{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T13:15:42Z","timestamp":1789996542364,"version":"4.0.1"},"reference-count":25,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2010,4,1]],"date-time":"2010-04-01T00:00:00Z","timestamp":1270080000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Vaccine Immunol"],"published-print":{"date-parts":[[2010,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Taeniasis\/cysticercosis caused by\n            <jats:italic>Taenia solium<\/jats:italic>\n            is a frequent parasitic infection of the human brain in most of the world. Rapid and simple screening tools to identify taeniasis and cysticercosis cases are needed for control programs, mostly to identify tapeworm carriers which are the source of infection and need to be treated, or as tools for point-of-care case detection or confirmation. These screening assays should be affordable, reliable, rapid, and easy to perform. Immunochromatographic tests meet these criteria. To demonstrate proof of principle, we developed and evaluated two magnetic immunochromatographic tests (MICTs) for detection of human\n            <jats:italic>Taenia solium<\/jats:italic>\n            taeniasis antibodies (ES33-MICT) and neurocysticercosis antibodies (T24-MICT). These assays detected stage-specific antibodies by using two recombinant proteins, rES33 for detection of taeniasis antibodies and rT24H for detection of cysticercosis antibodies. The sensitivity and specificity of the ES33-MICT to detect taeniasis infections were 94.5% and 96%, respectively, and those of the T24-MICT to detect cases of human cysticercosis with two or more viable brain cysts were 93.9% and 98.9%, respectively. These data provide proof of principle that the ES33- and T24-MICTs provide rapid and suitable methods to identify individuals with taeniasis and cysticercosis.\n          <\/jats:p>","DOI":"10.1128\/cvi.00511-09","type":"journal-article","created":{"date-parts":[[2010,2,25]],"date-time":"2010-02-25T04:53:32Z","timestamp":1267073612000},"page":"631-637","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":63,"title":["Development and Evaluation of a Magnetic Immunochromatographic Test To Detect\n            <i>Taenia solium<\/i>\n            , Which Causes Taeniasis and Neurocysticercosis in Humans"],"prefix":"10.1128","volume":"17","author":[{"given":"Sukwan","family":"Handali","sequence":"first","affiliation":[{"name":"Division of Parasitic Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia"},{"name":"Pacific BioPharm, San Diego, California"},{"name":"MagnaBioSciences, San Diego, California"},{"name":"Cysticercosis Unit, Instituto de Ciencias Neurologicas, Lima, Peru"},{"name":"School of Veterinary Medicine, Universidad de San Marcos, Lima, Peru"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Molly","family":"Klarman","sequence":"additional","affiliation":[{"name":"Division of Parasitic Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia"},{"name":"Pacific BioPharm, San Diego, California"},{"name":"MagnaBioSciences, San Diego, California"},{"name":"Cysticercosis Unit, Instituto de Ciencias Neurologicas, Lima, Peru"},{"name":"School of Veterinary Medicine, Universidad de San Marcos, Lima, Peru"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amanda N.","family":"Gaspard","sequence":"additional","affiliation":[{"name":"Division of Parasitic Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia"},{"name":"Pacific BioPharm, San Diego, California"},{"name":"MagnaBioSciences, San Diego, California"},{"name":"Cysticercosis Unit, Instituto de Ciencias Neurologicas, Lima, Peru"},{"name":"School of Veterinary Medicine, Universidad de San Marcos, Lima, Peru"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"X. Fan","family":"Dong","sequence":"additional","affiliation":[{"name":"Division of Parasitic Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia"},{"name":"Pacific BioPharm, San Diego, California"},{"name":"MagnaBioSciences, San Diego, California"},{"name":"Cysticercosis Unit, Instituto de Ciencias Neurologicas, Lima, Peru"},{"name":"School of Veterinary Medicine, Universidad de San Marcos, Lima, Peru"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ronald","family":"LaBorde","sequence":"additional","affiliation":[{"name":"Division of Parasitic Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia"},{"name":"Pacific BioPharm, San Diego, California"},{"name":"MagnaBioSciences, San Diego, California"},{"name":"Cysticercosis Unit, Instituto de Ciencias Neurologicas, Lima, Peru"},{"name":"School of Veterinary Medicine, Universidad de San Marcos, Lima, Peru"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John","family":"Noh","sequence":"additional","affiliation":[{"name":"Division of Parasitic Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia"},{"name":"Pacific BioPharm, San Diego, California"},{"name":"MagnaBioSciences, San Diego, California"},{"name":"Cysticercosis Unit, Instituto de Ciencias Neurologicas, Lima, Peru"},{"name":"School of Veterinary Medicine, Universidad de San Marcos, Lima, Peru"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yeuk-Mui","family":"Lee","sequence":"additional","affiliation":[{"name":"Division of Parasitic Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia"},{"name":"Pacific BioPharm, San Diego, California"},{"name":"MagnaBioSciences, San Diego, California"},{"name":"Cysticercosis Unit, Instituto de Ciencias