{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T10:16:28Z","timestamp":1790417788978,"version":"4.1.0"},"reference-count":54,"publisher":"American Society for Microbiology","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clin. Vaccine Immunol."],"published-print":{"date-parts":[[2014,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:named-content content-type=\"genus-species\">Helicobacter pylori<\/jats:named-content>\n                    infection is relatively common worldwide and is closely related to gastric mucosa-associated lymphoid tissue (MALT) lymphoma, chronic gastritis, and stomach ulcers. Therefore, a safe and effective method for preventing\n                    <jats:named-content content-type=\"genus-species\">H. pylori<\/jats:named-content>\n                    infection is urgently needed. Given that developing an effective vaccine against\n                    <jats:named-content content-type=\"genus-species\">H. pylori<\/jats:named-content>\n                    is one of the best alternatives,\n                    <jats:named-content content-type=\"genus-species\">H. pylori<\/jats:named-content>\n                    adhesin Hp0410 was expressed in the food-grade bacterium\n                    <jats:named-content content-type=\"genus-species\">Lactobacillus acidophilus<\/jats:named-content>\n                    . The recombinant live bacterial vaccine was then used to orally vaccinate mice, and the immunoprotective effects of Hp0410-producing strains were investigated.\n                    <jats:named-content content-type=\"genus-species\">H. pylori<\/jats:named-content>\n                    colonization in the stomach of mice immunized with the recombinant\n                    <jats:named-content content-type=\"genus-species\">L. acidophilus<\/jats:named-content>\n                    was significantly reduced, in comparison with that in control groups. Furthermore, mucosal secretory IgA antibodies were elicited in the mucosal tissue of mice immunized with the recombinant bacteria, and specific anti-Hp0410 IgG responses were also detected in mouse serum. There was a significant increase in the level of protection against gastric\n                    <jats:named-content content-type=\"genus-species\">Helicobacter<\/jats:named-content>\n                    infection following a challenge with\n                    <jats:named-content content-type=\"genus-species\">H. pylori<\/jats:named-content>\n                    Sydney strain 1 (SS1). Our results collectively indicate that adhesin Hp0410 is a promising candidate vaccine antigen, and recombinant\n                    <jats:named-content content-type=\"genus-species\">L. acidophilus<\/jats:named-content>\n                    expressing Hp0410 is likely to constitute an effective, low-cost, live bacterial vaccine against\n                    <jats:named-content content-type=\"genus-species\">H. pylori<\/jats:named-content>\n                    .\n                  <\/jats:p>","DOI":"10.1128\/cvi.00434-13","type":"journal-article","created":{"date-parts":[[2013,11,27]],"date-time":"2013-11-27T21:38:14Z","timestamp":1385588294000},"page":"126-132","source":"Crossref","is-referenced-by-count":41,"title":["Oral Immunization with Recombinant Lactobacillus acidophilus Expressing the Adhesin Hp0410 of Helicobacter pylori Induces Mucosal and Systemic Immune Responses"],"prefix":"10.1128","volume":"21","author":[{"given":"Fan","family":"Hongying","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wu","family":"Xianbo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Fang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bai","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Long","family":"Beiguo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","published-online":{"date-parts":[[2013,11,27]]},"reference":[{"key":"key20180919073720_B1","doi-asserted-by":"publisher","first-page":"693","DOI":"10.1016\/S1286-4579(03)00111-4","article-title":"Burden of Helicobacter pylori and H. pylori related diseases in developed countries: recent developments and future implications","volume":"5","author":"Rothenbacher","year":"2003","journal-title":"Microbes Infect."