{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,9,23]],"date-time":"2023-09-23T22:32:15Z","timestamp":1695508335224},"reference-count":35,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2010,4,1]],"date-time":"2010-04-01T00:00:00Z","timestamp":1270080000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Vaccine Immunol"],"published-print":{"date-parts":[[2010,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            This study aimed to evaluate the use of protein A-peroxidase (horseradish peroxidase [HRPO]) in indirect enzyme-linked immunosorbent assays (iELISAs) and IgG avidity assays for serological distinction between\n            <jats:italic>Brucella abortus<\/jats:italic>\n            S19-vaccinated and -infected cows. Four groups were analyzed: GI, 41 nonvaccinated seropositive cows; GII, 79 S19-vaccinated heifers analyzed at 3 months postvaccination; GIII, 105 S19-vaccinated cows analyzed after 24 months of age; and GIV, 278 nonvaccinated seronegative cows. IgG levels and avidity to\n            <jats:italic>B. abortus<\/jats:italic>\n            smooth lipopolysaccharide (S-LPS) were determined using anti-bovine IgG-HRPO or protein A-HRPO conjugates. Similar levels of IgG anti-S-LPS were found with GI using both conjugates. Lower IgG levels were detected with GII, GIII, and GIV using protein A-HRPO. Both conjugates showed high performance in discriminating GI from GIII, with high sensitivity (Se; 97.6%) and specificity (Sp; 97.1%). Protein A-HRPO was better in distinguishing GI from GIV (Se, 97.6%; Sp, 94.6%) and GI from GII (Se, 80.5%; Sp, 94.9%). Protein A-HRPO excluded a higher number of positive samples with GII and GIV. IgG avidity showed that protein A-HRPO, but not anti-IgG-HRPO, was able to distinguish nonvaccinated from vaccinated cattle, showing a higher avidity index (AI) with GI than with GII, with 78% of serum samples in GII showing an AI of &lt;50%. Therefore, the iELISA using\n            <jats:italic>B. abortus<\/jats:italic>\n            S-LPS antigen and protein A-HRPO conjugate for preferential detection of the IgG2 subclass was shown to be suitable for serological distinction between S19-vaccinated and -infected cows. Also, antibodies generated after vaccination showed lower avidity, suggesting a role for the IgG2 subclass as an antibody of higher-affinity maturation after infection, constituting an additional tool for differentiating vaccinated from infected cattle.\n          <\/jats:p>","DOI":"10.1128\/cvi.00444-09","type":"journal-article","created":{"date-parts":[[2010,2,11]],"date-time":"2010-02-11T04:45:07Z","timestamp":1265863507000},"page":"588-595","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":7,"title":["Evaluation of Indirect Enzyme-Linked Immunosorbent Assays and IgG Avidity Assays Using a Protein A-Peroxidase Conjugate for Serological Distinction between\n            <i>Brucella abortus<\/i>\n            S19-Vaccinated and -Infected Cows"],"prefix":"10.1128","volume":"17","author":[{"given":"Ana C. A. M.","family":"Pajuaba","sequence":"first","affiliation":[{"name":"Laboratory of Immunoparasitology, Institute of Biomedical Sciences, Federal University of Uberla\u0302ndia, 38400-902 Uberla\u0302ndia, MG, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Deise A. O.","family":"Silva","sequence":"additional","affiliation":[{"name":"Laboratory of Immunoparasitology, Institute of Biomedical Sciences, Federal University of Uberla\u0302ndia, 38400-902 Uberla\u0302ndia, MG, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jose\u0301 R.","family":"Mineo","sequence":"additional","affiliation":[{"name":"Laboratory of Immunoparasitology, Institute of Biomedical Sciences, Federal University of Uberla\u0302ndia, 38400-902 Uberla\u0302ndia, MG, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"161","volume":"7","year":"2000","unstructured":"Abalos, P., J. Daffner, and L. 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