{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T18:19:57Z","timestamp":1773339597804,"version":"3.50.1"},"reference-count":36,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2010,5,1]],"date-time":"2010-05-01T00:00:00Z","timestamp":1272672000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Vaccine Immunol"],"published-print":{"date-parts":[[2010,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The\n            <jats:italic>Bordetella pertussis<\/jats:italic>\n            pertussis toxin and pertactin (Prn) are protective antigens and are contained in acellular pertussis vaccines. Polymorphisms in the A subunit of pertussis toxin (PtxA) and pertactin have been proposed to mediate vaccine resistance and contribute to pertussis reemergence. To test this hypothesis, previous studies compared clinical isolates expressing different alleles for the proteins. However, other virulence factors or virulence factor expression levels also may vary, confounding the analysis. To overcome these limitations, we constructed isogenic mutants of\n            <jats:italic>B. pertussis<\/jats:italic>\n            Tohama expressing the alleles\n            <jats:italic>ptxA1<\/jats:italic>\n            or\n            <jats:italic>ptxA2<\/jats:italic>\n            and\n            <jats:italic>prn1<\/jats:italic>\n            or\n            <jats:italic>prn2<\/jats:italic>\n            and compared the efficacies of an acellular pertussis vaccine against the mutants in a mouse model. While the vaccine was effective against all of the\n            <jats:italic>B. pertussis<\/jats:italic>\n            strains regardless of the allele expression pattern, the strain expressing\n            <jats:italic>ptxA1<\/jats:italic>\n            and\n            <jats:italic>prn2<\/jats:italic>\n            displayed a survival advantage over the other strains. These results suggest that an allele shift to the\n            <jats:italic>ptxA1 prn2<\/jats:italic>\n            genotype may play a role in the emergence of pertussis in vaccinated populations.\n          <\/jats:p>","DOI":"10.1128\/cvi.00449-09","type":"journal-article","created":{"date-parts":[[2010,4,1]],"date-time":"2010-04-01T02:43:51Z","timestamp":1270089831000},"page":"807-812","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":28,"title":["Synergic Effect of Genotype Changes in Pertussis Toxin and Pertactin on Adaptation to an Acellular Pertussis Vaccine in the Murine Intranasal Challenge Model"],"prefix":"10.1128","volume":"17","author":[{"given":"Eiji","family":"Komatsu","sequence":"first","affiliation":[{"name":"Kitasato Institute, Research Center for Biologicals, 6-111 Arai, Kitamoto, Saitama 364-0026, Japan"},{"name":"Kitasato University, Graduate School of Infection Control Sciences, 5-9-1 Shirokane, Minato-ku, Tokyo 108-8641, Japan"},{"name":"Department of Molecular Genetics, Biochemistry, and Microbiology, University of Cincinnati, 231 Albert Sabin Way, Cincinnati, Ohio 45267-0524"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fuminori","family":"Yamaguchi","sequence":"additional","affiliation":[{"name":"Kitasato Institute, Research Center for Biologicals, 6-111 Arai, Kitamoto, Saitama 364-0026, Japan"},{"name":"Kitasato University, Graduate School of Infection Control Sciences, 5-9-1 Shirokane, Minato-ku, Tokyo 108-8641, Japan"},{"name":"Department of Molecular Genetics, Biochemistry, and Microbiology, University of Cincinnati, 231 Albert Sabin Way, Cincinnati, Ohio 45267-0524"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akio","family":"Abe","sequence":"additional","affiliation":[{"name":"Kitasato Institute, Research Center for Biologicals, 6-111 Arai, Kitamoto, Saitama 364-0026, Japan"},{"name":"Kitasato University, Graduate School of Infection Control Sciences, 5-9-1 Shirokane, Minato-ku, Tokyo 108-8641, Japan"},{"name":"Department of Molecular Genetics, Biochemistry, and Microbiology, University of Cincinnati, 231 Albert Sabin Way, Cincinnati, Ohio 45267-0524"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alison A.","family":"Weiss","sequence":"additional","affiliation":[{"name":"Kitasato Institute, Research Center for Biologicals, 6-111 Arai, Kitamoto, Saitama 364-0026, Japan"},{"name":"Kitasato University, Graduate School of Infection Control Sciences, 5-9-1 Shirokane, Minato-ku, Tokyo 108-8641, Japan"},{"name":"Department of Molecular Genetics, Biochemistry, and Microbiology, University of Cincinnati, 231 Albert Sabin Way, Cincinnati, Ohio 45267-0524"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mineo","family":"Watanabe","sequence":"additional","affiliation":[{"name":"Kitasato Institute, Research Center for Biologicals, 6-111 Arai, Kitamoto, Saitama 364-0026, Japan"},{"name":"Kitasato University, Graduate School of Infection Control Sciences, 5-9-1 Shirokane, Minato-ku, Tokyo 108-8641, Japan"},{"name":"Department of Molecular Genetics, Biochemistry, and Microbiology, University of Cincinnati, 231 Albert Sabin Way, Cincinnati, Ohio 45267-0524"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00177-07"},{"key":"e_1_3_2_3_2","doi-asserted-by":"crossref","first-page":"2651","DOI":"10.1016\/S0264-410X(99)00038-9","volume":"17","year":"1999","unstructured":"Boursaux-Eude, C., S. 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