(pYA4088) in neonatal mice. Colonization was assessed in mice 0, 2, 4, or 7 days of age after oral inoculation. In the presence of maternal antibodies, the colonization of lymphoid tissues was delayed, but the immune responses were enhanced in mice born to immunized mothers. Both oral and intranasal routes were used to assess immunogenicity. All orally or intranasally immunized neonatal and infant mice born to either immunized or nai\u0308ve mothers developed PspA-specific mucosal and systemic immune responses. Mice born to immunized mothers produced higher titers of PspA-specific antibodies in the blood and mucosa and greater numbers of PspA-specific interleukin-4 (IL-4)-secreting cells than mice born to nai\u0308ve mothers. More importantly, mice born to immune mothers showed a significant increase in protection against<jats:italic>S. pneumoniae<\/jats:italic>challenge. These results suggest that strain \u03c79558(pYA4088) can circumvent some of the limitations of the immature immune system in neonatal and infant mice, generating enhanced protective immune responses in the presence of maternal antibodies.<\/jats:p>","DOI":"10.1128\/cvi.00413-09","type":"journal-article","created":{"date-parts":[[2010,1,7]],"date-time":"2010-01-07T03:05:03Z","timestamp":1262833503000},"page":"363-371","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":31,"title":["Immunogenicity of a Live Recombinant<i>Salmonella<\/i><i>enterica<\/i>Serovar Typhimurium Vaccine Expressing<i>pspA<\/i>in Neonates and Infant Mice Born from Nai\u0308ve and Immunized Mothers"],"prefix":"10.1128","volume":"17","author":[{"given":"Huoying","family":"Shi","sequence":"first","affiliation":[{"name":"The Biodesign Institute"},{"name":"School of Life Sciences, Arizona State University, Tempe, Arizona 85287"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shifeng","family":"Wang","sequence":"additional","affiliation":[{"name":"The Biodesign Institute"},{"name":"School of Life Sciences, Arizona State University, Tempe, Arizona 85287"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kenneth L.","family":"Roland","sequence":"additional","affiliation":[{"name":"The Biodesign Institute"},{"name":"School of Life Sciences, Arizona State University, Tempe, Arizona 85287"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bronwyn M.","family":"Gunn","sequence":"additional","affiliation":[{"name":"The Biodesign Institute"},{"name":"School of Life Sciences, Arizona State University, Tempe, Arizona 85287"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"III","given":"Roy","family":"Curtiss","sequence":"additional","affiliation":[{"name":"The Biodesign Institute"},{"name":"School of Life Sciences, Arizona State University, Tempe, Arizona 85287"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1093\/clinids\/15.1.77","volume":"15","year":"1992","unstructured":"Appelbaum, P. 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