{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T21:09:55Z","timestamp":1791407395277,"version":"4.3.3"},"reference-count":37,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2012,12,1]],"date-time":"2012-12-01T00:00:00Z","timestamp":1354320000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Vaccine Immunol"],"published-print":{"date-parts":[[2012,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            We have developed a novel vaccine against Shiga toxin (Stx)-producing\n            <jats:named-content content-type=\"genus-species\">Escherichia coli<\/jats:named-content>\n            (STEC) infection using a recombinant\n            <jats:named-content content-type=\"genus-species\">Mycobacterium bovis<\/jats:named-content>\n            BCG (rBCG) system. Two intraperitoneal vaccinations with rBCG expressing the Stx2 B subunit (Stx2B) resulted in an increase of protective serum IgG and mucosal IgA responses to Stx2B in BALB\/c mice. When orally challenged with 10\n            <jats:sup>3<\/jats:sup>\n            CFU of STEC strain B2F1 (O91: H21), the immunized mice survived statistically significantly longer than the nonvaccinated mice. We suggest that intraperitoneal immunization with rBCG expressing Stx2B would be a potential vaccine strategy for STEC.\n          <\/jats:p>","DOI":"10.1128\/cvi.00473-12","type":"journal-article","created":{"date-parts":[[2012,10,4]],"date-time":"2012-10-04T03:04:16Z","timestamp":1349319856000},"page":"1932-1937","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":18,"title":["Protection by a Recombinant Mycobacterium bovis Bacillus Calmette-Gu\u00e9rin Vaccine Expressing Shiga Toxin 2 B Subunit against Shiga Toxin-Producing Escherichia coli in Mice"],"prefix":"10.1128","volume":"19","author":[{"given":"Jun","family":"Fujii","sequence":"first","affiliation":[{"name":"Department of Bacteriology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariko","family":"Naito","sequence":"additional","affiliation":[{"name":"Microbiology and Oral Infection, Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takashi","family":"Yutsudo","sequence":"additional","affiliation":[{"name":"Discovery Research Laboratory, Shionogi &amp; Co., Ltd., Toyonaka City, Osaka, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sohkichi","family":"Matsumoto","sequence":"additional","affiliation":[{"name":"Department of Bacteriology, Osaka City University Graduate School of Medicine, Osaka, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel P.","family":"Heatherly","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine\/Nephrology, University of Virginia, Charlottesville, Virginia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takeshi","family":"Yamada","sequence":"additional","affiliation":[{"name":"Microbiology and Oral Infection, Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hideyuki","family":"Kobayashi","sequence":"additional","affiliation":[{"name":"Department of Chemistry, School of Medicine, University of Occupational and Environmental Health, Kitakyushu City, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shin-ichi","family":"Yoshida","sequence":"additional","affiliation":[{"name":"Department of Bacteriology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tom","family":"Obrig","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.64.1.355-357.1996"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.64.1.55-60.1996"},{"key":"e_1_3_3_4_2","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1093\/intimm\/2.1.1","article-title":"Expression of glycolipid receptors to Shiga-like toxin on human B lymphocytes: a mechanism for the failure of long-lived antibody response to dysenteric disease","volume":"2","author":"Cohen A","year":"1990","unstructured":"CohenA. 1990. 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