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We evaluated the diagnostic potential of specific T-cell epitopes selected from two immunodominant antigens, Rv1985c and Rv3425, from RD2 and RD11, respectively, on the basis of epitope mapping, in TB patients and BCG-vaccinated healthy individuals. Using a whole-blood gamma interferon release assay, a wide array of epitopes was recognized on both Rv1985c and Rv3425 in TB patients. Those epitopes that could specifically discriminate TB infection from BCG vaccination were carefully selected, and the most promising peptide pools from Rv1985c showed a sensitivity of 53.9% and a specificity of 95.5%. When the novel specific peptides from Rv1985c joined the diagnostic antigens in the QuantiFERON-TB Gold In-Tube (QFT-IT) assay, the sensitivity was increased from 86.4% to 96.2%, with no drop in specificity. These results indicate that the peptide pools selected from Rv1985c and Rv3425 have the potential to diagnose TB infection by a method that may be routinely used in clinical laboratories.<\/jats:p>","DOI":"10.1128\/cvi.00481-12","type":"journal-article","created":{"date-parts":[[2012,11,8]],"date-time":"2012-11-08T07:26:35Z","timestamp":1352359595000},"page":"69-76","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":14,"title":["Mycobacterium tuberculosis Region of Difference (RD) 2 Antigen Rv1985c and RD11 Antigen Rv3425 Have the Promising Potential To Distinguish Patients with Active Tuberculosis from M. bovis BCG-Vaccinated Individuals"],"prefix":"10.1128","volume":"20","author":[{"given":"Sen","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Infectious Diseases, Huashan Hospital, Fudan University, Shanghai, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiazhen","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Huashan Hospital, Fudan University, Shanghai, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ying","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Huashan Hospital, Fudan University, Shanghai, China"},{"name":"Department of Molecular Microbiology and Immunology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, Maryland, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ni","family":"Diao","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Huashan Hospital, Fudan University, Shanghai, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shu","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Huashan Hospital, Fudan University, Shanghai, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Huashan Hospital, Fudan University, Shanghai, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chanyi","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Huashan Hospital, Fudan University, Shanghai, China"},{"name":"State Key Laboratory of Genetic Engineering, Institute of Genetics, Fudan University, Shanghai, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Feifei","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Huashan Hospital, Fudan University, Shanghai, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan","family":"Gao","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Huashan Hospital, Fudan University, Shanghai, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lingyun","family":"Shao","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Huashan Hospital, Fudan University, Shanghai, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jialin","family":"Jin","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Huashan Hospital, Fudan University, Shanghai, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xinhua","family":"Weng","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Huashan Hospital, Fudan University, Shanghai, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenhong","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Huashan Hospital, Fudan University, Shanghai, China"},{"name":"Institute of Biomedical Science, Fudan University, Shanghai, China"},{"name":"Ministry of Health and Ministry of Education Key Laboratory of Medical Molecular Virology, Shanghai Medical College, Fudan University, Shanghai, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"532","DOI":"10.1055\/s-0028-1085704","article-title":"New approaches to the treatment of latent tuberculosis","volume":"29","author":"Sterling TR","year":"2008","unstructured":"SterlingTR. 2008. 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