{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T17:46:22Z","timestamp":1793123182628,"version":"4.5.0"},"reference-count":15,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2009,4,1]],"date-time":"2009-04-01T00:00:00Z","timestamp":1238544000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Vaccine Immunol"],"published-print":{"date-parts":[[2009,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Vaccination is a tool that could be beneficial in managing the high prevalence of brucellosis in free-ranging bison in Yellowstone National Park. In this study, we characterized immunologic responses and protection against experimental challenge after vaccination of bison with<jats:italic>Brucella abortus<\/jats:italic>strain RB51 (RB51) or a recombinant RB51 strain overexpressing superoxide dismutase (<jats:italic>sodC<\/jats:italic>) and glycosyltransferase (<jats:italic>wboA<\/jats:italic>) genes (RB51+<jats:italic>sodC<\/jats:italic>,<jats:italic>wboA<\/jats:italic>). Bison were vaccinated with saline only or with 4.6 \u00d7 10<jats:sup>10<\/jats:sup>CFU of RB51 or 7.4 \u00d7 10<jats:sup>10<\/jats:sup>CFU of RB51+<jats:italic>sodC<\/jats:italic>,<jats:italic>wboA<\/jats:italic>(<jats:italic>n<\/jats:italic>= eight animals\/treatment). Bison vaccinated with RB51 or RB51+<jats:italic>sodC<\/jats:italic>,<jats:italic>wboA<\/jats:italic>had greater (<jats:italic>P<\/jats:italic>&lt; 0.05) antibody responses, proliferative responses, and production of gamma interferon to RB51 after vaccination than did nonvaccinates. However, bison vaccinated with RB51+<jats:italic>sodC<\/jats:italic>,<jats:italic>wboA<\/jats:italic>cleared the vaccine strain from draining lymph nodes faster than bison vaccinated with the parental RB51 strain. Immunologic responses of bison vaccinated with RB51+<jats:italic>sodC<\/jats:italic>,<jats:italic>wboA<\/jats:italic>were similar to responses of bison vaccinated with RB51. Pregnant bison were intraconjunctivally challenged in midgestation with 10<jats:sup>7<\/jats:sup>CFU of<jats:italic>B. abortus<\/jats:italic>strain 2308. Bison vaccinated with RB51, but not RB51+<jats:italic>sodC<\/jats:italic>,<jats:italic>wboA<\/jats:italic>vaccinates, had greater protection from abortion, fetal\/uterine, mammary, or maternal infection than nonvaccinates. Our data suggest that the RB51+<jats:italic>sodC<\/jats:italic>,<jats:italic>wboA<\/jats:italic>strain is less efficacious as a calfhood vaccine for bison than the parental RB51 strain. Our data also suggest that the RB51 vaccine is a currently available management tool that could be utilized to help reduce brucellosis in free-ranging bison.<\/jats:p>","DOI":"10.1128\/cvi.00419-08","type":"journal-article","created":{"date-parts":[[2009,1,29]],"date-time":"2009-01-29T01:58:59Z","timestamp":1233194339000},"page":"535-540","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":49,"title":["Immune Responses and Protection against Experimental Challenge after Vaccination of Bison with<i>Brucella abortus<\/i>Strain RB51 or RB51 Overexpressing Superoxide Dismutase and Glycosyltransferase Genes"],"prefix":"10.1128","volume":"16","author":[{"given":"S. C.","family":"Olsen","sequence":"first","affiliation":[{"name":"Bacterial Diseases of Livestock Research Unit, National Animal Disease Center, Agricultural Research Service, U.S. Department of Agriculture, Ames, Iowa 50010"},{"name":"Department of Biomedical Sciences and Pathobiology, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Tech, Blacksburg, Virginia 24061"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S. M.","family":"Boyle","sequence":"additional","affiliation":[{"name":"Bacterial Diseases of Livestock Research Unit, National Animal Disease Center, Agricultural Research Service, U.S. Department of Agriculture, Ames, Iowa 50010"},{"name":"Department of Biomedical Sciences and Pathobiology, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Tech, Blacksburg, Virginia 24061"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G. G.","family":"Schurig","sequence":"additional","affiliation":[{"name":"Bacterial Diseases of Livestock Research Unit, National Animal Disease Center, Agricultural Research Service, U.S. Department of Agriculture, Ames, Iowa 50010"},{"name":"Department of Biomedical Sciences and Pathobiology, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Tech, Blacksburg, Virginia 24061"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N. N.","family":"Sriranganathan","sequence":"additional","affiliation":[{"name":"Bacterial Diseases of Livestock Research Unit, National Animal Disease Center, Agricultural Research Service, U.S. Department of Agriculture, Ames, Iowa 50010"},{"name":"Department of Biomedical Sciences and Pathobiology, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Tech, Blacksburg, Virginia 24061"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"17","year":"1988","unstructured":"Alton, G. G., L. M. Jones, R. D. Angus, and J. M. Verger. 