},{"key":"key20180919073720_B2","doi-asserted-by":"publisher","first-page":"299","DOI":"10.1097\/MCG.0b013e31820fb8f6","article-title":"Helicobacter pylori in developing countries: World Gastroenterology Organisation Global Guideline","volume":"20","author":"Hunt","year":"2011","journal-title":"J. Gastrointestin. Liver Dis."},{"key":"key20180919073720_B3","doi-asserted-by":"publisher","first-page":"931","DOI":"10.1016\/S0140-6736(02)08025-X","article-title":"Age at acquisition of Helicobacter pylori infection: a follow-up study from infancy to adulthood","volume":"359","author":"Malaty","year":"2002","journal-title":"Lancet"},{"key":"key20180919073720_B4","doi-asserted-by":"publisher","first-page":"674","DOI":"10.1097\/00006454-200207000-00014","article-title":"Role of infected parents in transmission of Helicobacter pylori to their children","volume":"21","author":"Rothenbacher","year":"2002","journal-title":"Pediatr. Infect. Dis. J."},{"key":"key20180919073720_B5","doi-asserted-by":"publisher","first-page":"2240","DOI":"10.1001\/jama.282.23.2240","article-title":"Fecal and oral shedding of Helicobacter pylori from healthy infected adults","volume":"282","author":"Parsonnet","year":"1999","journal-title":"JAMA"},{"key":"key20180919073720_B6","doi-asserted-by":"publisher","first-page":"36","DOI":"10.1136\/mp.56.1.36","article-title":"Helicobacter pylori genotyping in gastric adenocarcinoma and MALT lymphoma by multiplex PCR analyses of paraffin wax embedded tissues","volume":"56","author":"Koehler","year":"2003","journal-title":"Mol. Pathol."},{"key":"key20180919073720_B7","doi-asserted-by":"publisher","first-page":"308","DOI":"10.5009\/gnl.2011.5.3.308","article-title":"Role of F-FDG PET scans in patients with Helicobacter pylori-infected gastric low-grade MALT lymphoma","volume":"5","author":"Song","year":"2011","journal-title":"Gut Liver"},{"key":"key20180919073720_B8","first-page":"1","article-title":"Schistosomes, liver flukes and Helicobacter pylori","volume":"61","author":"IARC","year":"1994","journal-title":"IARC Monogr. Eval. Carcinog. Risks Hum."},{"key":"key20180919073720_B9","doi-asserted-by":"publisher","first-page":"1047","DOI":"10.1111\/j.1365-2036.2005.02408.x","article-title":"Long-term follow-up of Helicobacter pylori eradication therapy in Vietnam: reinfection and clinical outcome","volume":"21","author":"Wheeldon","year":"2005","journal-title":"Aliment. Pharmacol. Ther."},{"key":"key20180919073720_B10","doi-asserted-by":"publisher","first-page":"388","DOI":"10.1046\/j.1469-0691.2002.00435.x","article-title":"The status of antimicrobial resistance of Helicobacter pylori in Eastern Europe","volume":"8","author":"Boyanova","year":"2002","journal-title":"Clin. Microbiol. Infect."},{"key":"key20180919073720_B11","doi-asserted-by":"publisher","first-page":"705","DOI":"10.1016\/S1286-4579(03)00112-6","article-title":"Helicobacter pylori in the developing world","volume":"5","author":"Frenck","year":"2003","journal-title":"Microbes Infect."},{"key":"key20180919073720_B12","doi-asserted-by":"publisher","first-page":"2083","DOI":"10.1111\/j.1572-0241.2005.50043.x","article-title":"The recurrence of Helicobacter pylori infection: incidence and variables influencing it: a critical review","volume":"100","author":"Gisbert","year":"2005","journal-title":"Am. J. Gastroenterol."},{"key":"key20180919073720_B13","first-page":"561","article-title":"Detection and analysis of Helicobacter pylori in oral cavity and stomach","volume":"10","author":"Zhu","year":"2008","journal-title":"J. Oral Sci. Res. China"},{"key":"key20180919073720_B14","doi-asserted-by":"publisher","first-page":"317","DOI":"10.1111\/j.1600-0714.2011.01006.x","article-title":"Helicobacter pylori in the oral cavity and gastric mucosa: a meta-analysis","volume":"40","author":"Zou","year":"2011","journal-title":"J. Oral Pathol. Med."},{"key":"key20180919073720_B15","doi-asserted-by":"publisher","first-page":"468","DOI":"10.1001\/archoto.2010.63","article-title":"Difference of Helicobacter pylori colonization in recurrent inflammatory and simple hyperplastic tonsil tissues","volume":"136","author":"Lin","year":"2010","journal-title":"Arch. Otolaryngol. Head Neck Surg."},{"key":"key20180919073720_B16","doi-asserted-by":"publisher","first-page":"807","DOI":"10.1016\/j.ijporl.2010.04.007","article-title":"Helicobacter pylori colonization of the adenotonsillar tissue: fact or fiction?","volume":"74","author":"Vilarinho","year":"2010","journal-title":"Int. J. Pediatr. Otorhinolaryngol."