{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,18]],"date-time":"2026-05-18T11:25:34Z","timestamp":1779103534336,"version":"3.51.4"},"reference-count":26,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2008,3,1]],"date-time":"2008-03-01T00:00:00Z","timestamp":1204329600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Vaccine Immunol"],"published-print":{"date-parts":[[2008,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>The 53-kDa proteins in larval excretory and secretory (E-S) products were expressed from five<jats:italic>Trichinella<\/jats:italic>species (<jats:italic>T. spiralis<\/jats:italic>,<jats:italic>T. britovi<\/jats:italic>,<jats:italic>T. nativa<\/jats:italic>,<jats:italic>T. pseudospiralis<\/jats:italic>, and<jats:italic>T. papuae<\/jats:italic>), using the<jats:italic>Escherichia coli<\/jats:italic>expression system, and the antibody responses to the 53-kDa recombinant proteins in mice infected with<jats:italic>Trichinella<\/jats:italic>spp. were analyzed by Western blotting. The 53-kDa protein is conserved among the five<jats:italic>Trichinella<\/jats:italic>species, with &gt;60% similarity in amino acid sequences. The 53-kDa recombinant proteins of<jats:italic>T. spiralis<\/jats:italic>and<jats:italic>T. pseudospiralis<\/jats:italic>reacted to sera from mice infected with<jats:italic>T. spiralis<\/jats:italic>and<jats:italic>T. pseudospiralis<\/jats:italic>at 8 days postinfection (p.i.), respectively. An antibody against the 53-kDa recombinant protein of<jats:italic>T. spiralis<\/jats:italic>recognized the 53-kDa protein in the crude extracts from adult worms and 30-day p.i. muscle larvae and E-S products from muscle larvae of<jats:italic>T. spiralis<\/jats:italic>but did not recognize any proteins from<jats:italic>T. pseudospiralis<\/jats:italic>. The sera from the mice infected with<jats:italic>T. spiralis<\/jats:italic>strongly reacted with the 53-kDa recombinant protein of<jats:italic>T. spiralis<\/jats:italic>but did not react with the 53-kDa recombinant proteins of<jats:italic>T. britovi<\/jats:italic>,<jats:italic>T. nativa<\/jats:italic>,<jats:italic>T. pseudospiralis<\/jats:italic>, and<jats:italic>T. papuae<\/jats:italic>. Similarly, the sera from mice infected with<jats:italic>T. britovi<\/jats:italic>,<jats:italic>T. nativa<\/jats:italic>,<jats:italic>T. pseudospiralis<\/jats:italic>, or<jats:italic>T. papuae<\/jats:italic>strongly reacted with the 53-kDa recombinant proteins of<jats:italic>T. britovi<\/jats:italic>,<jats:italic>T. nativa<\/jats:italic>,<jats:italic>T. pseudospiralis<\/jats:italic>, or<jats:italic>T. papuae<\/jats:italic>, respectively. These results showed that the 53-kDa recombinant proteins provide early and species-specific antibody responses in mice infected with<jats:italic>Trichinella<\/jats:italic>spp.<\/jats:p>","DOI":"10.1128\/cvi.00467-07","type":"journal-article","created":{"date-parts":[[2008,1,10]],"date-time":"2008-01-10T02:05:54Z","timestamp":1199930754000},"page":"468-473","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":24,"title":["Species-Specific Antibody Responses to the Recombinant 53-Kilodalton Excretory and Secretory Proteins in Mice Infected with<i>Trichinella<\/i>spp"],"prefix":"10.1128","volume":"15","author":[{"given":"Isao","family":"Nagano","sequence":"first","affiliation":[{"name":"Department of Parasitology, Gifu University Graduate School of Medicine, Yanagido 1-1, Gifu, 501-1194 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhiliang","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Gifu University Graduate School of Medicine, Yanagido 1-1, Gifu, 501-1194 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuzo","family":"Takahashi","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Gifu University Graduate School of Medicine, Yanagido 1-1, Gifu, 501-1194 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_1_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.9.1.47"},{"key":"e_1_3_1_3_2","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1016\/S0378-4347(98)00094-2","volume":"714","year":"1998","unstructured":"Colangeli, R., A. 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