1988. Bacteriological methods serological methods, p. 17-136. InTechniques for the brucellosis laboratory. Institut National de la Recherche Agronomique, Paris, France.","journal-title":"Techniques for the brucellosis laboratory."},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.58.9.2935-2939.1990"},{"key":"e_1_3_2_4_2","doi-asserted-by":"crossref","first-page":"1881","DOI":"10.2460\/ajvr.1992.53.10.1881","volume":"53","year":"1992","unstructured":"Cheville, N. F., A. E. Jensen, S. M. Halling, F. M. Tatum, D. C. Morfitt, S. G. Hennager, W. M. Frerichs, and G. Schurig. 1992. Bacterial survival, lymph node changes and immunologic responses of cattle vaccinated with standard and mutant strains of Brucella abortus. Am. J. Vet. Res.53:1881-1888.","journal-title":"Am. J. Vet. Res."},{"key":"e_1_3_2_5_2","doi-asserted-by":"crossref","first-page":"1153","DOI":"10.2460\/ajvr.1996.57.08.1153","volume":"57","year":"1996","unstructured":"Cheville, N. F., S. C. Olsen, A. E. Jensen, M. G. Stevens, and M. V. Palmer. 1996. Effects of age at vaccination on efficacy of Brucella abortus strain RB51 to protect cattle against brucellosis. Am. J. Vet. Res.57:1153-1156.","journal-title":"Am. J. Vet. Res."},{"key":"e_1_3_2_6_2","first-page":"347","year":"1993","unstructured":"Davis, D. S. 1993. Summary of bison\/brucellosis research conducted at Texas A&M University 1985-1993, p. 347-361. In R. E. Walker (ed.), Proceedings of North American Public Bison Herds Symposium. National Bison Association, Denver, CO.","journal-title":"Proceedings of North American Public Bison Herds Symposium."},{"key":"e_1_3_2_7_2","doi-asserted-by":"crossref","first-page":"27","DOI":"10.7589\/0090-3558-38.1.27","volume":"38","year":"2002","unstructured":"Kreeger, T. J., W. E. Cook, W. H. Edwards, P. H. Elzer, and S. C. Olsen. 2002. Brucella abortus strain RB51 vaccination in elk. II. Failure of high dosage to prevent abortion. J. Wildl. Dis.38:27-31.","journal-title":"J. Wildl. Dis."},{"key":"e_1_3_2_8_2","first-page":"223","volume":"65","year":"2001","unstructured":"Lee, L.-K., S. C. Olsen, and C. A. Bolin. 2001. Effects of exogenous recombinant interleukin-12 on immune responses and protection against Brucella abortus in a murine model. Can. J. Vet. Res.65:223-228.","journal-title":"Can. J. Vet. Res."},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00213-06"},{"key":"e_1_3_2_10_2","first-page":"17","volume":"74","year":"2003","unstructured":"Olsen, S. C., A. E. Jensen, W. C. Stoffregen, and M. V. Palmer. 2003. Efficacy of calfhood vaccination with Brucella abortus strain RB51 in protecting bison against brucellosis. Res. Vet. Sci.74:17-22.","journal-title":"Res. Vet. Sci."},{"key":"e_1_3_2_11_2","doi-asserted-by":"crossref","first-page":"738","DOI":"10.7589\/0090-3558-38.4.738","volume":"38","year":"2002","unstructured":"Olsen, S. C., T. J. Kreeger, and W. Schultz. 2002. Immune responses of bison to ballistic or hand vaccination with Brucella abortus strain RB51. J. Wildl. Dis.38:738-745.","journal-title":"J. Wildl. Dis."},{"key":"e_1_3_2_12_2","first-page":"359","volume":"26","year":"1990","unstructured":"Sriranganathan, N., S. M. Boyle, G. G. Schurig, and H. Misra. 1990. Superoxide dismutases of virulent and avirulent strains of Brucella abortus. Vet. Microbiol.26:359-366.","journal-title":"Vet. Microbiol."},{"key":"e_1_3_2_13_2","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1016\/j.vetmic.2004.07.001","volume":"102","year":"2004","unstructured":"Vemulapalli, R., A. Contreras, N. Sanakkayala, N. Sriranganathan, S. M. Boyle, and G. G. Schurig. 2004. Enhanced efficacy of recombinant Brucella abortus RB51 vaccines against B. melitensis infection in mice. Vet. Microbiol.102:237-245.","journal-title":"Vet. 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