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These platforms consisted of a recombinant chimpanzee adenoviral vector 63 (ChAd63) expressing\n            <jats:italic>Pv<\/jats:italic>\n            CelTOS (Ad), a recombinant modified vaccinia virus Ankara expressing\n            <jats:italic>Pv<\/jats:italic>\n            CelTOS (MVA),\n            <jats:italic>Pv<\/jats:italic>\n            CelTOS conjugated to bacteriophage Q\u03b2 virus-like particles (VLPs), and a recombinant\n            <jats:italic>Pv<\/jats:italic>\n            CelTOS protein expressed in eukaryotic HEK293T cells (protein). Inbred BALB\/c mice and outbred CD-1 mice were immunized using the following prime-boost regimens: Ad-MVA, Ad-VLPs, and Ad-protein. Protective efficacy against sporozoite challenge was assessed after immunization using a novel chimeric rodent\n            <jats:named-content content-type=\"genus-species\">Plasmodium berghei<\/jats:named-content>\n            parasite (\n            <jats:italic>Pb-Pv<\/jats:italic>\n            CelTOS). This chimeric parasite expresses\n            <jats:named-content content-type=\"genus-species\">P. vivax<\/jats:named-content>\n            CelTOS in place of the endogenous\n            <jats:named-content content-type=\"genus-species\">P. berghei<\/jats:named-content>\n            CelTOS and produces fully infectious sporozoites. A single Ad immunization in BALB\/c and CD-1 mice induced anti-\n            <jats:italic>Pv<\/jats:italic>\n            CelTOS antibodies which were boosted efficiently using MVA, VLP, or protein immunization.\n            <jats:italic>Pv<\/jats:italic>\n            CelTOS-specific gamma interferon- and tumor necrosis factor alpha-producing CD8\n            <jats:sup>+<\/jats:sup>\n            T cells were induced at high frequencies by all prime-boost regimens in BALB\/c mice but not in CD-1 mice; in CD-1 mice, they were only marginally increased after boosting with MVA. Despite the induction of anti-\n            <jats:italic>Pv<\/jats:italic>\n            CelTOS antibodies and\n            <jats:italic>Pv<\/jats:italic>\n            CelTOS-specific CD8\n            <jats:sup>+<\/jats:sup>\n            T-cell responses, only low levels of protective efficacy against challenge with\n            <jats:italic>Pb-Pv<\/jats:italic>\n            CelTOS sporozoites were obtained using any immunization strategy. In BALB\/c mice, no immunization regimens provided significant protection against a\n            <jats:italic>Pb-Pv<\/jats:italic>\n            CelTOS chimeric sporozoite challenge. In CD-1 mice, modest protective efficacy against challenge with chimeric\n            <jats:named-content content-type=\"genus-species\">P. berghei<\/jats:named-content>\n            sporozoites expressing either\n            <jats:italic>Pv<\/jats:italic>\n            CelTOS or\n            <jats:named-content content-type=\"genus-species\">P. falciparum<\/jats:named-content>\n            CelTOS was observed using the Ad-protein vaccination regimen.\n          <\/jats:p>","DOI":"10.1128\/cvi.00501-16","type":"journal-article","created":{"date-parts":[[2017,2,9]],"date-time":"2017-02-09T01:50:25Z","timestamp":1486605025000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":26,"title":["Evaluation of Plasmodium vivax Cell-Traversal Protein for Ookinetes and Sporozoites as a Preerythrocytic P. vivax Vaccine"],"prefix":"10.1128","volume":"24","author":[{"given":"Eduardo","family":"Alves","sequence":"first","affiliation":[{"name":"The Jenner Institute, Nuffield Department of Medicine, University of Oxford, Oxford, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmed M.","family":"Salman","sequence":"additional","affiliation":[{"name":"The Jenner Institute, Nuffield Department of Medicine, University of Oxford, Oxford, United Kingdom"},{"name":"Leiden Malaria Research Group, Department of Parasitology, Center of Infectious Diseases, Leiden University Medical Center, Leiden, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabiana","family":"Leoratti","sequence":"additional","affiliation":[{"name":"The Jenner Institute, Nuffield Department of Medicine, University of Oxford, Oxford, United Kingdom"},{"name":"Immunology, RIA, Inselspital, University of Bern, Bern, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cesar","family":"Lopez-Camacho","sequence":"additional","affiliation":[{"name":"The Jenner Institute, Nuffield Department of Medicine, University of Oxford, Oxford, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martha Eva","family":"Viveros-Sandoval","sequence":"additional","affiliation":[{"name":"Laboratorio de Hemostasia y Biolog\u00eda Vascular, Divisi\u00f3n de Estudios de Posgrado, Facultad de Ciencias M\u00e9dicas y Biol\u00f3gicas Dr. Ignacio Ch\u00e1vez, Universidad Michoacana de San Nicol\u00e1s de Hidalgo, Morelia, Michoac\u00e1n, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amar","family":"Lall","sequence":"additional","affiliation":[{"name":"The Jenner Institute, Nuffield Department of Medicine, University of Oxford, Oxford, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aadil","family":"El-Turabi","sequence":"additional","affiliation":[{"name":"The Jenner Institute, Nuffield Department of Medicine, University of Oxford, Oxford, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin F.","family":"Bachmann","sequence":"additional","affiliation":[{"name":"The Jenner Institute, Nuffield Department of Medicine, University of Oxford, Oxford, United Kingdom"},{"name":"Immunology, RIA, Inselspital, University of Bern, Bern, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adrian V. 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