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Anticapsular antibodies (Abs) were developed because disseminated infections caused by hv\n            <jats:italic>Kp<\/jats:italic>\n            are associated with significant morbidity and mortality, even with antibiotic-sensitive strains. K1-serotype polysaccharide capsules (K1-CPS) are expressed by the majority of hv\n            <jats:italic>Kp<\/jats:italic>\n            strains. In this study, K1-CPS-specific IgG Abs were generated by conjugation of K1-CPS to immunogenic anthrax protective antigen (PA) protein. Opsonophagocytic efficacy was measured\n            <jats:italic>in vitro<\/jats:italic>\n            and\n            <jats:italic>in vivo<\/jats:italic>\n            by intravital microscopy in murine livers.\n            <jats:italic>In vivo<\/jats:italic>\n            protection was tested in murine models, including a novel model for dissemination in hv\n            <jats:italic>Kp<\/jats:italic>\n            -colonized mice. Protective efficacy of monoclonal antibodies (MAbs) 4C5 (IgG1) and 19A10 (IgG3) was demonstrated both in murine sepsis and pulmonary infection. In hv\n            <jats:italic>Kp<\/jats:italic>\n            -colonized mice, MAb treatment significantly decreased dissemination of hv\n            <jats:italic>Kp<\/jats:italic>\n            from the gut to mesenteric lymph nodes and organs. Intravital microscopy confirmed efficient opsonophagocytosis and clearance of bacteria from the liver.\n            <jats:italic>In vitro<\/jats:italic>\n            studies demonstrate that MAbs work predominantly by promoting FcR-mediated phagocytosis but also indicate that MAbs enhance the release of neutrophil extracellular traps (NETs). In anticipation of increasing antibiotic resistance, we propose further development of these and other\n            <jats:named-content content-type=\"genus-species\">Klebsiella<\/jats:named-content>\n            -specific MAbs for therapeutic use.\n          <\/jats:p>","DOI":"10.1128\/cvi.00456-16","type":"journal-article","created":{"date-parts":[[2016,10,27]],"date-time":"2016-10-27T03:00:19Z","timestamp":1477537219000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":86,"title":["Antibody-Based Immunotherapy To Treat and Prevent Infection with Hypervirulent Klebsiella pneumoniae"],"prefix":"10.1128","volume":"24","author":[{"given":"Elizabeth","family":"Diago-Navarro","sequence":"first","affiliation":[{"name":"Department of Medicine (Division of Infectious Diseases), Stony Brook University, Stony Brook, New York, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabel","family":"Calatayud-Baselga","sequence":"additional","affiliation":[{"name":"Universidad Francisco de Vitoria, Pozuelo de Alarc\u00f3n, Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Donglei","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, University of Maryland at College Park, College Park, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Camille","family":"Khairallah","sequence":"additional","affiliation":[{"name":"Department of Molecular Genetics and Microbiology, Stony Brook University, Stony Brook, New York, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Inderjit","family":"Mann","sequence":"additional","affiliation":[{"name":"Department of Medicine (Division of Infectious Diseases), Stony Brook University, Stony Brook, New York, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amaia","family":"Ulacia-Hernando","sequence":"additional","affiliation":[{"name":"Universidad Francisco de Vitoria, Pozuelo de Alarc\u00f3n, Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brian","family":"Sheridan","sequence":"additional","affiliation":[{"name":"Department of Molecular Genetics and Microbiology, Stony Brook University, Stony Brook, New York, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meiqing","family":"Shi","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, University of Maryland at College Park, College Park, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bettina C.","family":"Fries","sequence":"additional","affiliation":[{"name":"Department of Medicine (Division of Infectious Diseases), Stony Brook University, Stony Brook, New York, USA"},{"name":"Department of Molecular Genetics and Microbiology, Stony Brook University, Stony Brook, New York, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.01355-14"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01775-15"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.4161\/viru.22718"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.2174\/1874285801105010107"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.46964-0"},{"key":"e_1_3_3_7_2","first-page":"232","article-title":"Comparison of pyogenic liver abscesses caused by Klebsiella pneumoniae and non-K. pneumoniae pathogens","volume":"94","author":"Chang FY","year":"1995","unstructured":"Chang FY ChouMY. 1995. 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