{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T18:36:20Z","timestamp":1789583780756,"version":"build-2803163510"},"reference-count":36,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2007,3,1]],"date-time":"2007-03-01T00:00:00Z","timestamp":1172707200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Vaccine Immunol"],"published-print":{"date-parts":[[2007,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            An enzyme-linked immunosorbent assay (ELISA) was developed for specific antibody detection in serum specimens of patients with sporotrichosis. The assay was made with mycelial-phase\n            <jats:italic>Sporothrix schenckii<\/jats:italic>\n            exoantigens and was tested against 90 sera from patients with different clinical forms of sporotrichosis. Potential cross-reactions were analyzed with 72 heterologous sera from patients with paracoccidioidomycosis, cryptococcosis, aspergillosis, histoplasmosis, tuberculosis, and American tegumentary leishmaniasis, as well as 76 sera from healthy controls. We found a sensitivity of 97% and a specificity of 89% in this assay. Some cross-reactions were seen, as observed in other immunoassays for the diagnosis of sporotrichosis. The ELISA appears to be especially useful for cutaneous forms of disease, since these are not promptly diagnosed with available immunoprecipitation or agglutination techniques. These results suggest that the ELISA using mycelial-phase\n            <jats:italic>S. schenckii<\/jats:italic>\n            exoantigens is a very sensitive diagnostic tool for the serodiagnosis of sporotrichosis and can be used in conjunction with conventional methods of diagnosis, particularly in cases where cross-reactions or false-positive results are experienced with the serodiagnosis.\n          <\/jats:p>","DOI":"10.1128\/cvi.00430-06","type":"journal-article","created":{"date-parts":[[2007,1,11]],"date-time":"2007-01-11T01:39:00Z","timestamp":1168479540000},"page":"244-249","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":55,"title":["Use of Mycelial-Phase\n            <i>Sporothrix schenckii<\/i>\n            Exoantigens in an Enzyme-Linked Immunosorbent Assay for Diagnosis of Sporotrichosis by Antibody Detection"],"prefix":"10.1128","volume":"14","author":[{"given":"Rodrigo","family":"Almeida-Paes","sequence":"first","affiliation":[{"name":"Servic\u0327o de Micologia, Instituto de Pesquisa Cli\u0301nica Evandro Chagas, Fiocruz, Rio de Janeiro, Brazil"},{"name":"Departamento de Imunologia, Instituto de Microbiologia Professor Paulo de Go\u0301es, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil"},{"name":"Division of Infectious Diseases, Department of Medicine"},{"name":"Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, New York"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monique A.","family":"Pimenta","sequence":"additional","affiliation":[{"name":"Servic\u0327o de Micologia, Instituto de Pesquisa Cli\u0301nica Evandro Chagas, Fiocruz, Rio de Janeiro, Brazil"},{"name":"Departamento de Imunologia, Instituto de Microbiologia Professor Paulo de Go\u0301es, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil"},{"name":"Division of Infectious Diseases, Department of Medicine"},{"name":"Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, New York"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudia Vera","family":"Pizzini","sequence":"additional","affiliation":[{"name":"Servic\u0327o de Micologia, Instituto de Pesquisa Cli\u0301nica Evandro Chagas, Fiocruz, Rio de Janeiro, Brazil"},{"name":"Departamento de Imunologia, Instituto de Microbiologia Professor Paulo de Go\u0301es, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil"},{"name":"Division of Infectious Diseases, Department of Medicine"},{"name":"Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, New York"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paulo Cezar F.","family":"Monteiro","sequence":"additional","affiliation":[{"name":"Servic\u0327o de Micologia, Instituto de Pesquisa Cli\u0301nica Evandro Chagas, Fiocruz, Rio de Janeiro, Brazil"},{"name":"Departamento de Imunologia, Instituto de Microbiologia Professor Paulo de Go\u0301es, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil"},{"name":"Division of Infectious Diseases, Department of Medicine"},{"name":"Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, New York"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jose\u0301 Mauro","family":"Peralta","sequence":"additional","affiliation":[{"name":"Servic\u0327o de Micologia, Instituto de Pesquisa Cli\u0301nica Evandro Chagas, Fiocruz, Rio de Janeiro, Brazil"},{"name":"Departamento de Imunologia, Instituto de Microbiologia Professor Paulo de Go\u0301es, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil"},{"name":"Division of Infectious Diseases, Department of Medicine"},{"name":"Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, New 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