},{"key":"key20180919073720_B17","doi-asserted-by":"publisher","first-page":"406","DOI":"10.1007\/s00535-009-0169-9","article-title":"Coccoid Helicobacter pylori exists in the palatine tonsils of patients with IgA nephropathy","volume":"45","author":"Kusano","year":"2010","journal-title":"J. Gastroenterol."},{"key":"key20180919073720_B18","doi-asserted-by":"publisher","first-page":"133","DOI":"10.1038\/nrgastro.2011.1","article-title":"Vaccinating against Helicobacter pylori infection","volume":"8","author":"Czinn","year":"2011","journal-title":"Nat. Rev. Gastroenterol. Hepatol."},{"key":"key20180919073720_B19","doi-asserted-by":"publisher","first-page":"523","DOI":"10.1146\/annurev.immunol.19.1.523","article-title":"The design of vaccines against Helicobacter pylori and their development","volume":"19","author":"Del Giudice","year":"2001","journal-title":"Annu. Rev. Immunol."},{"key":"key20180919073720_B20","doi-asserted-by":"crossref","first-page":"5643","DOI":"10.1128\/jb.179.17.5643-5647.1997","article-title":"A flagellar sheath protein of Helicobacter pylori is identical to HpaA, a putative N-acetylneuraminyllactose-binding hemagglutinin, but is not an adhesin for AGS cells","volume":"179","author":"Jones","year":"1997","journal-title":"J. Bacteriol."},{"key":"key20180919073720_B21","doi-asserted-by":"crossref","first-page":"916","DOI":"10.1128\/IAI.65.3.916-923.1997","article-title":"Identification of the N-acetylneuraminyllactose-specific laminin-binding protein of Helicobacter pylori","volume":"65","author":"Valkonen","year":"1997","journal-title":"Infect. Immun."},{"key":"key20180919073720_B22","doi-asserted-by":"publisher","first-page":"82","DOI":"10.1046\/j.1523-5378.1999.98065.x","article-title":"Differences among Helicobacter pylori strains isolated from three different populations and demonstrated by restriction enzyme analysis of an internal fragment of the conserved gene hpaA","volume":"4","author":"Evans","year":"1999","journal-title":"Helicobacter"},{"key":"key20180919073720_B23","doi-asserted-by":"publisher","first-page":"176","DOI":"10.1038\/16495","article-title":"Genomic-sequence comparison of two unrelated isolates of the human gastric pathogen Helicobacter pylori","volume":"397","author":"Alm","year":"1999","journal-title":"Nature"},{"key":"key20180919073720_B24","doi-asserted-by":"crossref","first-page":"288","DOI":"10.1128\/CDLI.5.3.288-293.1998","article-title":"Specific antibodies in sera and gastric aspirates of symptomatic and asymptomatic Helicobacter pylori infected subjects","volume":"5","author":"Mattsson","year":"1998","journal-title":"Clin. Diagn. Lab. Immunol."},{"key":"key20180919073720_B25","first-page":"319","article-title":"Cloning, expression and bioinformatics analysis of Helicobacter pylori genes hp0231 and hp0410","volume":"7","author":"Hu","year":"2007","journal-title":"J. Trop. Med."},{"key":"key20180919073720_B26","doi-asserted-by":"publisher","first-page":"2591","DOI":"10.1016\/j.vaccine.2006.12.026","article-title":"Oral immunization with HpaA affords therapeutic protective immunity against H. pylori that is reflected by specific mucosal immune responses","volume":"25","author":"Nystr\u00f6m","year":"2007","journal-title":"Vaccine"},{"key":"key20180919073720_B27","doi-asserted-by":"crossref","first-page":"674","DOI":"10.1128\/jb.175.3.674-683.1993","article-title":"Cloning, nucleotide sequence, and expression of a gene encoding an adhesin subunit protein of Helicobacter pylori","volume":"175","author":"Evans","year":"1993","journal-title":"J. Bacteriol."},{"key":"key20180919073720_B28","doi-asserted-by":"publisher","first-page":"232","DOI":"10.1016\/S0958-1669(03)00033-8","article-title":"Metabolic pathway engineering in lactic acid bacteria","volume":"14","author":"Kleerebezem","year":"2003","journal-title":"Curr. Opin. Biotechnol."},{"key":"key20180919073720_B29","doi-asserted-by":"publisher","first-page":"222","DOI":"10.1016\/j.prevetmed.2007.02.008","article-title":"Safety and efficacy of an inactivated vaccine against Lactococcus garvieae in rainbow trout (Oncorhynchus mykiss)","volume":"80","author":"Vendrell","year":"2007","journal-title":"Prev. Vet. Med."