{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T14:33:10Z","timestamp":1761489190260},"reference-count":15,"publisher":"American Society for Microbiology","issue":"10","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clin. Vaccine Immunol."],"published-print":{"date-parts":[[2013,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Vaccination of cattle with a commercial human tuberculosis (TB) vaccine,<jats:named-content content-type=\"genus-species\">Mycobacterium bovis<\/jats:named-content>bacillus Calmette-Gu\u00e9rin (BCG) Danish, at a dose equivalent to 5 human doses of BCG has protected these animals against TB in field and experimental trials. There is interest in determining whether a 10-fold-lower dose could still protect cattle but not induce a tuberculin intradermal test response. Two groups of calves (<jats:italic>n<\/jats:italic>= 9\/group) were vaccinated subcutaneously with a lyophilized BCG Danish vaccine containing either 0.5 (1 \u00d7 10<jats:sup>5<\/jats:sup>to 4 \u00d7 10<jats:sup>5<\/jats:sup>CFU) or 5 (1 \u00d7 10<jats:sup>6<\/jats:sup>to 4 \u00d7 10<jats:sup>6<\/jats:sup>CFU) human doses of BCG Danish, with an additional group of 10 calves serving as nonvaccinated controls. Fifteen weeks after vaccination, these animals were challenged intratracheally with 5 \u00d7 10<jats:sup>3<\/jats:sup>CFU of virulent<jats:named-content content-type=\"genus-species\">M. bovis<\/jats:named-content>and another 15 weeks later were slaughtered and examined for the presence of tuberculous lesions. Vaccination of the calves with either 0.5 or 5 equivalent human doses of BCG Danish induced similar levels of protection against challenge with<jats:named-content content-type=\"genus-species\">M. bovis<\/jats:named-content>, with both groups showing significant reductions in the pathological and microbiological parameters compared to those for the the control group (<jats:italic>P<\/jats:italic>&lt; 0.05). Vaccination with either of the two BCG doses induced similar numbers of animals responding to the tuberculin intradermal test at 11 weeks postvaccination. Vaccination with a 0.5 equivalent human dose of a commercial lyophilized BCG vaccine can protect cattle against challenge with<jats:named-content content-type=\"genus-species\">M. bovis<\/jats:named-content>.<\/jats:p>","DOI":"10.1128\/cvi.00435-13","type":"journal-article","created":{"date-parts":[[2013,8,8]],"date-time":"2013-08-08T06:23:24Z","timestamp":1375943004000},"page":"1559-1562","source":"Crossref","is-referenced-by-count":24,"title":["Subcutaneous Administration of a 10-Fold-Lower Dose of a Commercial Human Tuberculosis Vaccine, Mycobacterium bovis Bacillus Calmette-Gu\u00e9rin Danish, Induced Levels of Protection against Bovine Tuberculosis and Responses in the Tuberculin Intradermal Test Similar to Those Induced by a Standard Cattle Dose"],"prefix":"10.1128","volume":"20","author":[{"given":"Bryce M.","family":"Buddle","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. 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