{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,28]],"date-time":"2025-09-28T11:04:14Z","timestamp":1759057454283,"version":"3.40.5"},"reference-count":49,"publisher":"American Society for Microbiology","issue":"11","license":[{"start":{"date-parts":[[2015,11,1]],"date-time":"2015-11-01T00:00:00Z","timestamp":1446336000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Vaccine Immunol"],"published-print":{"date-parts":[[2015,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Current vaccinations are effective against encapsulated strains of\n            <jats:named-content content-type=\"genus-species\">Streptococcus pneumoniae<\/jats:named-content>\n            , but they do not protect against nonencapsulated\n            <jats:named-content content-type=\"genus-species\">Streptococcus pneumoniae<\/jats:named-content>\n            (NESp), which is increasing in colonization and incidence of pneumococcal disease. Vaccination with pneumococcal proteins has been assessed for its ability to protect against pneumococcal disease, but several of these proteins are not expressed by NESp. Pneumococcal surface protein K (PspK), an NESp virulence factor, has not been assessed for immunogenic potential or host modulatory effects. Mammalian cytokine expression was determined in an\n            <jats:italic>in vivo<\/jats:italic>\n            mouse model and in an\n            <jats:italic>in vitro<\/jats:italic>\n            cell culture system. Systemic and mucosal mouse immunization studies were performed to determine the immunogenic potential of PspK. Murine serum and saliva were collected to quantitate specific antibody isotype responses and the ability of antibody and various proteins to inhibit epithelial cell adhesion. Host cytokine response was not reduced by PspK. NESp was able to colonize the mouse nasopharynx as effectively as encapsulated pneumococci. Systemic and mucosal immunization provided protection from colonization by PspK-positive (PspK\n            <jats:sup>+<\/jats:sup>\n            ) NESp. Anti-PspK antibodies were recovered from immunized mice and significantly reduced the ability of NESp to adhere to human epithelial cells. A protein-based pneumococcal vaccine is needed to provide broad protection against encapsulated and nonencapsulated pneumococci in an era of increasing antibiotic resistance and vaccine escape mutants. We demonstrate that PspK may serve as an NESp target for next-generation pneumococcal vaccines. Immunization with PspK protected against pneumococcal colonization, which is requisite for pneumococcal disease.\n          <\/jats:p>","DOI":"10.1128\/cvi.00456-15","type":"journal-article","created":{"date-parts":[[2015,8,27]],"date-time":"2015-08-27T02:09:14Z","timestamp":1440641354000},"page":"1146-1153","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":23,"title":["Immunization with Pneumococcal Surface Protein K of Nonencapsulated Streptococcus pneumoniae Provides Protection in a Mouse Model of Colonization"],"prefix":"10.1128","volume":"22","author":[{"given":"Lance E.","family":"Keller","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, University of Mississippi Medical Center, Jackson, Mississippi, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiao","family":"Luo","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Mississippi Medical Center, Jackson, Mississippi, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Justin A.","family":"Thornton","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Mississippi State University, Mississippi State, Mississippi, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9280-9680","authenticated-orcid":false,"given":"Keun-Seok","family":"Seo","sequence":"additional","affiliation":[{"name":"Department of Basic Sciences, College of Veterinary Medicine, Mississippi State University, Mississippi State, Mississippi, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bo Youn","family":"Moon","sequence":"additional","affiliation":[{"name":"Department of Basic Sciences, College of Veterinary Medicine, Mississippi State University, Mississippi State, Mississippi, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D. 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