{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T06:50:51Z","timestamp":1787899851203,"version":"build-2784847793"},"reference-count":31,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2009,5,1]],"date-time":"2009-05-01T00:00:00Z","timestamp":1241136000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Vaccine Immunol"],"published-print":{"date-parts":[[2009,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Differentiation of latent tuberculosis infection (LTBI) from a healthy, unexposed population plays a vital role in the strategy of controlling and eliminating tuberculosis (TB). Both CFP21 and MPT64, antigens encoded by the RD2 region which are restricted in the\n            <jats:italic>Mycobacterium tuberculosis<\/jats:italic>\n            complex, are TB-specific diagnostic candidate antigens. In this study, we designed a fusion protein by linking both CFP21 and MPT64 with a 15-amino-acid peptide, (G\n            <jats:sub>4<\/jats:sub>\n            S\n            <jats:sub>1<\/jats:sub>\n            )\n            <jats:sub>3<\/jats:sub>\n            , and overexpressed the fusion protein in\n            <jats:italic>Escherichia coli<\/jats:italic>\n            . A new whole-blood gamma interferon assay based on the recombinant fusion protein, CFP21-MPT64 (rCM-WBIA), was developed and compared with the tuberculin skin test (TST) for screening of LTBI in household contacts of patients with sputum-positive TB. rCM-WBIA had a slightly higher sensitivity (66.7%; 24\/36 contacts) than that of the TST (61.1%; 22\/36 contacts) for household contacts. We found that rCM-WBIA had a very high sensitivity (90.9%) and specificity (71.4%) for LTBI detection compared with TST. The overall agreement between rCM-WBIA and TST was 83.3% (\n            <jats:italic>k<\/jats:italic>\n            = 0.64); rCM-WBIA positivity was associated with a larger TST induration. These results suggest that rCM-WBIA, based on the recombinant fusion protein CFP21-MPT64, is a promising alternative diagnostic tool for detection of LTBI.\n          <\/jats:p>","DOI":"10.1128\/cvi.00486-08","type":"journal-article","created":{"date-parts":[[2009,3,12]],"date-time":"2009-03-12T01:58:17Z","timestamp":1236823097000},"page":"686-691","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":29,"title":["An Improved Whole-Blood Gamma Interferon Assay Based on the CFP21-MPT64 Fusion Protein"],"prefix":"10.1128","volume":"16","author":[{"given":"Ruiling","family":"Fu","sequence":"first","affiliation":[{"name":"Laboratory of Bio-Safety, Department of Pathogen Biology, Tongji Medical College, Huazhong University of Science &amp; Technology, Wuhan 430030, People's Republic of China"},{"name":"Institute of Virology, University Hospital Essen, University Duisburg-Essen, Essen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chun","family":"Wang","sequence":"additional","affiliation":[{"name":"Laboratory of Bio-Safety, Department of Pathogen Biology, Tongji Medical College, Huazhong University of Science &amp; Technology, Wuhan 430030, People's Republic of China"},{"name":"Institute of Virology, University Hospital Essen, University Duisburg-Essen, Essen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunwei","family":"Shi","sequence":"additional","affiliation":[{"name":"Laboratory of Bio-Safety, Department of Pathogen Biology, Tongji Medical College, Huazhong University of Science &amp; Technology, Wuhan 430030, People's Republic of China"},{"name":"Institute of Virology, University Hospital Essen, University Duisburg-Essen, Essen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengji","family":"Lu","sequence":"additional","affiliation":[{"name":"Laboratory of Bio-Safety, Department of Pathogen Biology, Tongji Medical College, Huazhong University of Science &amp; Technology, Wuhan 430030, People's Republic of China"},{"name":"Institute of Virology, University Hospital Essen, University Duisburg-Essen, Essen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhengming","family":"Fang","sequence":"additional","affiliation":[{"name":"Laboratory of Bio-Safety, Department of Pathogen Biology, Tongji Medical College, Huazhong University of Science &amp; Technology, Wuhan 430030, People's Republic of China"},{"name":"Institute of Virology, University Hospital Essen, University Duisburg-Essen, Essen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jia","family":"Lu","sequence":"additional","affiliation":[{"name":"Laboratory of Bio-Safety, Department of Pathogen Biology, Tongji Medical College, Huazhong University of Science &amp; Technology, Wuhan 430030, People's Republic of China"},{"name":"Institute of Virology, University Hospital Essen, University Duisburg-Essen, Essen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fang","family":"Wang","sequence":"additional","affiliation":[{"name":"Laboratory of Bio-Safety, Department of Pathogen Biology, Tongji Medical College, Huazhong University of Science &amp; Technology, Wuhan 430030, People's Republic of China"},{"name":"Institute of Virology, University Hospital Essen, University Duisburg-Essen, Essen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xionglin","family":"Fan","sequence":"additional","affiliation":[{"name":"Laboratory of Bio-Safety, Department of Pathogen Biology, Tongji Medical College, Huazhong University of Science &amp; Technology, Wuhan 430030, People's Republic of China"},{"name":"Institute of Virology, University Hospital Essen, University Duisburg-Essen, Essen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"1099","DOI":"10.1016\/S0140-6736(00)02742-2","volume":"356","year":"2000","unstructured":"Andersen, P., M. 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