},{"key":"key20180919073720_B30","doi-asserted-by":"publisher","first-page":"3655","DOI":"10.1016\/j.vaccine.2008.04.070","article-title":"Probiotic Lactobacillus acidophilus enhances the immunogenicity of an oral rotavirus vaccine in gnotobiotic pigs","volume":"26","author":"Zhang","year":"2008","journal-title":"Vaccine"},{"key":"key20180919073720_B31","doi-asserted-by":"publisher","first-page":"8049","DOI":"10.1016\/j.vaccine.2007.09.024","article-title":"Induction of mucosal and systemic immune responses following oral immunization of mice with Lactococcus lactis expressing human papillomavirus type 16 L1","volume":"25","author":"Cho","year":"2007","journal-title":"Vaccine"},{"key":"key20180919073720_B32","doi-asserted-by":"publisher","first-page":"2753","DOI":"10.1128\/IAI.72.5.2753-2761.2004","article-title":"Mucosal and cellular immune responses elicited by recombinant Lactococcus lactis strains expressing tetanus toxin fragment C","volume":"72","author":"Robinson","year":"2004","journal-title":"Infect. Immun."},{"key":"key20180919073720_B33","doi-asserted-by":"publisher","first-page":"1547","DOI":"10.1128\/IAI.69.3.1547-1553.2001","article-title":"Mucosal immune responses and protection against tetanus toxin after intranasal immunization with recombinant Lactobacillus plantarum","volume":"69","author":"Grangette","year":"2001","journal-title":"Infect. Immun."},{"key":"key20180919073720_B34","doi-asserted-by":"publisher","first-page":"1101","DOI":"10.1021\/bp0340077","article-title":"Fusion to a carrier protein and a synthetic propeptide enhances E7 HPV-16 production and secretion in Lactococcus lactis","volume":"19","author":"Berm\u00fadez-Humar\u00e1n","year":"2003","journal-title":"Biotechnol. Prog."},{"key":"key20180919073720_B35","doi-asserted-by":"publisher","first-page":"917","DOI":"10.1128\/AEM.68.2.917-922.2002","article-title":"Production of human papillomavirus type 16 E7 protein in Lactococcus lactis","volume":"68","author":"Berm\u00fadez-Humar\u00e1n","year":"2002","journal-title":"Appl. Environ. Microbiol."},{"key":"key20180919073720_B36","doi-asserted-by":"publisher","first-page":"223","DOI":"10.1182\/blood-2003-01-0110","article-title":"Immunogenicity and protective efficacy of orally administered recombinant Lactococcus lactis expressing surface-bound HIV Env","volume":"102","author":"Xin","year":"2003","journal-title":"Blood"},{"key":"key20180919073720_B37","doi-asserted-by":"publisher","first-page":"3927","DOI":"10.1016\/S0264-410X(01)00119-0","article-title":"Expression of Helicobacter pylori urease subunit B gene in Lactococcus lactis MG1363 and its use as a vaccine delivery system against H. pylori infection in mice","volume":"19","author":"Lee","year":"2001","journal-title":"Vaccine"},{"key":"key20180919073720_B38","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/S0065-2164(04)56001-X","article-title":"Potential and opportunities for use of recombinant lactic acid bacteria in human health","volume":"56","author":"Hanniffy","year":"2004","journal-title":"Adv. Appl. Microbiol."},{"key":"key20180919073720_B39","first-page":"129","article-title":"Electroporation of mycobacteria","volume":"101","author":"Parish","year":"1998","journal-title":"Methods Mol. Biol."},{"key":"key20180919073720_B40","doi-asserted-by":"publisher","first-page":"462","DOI":"10.1007\/s00253-005-0285-2","article-title":"Expression of Helicobacter pylori cag12 gene in Lactococcus lactis MG1363 and its oral administration to induce systemic anti-Cag12 immune response in mice","volume":"72","author":"Kim","year":"2006","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"key20180919073720_B41","doi-asserted-by":"crossref","first-page":"1386","DOI":"10.1016\/S0016-5085(97)70155-0","article-title":"A standardized mouse model of Helicobacter pylori infection: introducing the Sydney strain","volume":"112","author":"Lee","year":"1997","journal-title":"Gastroenterology"},{"key":"key20180919073720_B42","doi-asserted-by":"crossref","first-page":"1161","DOI":"10.1097\/00000478-199610000-00001","article-title":"Classification and grading of gastritis: the updated Sydney System: International Workshop on the Histopathology of Gastritis, Houston 1994","volume":"20","author":"Dixon","year":"1996","journal-title":"Am. J. Surg. Pathol."