{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T04:43:50Z","timestamp":1789879430857,"version":"4.0.1"},"reference-count":29,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2016,3,1]],"date-time":"2016-03-01T00:00:00Z","timestamp":1456790400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100006022","name":"Minist\u00e8re des Affaires Sociales, de la Sant\u00e9 et des Droits des Femmes","doi-asserted-by":"publisher","award":["N\/2009N56 SOPHIA"],"award-info":[{"award-number":["N\/2009N56 SOPHIA"]}],"id":[{"id":"10.13039\/501100006022","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Vaccine Immunol"],"published-print":{"date-parts":[[2016,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Allergic bronchopulmonary aspergillosis (ABPA) is difficult to diagnose; diagnosis relies on clinical, radiological, pathological, and serological criteria. Our aim was to assess the performance of two new commercially available kits and a new in-house assay: an\n            <jats:named-content content-type=\"genus-species\">Aspergillus fumigatus<\/jats:named-content>\n            enzyme-linked immunosorbent assay (ELISA) IgG kit (Bordier Affinity Products), an\n            <jats:named-content content-type=\"genus-species\">Aspergillus<\/jats:named-content>\n            Western blotting IgG kit (LDBio Diagnostics), and a new in-house time-resolved fluorometric IgE assay (dissociation-enhanced lanthanide fluorescent immunoassay, or DELFIA) using recombinant proteins from an\n            <jats:named-content content-type=\"genus-species\">Aspergillus<\/jats:named-content>\n            sp. recently developed by our laboratory for ABPA diagnosis in a retrospective study that included 26 cystic fibrosis patients.\n            <jats:named-content content-type=\"genus-species\">Aspergillus fumigatus<\/jats:named-content>\n            -specific IgG levels measured by a commercial ELISA kit were in accordance with the level of precipitins currently used in our lab. The ELISA kit could accelerate and help standardize ABPA diagnosis.\n            <jats:named-content content-type=\"genus-species\">Aspergillus fumigatus<\/jats:named-content>\n            -specific IgE levels measured by ImmunoCAP (Phadia) with\n            <jats:named-content content-type=\"genus-species\">A. fumigatus<\/jats:named-content>\n            M3 antigen and by DELFIA with a purified protein extract of\n            <jats:named-content content-type=\"genus-species\">A. fumigatus<\/jats:named-content>\n            were significantly correlated (\n            <jats:italic>P<\/jats:italic>\n            &lt; 10\n            <jats:sup>\u22126<\/jats:sup>\n            ). The results with recombinant antigens glucose-6-phosphate isomerase and mannitol-1-phosphate dehydrogenase were encouraging but must be confirmed with sera from more patients. The DELFIA is an effective tool that can detect specific IgE against more fungal allergens than can be detected with other commercially available tests.\n          <\/jats:p>","DOI":"10.1128\/cvi.00498-15","type":"journal-article","created":{"date-parts":[[2015,12,24]],"date-time":"2015-12-24T04:16:01Z","timestamp":1450930561000},"page":"196-203","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":16,"title":["New Commercially Available IgG Kits and Time-Resolved Fluorometric IgE Assay for Diagnosis of Allergic Bronchopulmonary Aspergillosis in Patients with Cystic Fibrosis"],"prefix":"10.1128","volume":"23","author":[{"given":"Coralie","family":"Barrera","sequence":"first","affiliation":[{"name":"UMR6249 Chrono-environnement, University of Franche-Comt\u00e9, Besan\u00e7on, France"},{"name":"Parasitology-Mycology Department, University Hospital of Besan\u00e7on, Besan\u00e7on, 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