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The preparation consisted mostly of polysaccharide (PS; &gt;90% [wt\/wt]; both cell-associated PS and exo-PS were combined) and a small amount of protein (1 to 3%) with no apparent nucleic acids. Vaccinated mice were protected (these had a statistically significant reduction in bacterial colonization compared to that of unvaccinated controls) when challenged with representative strains of three<jats:named-content content-type=\"genus-species\">Brucella<\/jats:named-content>species most pathogenic for humans, i.e.,<jats:named-content content-type=\"genus-species\">B. abortus<\/jats:named-content>,<jats:named-content content-type=\"genus-species\">B. melitensis<\/jats:named-content>, and<jats:named-content content-type=\"genus-species\">B. suis<\/jats:named-content>. As little as 1 ng of the vaccine, without added adjuvant, protected mice against<jats:named-content content-type=\"genus-species\">B. suis<\/jats:named-content>145 infection (5 \u00d7 10<jats:sup>5<\/jats:sup>CFU), and a single injection of 1 \u03bcg of this subunit vaccine protected mice from<jats:named-content content-type=\"genus-species\">B. suis<\/jats:named-content>145 challenge for at least 14 months. A single immunization induced a serum IgG response to<jats:named-content content-type=\"genus-species\">Brucella<\/jats:named-content>antigens that remained elevated for up to 9 weeks. The use of heat (i.e., boiling-water bath, autoclaving) in the vaccine preparation showed that it was thermostable. This method also ensured safety and security. The vaccine produced was immunogenic and highly protective against multiple strains of<jats:named-content content-type=\"genus-species\">Brucella<\/jats:named-content>and represents a promising candidate for further evaluation.<\/jats:p>","DOI":"10.1128\/cvi.00447-14","type":"journal-article","created":{"date-parts":[[2014,10,16]],"date-time":"2014-10-16T05:58:52Z","timestamp":1413439132000},"page":"1681-1688","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":6,"title":["Thermostable Cross-Protective Subunit Vaccine against Brucella Species"],"prefix":"10.1128","volume":"21","author":[{"given":"John W.","family":"Cherwonogrodzky","sequence":"first","affiliation":[{"name":"Defence Research and Development Canada (DRDC), Suffield Research Centre, Medicine Hat, Alberta, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicole D.","family":"Barab\u00e9","sequence":"additional","affiliation":[{"name":"Defence Research and Development Canada (DRDC), Suffield Research Centre, Medicine Hat, Alberta, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michelle L.","family":"Grigat","sequence":"additional","affiliation":[{"name":"Defence Research and Development Canada (DRDC), Suffield Research Centre, Medicine Hat, Alberta, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"William E.","family":"Lee","sequence":"additional","affiliation":[{"name":"Defence Research and Development Canada (DRDC), Suffield Research Centre, Medicine Hat, Alberta, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert T.","family":"Poirier","sequence":"additional","affiliation":[{"name":"Defence Research and Development Canada (DRDC), Suffield Research Centre, Medicine Hat, Alberta, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Scott J.","family":"Jager","sequence":"additional","affiliation":[{"name":"Defence Research and Development Canada (DRDC), Suffield Research Centre, Medicine Hat, Alberta, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bradley J.","family":"Berger","sequence":"additional","affiliation":[{"name":"Defence Research and Development Canada (DRDC), Suffield Research Centre, Medicine Hat, Alberta, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(06)70382-6"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.20506\/rst.25.1.1646"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-006-6311-4"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.2174\/1574891X11308010006"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0002800"},{"key":"e_1_3_3_7_2","first-page":"21","article-title":"Brucellosis in humans: its diagnosis and treatment","volume":"3","author":"Williams E","year":"1988","unstructured":"WilliamsE. 1988. 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