},{"key":"key20180919073720_B43","doi-asserted-by":"publisher","first-page":"2677","DOI":"10.1016\/S0264-410X(00)00052-9","article-title":"Further development of the Helicobacter pylori mouse vaccination model","volume":"18","author":"Sutton","year":"2000","journal-title":"Vaccine"},{"key":"key20180919073720_B44","doi-asserted-by":"publisher","first-page":"197","DOI":"10.1111\/j.1574-695X.2009.00566.x","article-title":"Oral vaccination of mice against Helicobacter pylori with recombinant Lactococcus lactis expressing urease subunit B","volume":"56","author":"Gu","year":"2009","journal-title":"FEMS Immunol. Med. Microbiol."},{"key":"key20180919073720_B45","doi-asserted-by":"publisher","first-page":"555","DOI":"10.1007\/s10096-010-1119-4","article-title":"Valuation of Helicobacter pylori eradication by triple therapy plus Lactobacillus acidophilus compared to triple therapy alone","volume":"30","author":"Medeiros","year":"2011","journal-title":"Eur. J. Clin. Microbiol. Infect. Dis."},{"key":"key20180919073720_B46","doi-asserted-by":"publisher","first-page":"499","DOI":"10.1007\/s11274-012-1203-2","article-title":"Inhibitory effect of Lactobacillus acidophilus on Helicobacter hepaticus in vitro","volume":"29","author":"Zhang","year":"2013","journal-title":"World J. Microbiol. Biotechnol."},{"key":"key20180919073720_B47","doi-asserted-by":"publisher","first-page":"466","DOI":"10.1111\/j.1523-5378.2012.00992.x","article-title":"Eradication of Helicobacter pylori infection by the probiotic strains Lactobacillus johnsonii MH-68 and L. salivarius ssp. salicinius AP-32","volume":"17","author":"Hsieh","year":"2012","journal-title":"Helicobacter"},{"key":"key20180919073720_B48","first-page":"59","article-title":"Evaluation of the antagonistic effect of Lactobacillus acidophilus on clinical strains of Helicobacter pylori","volume":"59","author":"Andrzejewska","year":"2007","journal-title":"Med. Dosw. Mikrobiol."},{"key":"key20180919073720_B49","doi-asserted-by":"publisher","first-page":"3655","DOI":"10.1016\/j.vaccine.2008.04.070","article-title":"Probiotic Lactobacillus acidophilus enhances the immunogenicity of an oral rotavirus vaccine in gnotobiotic pigs","volume":"26","author":"Zhang","year":"2008","journal-title":"Vaccine"},{"key":"key20180919073720_B50","doi-asserted-by":"publisher","first-page":"702","DOI":"10.1038\/nbt0702-702","article-title":"In situ delivery of passive immunity by lactobacilli producing single-chain antibodies","volume":"20","author":"Kr\u00fcger","year":"2002","journal-title":"Nat. Biotechnol."},{"key":"key20180919073720_B51","doi-asserted-by":"publisher","first-page":"2913","DOI":"10.1016\/S0264-410X(02)00229-3","article-title":"Recombinant Lactobacillus johnsonii as a mucosal vaccine delivery vehicle","volume":"20","author":"Scheppler","year":"2002","journal-title":"Vaccine"},{"key":"key20180919073720_B52","first-page":"265","article-title":"A study on main character of three lactic acid bacteria as vector host system","volume":"13","author":"Lu","year":"2001","journal-title":"Chin. J. Microecol."},{"key":"key20180919073720_B53","doi-asserted-by":"publisher","first-page":"920","DOI":"10.1128\/IAI.74.2.920-926.2006","article-title":"HpaA is essential for Helicobacter pylori colonization in mice","volume":"74","author":"Carlsohn","year":"2006","journal-title":"Infect. Immun."},{"key":"key20180919073720_B54","doi-asserted-by":"publisher","first-page":"6499","DOI":"10.1128\/IAI.70.11.6499-6503.2002","article-title":"Multiparameter selection of Helicobacter pylori antigens identifies two novel antigens with high protective efficacy","volume":"70","author":"Sabarth","year":"2002","journal-title":"Infect. Immun."}],"container-title":["Clinical and Vaccine Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1128\/CVI.00434-13","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,7,29]],"date-time":"2021-07-29T16:50:22Z","timestamp":1627577422000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/CVI.00434-13"}},"subtitle":[],"editor":[{"given":"D. L.","family":"Burns","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2013,11,27]]},"references-count":54,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2014,1,29]]},"published-print":{"date-parts":[[2014,2]]}},"alternative-id":["10.1128\/CVI.00434-13"],"URL":"https:\/\/doi.org\/10.1128\/cvi.00434-13","relation":{},"ISSN":["1556-6811","1556-679X"],"issn-type":[{"value":"1556-6811","type":"print"},{"value":"1556-679X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,11,27]]}}}