Neurologicas, Lima, Peru"},{"name":"School of Veterinary Medicine, Universidad de San Marcos, Lima, Peru"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvia","family":"Rodriguez","sequence":"additional","affiliation":[{"name":"Division of Parasitic Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia"},{"name":"Pacific BioPharm, San Diego, California"},{"name":"MagnaBioSciences, San Diego, California"},{"name":"Cysticercosis Unit, Instituto de Ciencias Neurologicas, Lima, Peru"},{"name":"School of Veterinary Medicine, Universidad de San Marcos, Lima, Peru"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Armando E.","family":"Gonzalez","sequence":"additional","affiliation":[{"name":"Division of Parasitic Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia"},{"name":"Pacific BioPharm, San Diego, California"},{"name":"MagnaBioSciences, San Diego, California"},{"name":"Cysticercosis Unit, Instituto de Ciencias Neurologicas, Lima, Peru"},{"name":"School of Veterinary Medicine, Universidad de San Marcos, Lima, Peru"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hector H.","family":"Garcia","sequence":"additional","affiliation":[{"name":"Division of Parasitic Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia"},{"name":"Pacific BioPharm, San Diego, California"},{"name":"MagnaBioSciences, San Diego, California"},{"name":"Cysticercosis Unit, Instituto de Ciencias Neurologicas, Lima, Peru"},{"name":"School of Veterinary Medicine, Universidad de San Marcos, Lima, Peru"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert H.","family":"Gilman","sequence":"additional","affiliation":[{"name":"Division of Parasitic Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia"},{"name":"Pacific BioPharm, San Diego, California"},{"name":"MagnaBioSciences, San Diego, California"},{"name":"Cysticercosis Unit, Instituto de Ciencias Neurologicas, Lima, Peru"},{"name":"School of Veterinary Medicine, Universidad de San Marcos, Lima, Peru"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Victor C. W.","family":"Tsang","sequence":"additional","affiliation":[{"name":"Division of Parasitic Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia"},{"name":"Pacific BioPharm, San Diego, California"},{"name":"MagnaBioSciences, San Diego, California"},{"name":"Cysticercosis Unit, Instituto de Ciencias Neurologicas, Lima, Peru"},{"name":"School of Veterinary Medicine, Universidad de San Marcos, Lima, Peru"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricia P.","family":"Wilkins","sequence":"additional","affiliation":[{"name":"Division of Parasitic Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia"},{"name":"Pacific BioPharm, San Diego, California"},{"name":"MagnaBioSciences, San Diego, California"},{"name":"Cysticercosis Unit, Instituto de Ciencias Neurologicas, Lima, Peru"},{"name":"School of Veterinary Medicine, Universidad de San Marcos, Lima, Peru"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1016\/S0001-706X(03)00059-7","volume":"87","year":"2003","unstructured":"Allan, J. C., P. P. Wilkins, V. C. Tsang, and P. S. Craig. 2003. Immunodiagnostic tools for taeniasis. Acta Trop.87:87-93.","journal-title":"Acta Trop."},{"key":"e_1_3_2_3_2","unstructured":"Practical statistics for medical research. 1993"},{"key":"e_1_3_2_4_2","doi-asserted-by":"crossref","first-page":"278","DOI":"10.4269\/ajtmh.2005.72.278","volume":"72","year":"2005","unstructured":"Bueno, E. C., C. M. Scheel, A. J. Vaz, L. R. Machado, J. A. Livramento, O. M. Takayanagui, V. C. Tsang, and K. Hancock. 2005. Application of synthetic 8-kD and recombinant GP50 antigens in the diagnosis of neurocysticercosis by enzyme-linked immunosorbent assay. Am. J. Trop. Med. Hyg.72:278-283.","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"e_1_3_2_5_2","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1212\/WNL.57.2.177","volume":"57","year":"2001","unstructured":"Del Brutto, O. H., V. Rajshekhar, A. C. White, Jr., V. C. Tsang, T. E. Nash, O. M. Takayanagui, P. M. Schantz, C. A. Evans, A. Flisser, D. Correa, D. Botero, J. C. Allan, E. Sarti, A. E. Gonzalez, R. H. Gilman, and H. H. Garcia. 2001. Proposed diagnostic criteria for neurocysticercosis. Neurology57:177-183.","journal-title":"Neurology"},{"key":"e_1_3_2_6_2","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1038\/nature05441","volume":"444","year":"2006","unstructured":"Girosi, F., S. S. Olmsted, E. Keeler, D. C. Hay Burgess, Y. W. Lim, J. E. Aledort, M. E. Rafael, K. A. Ricci, R. Boer, L. Hilborne, K. P. Derose, M. V. Shea, C. M. Beighley, C. A. Dahl, and J. Wasserman. 2006. Developing and interpreting models to improve diagnostics in developing countries. Nature444(Suppl. 1):3-8.","journal-title":"Nature"},{"key":"e_1_3_2_7_2","doi-asserted-by":"crossref","first-page":"847","DOI":"10.4269\/ajtmh.1994.51.847","volume":"51","year":"1994","unstructured":"Gonzalez, A. E., R. Gilman, H. H. Garcia, J. McDonald, K. Kacena, V. C. Tsang, J. B. Pilcher, F. Suarez, C. Gavidia, and E. Miranda. 1994. Use of sentinel pigs to monitor environmental Taenia solium contamination. The Cysticercosis Working Group in Peru (CWG). Am. J. Trop. Med. Hyg.51:847-850.","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.6.2577-2586.2003"},{"key":"e_1_3_2_9_2","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1016\/j.molbiopara.2003.10.001","volume":"133","year":"2004","unstructured":"Hancock, K., S. Pattabhi, R. M. Greene, M. L. Yushak, F. Williams, A. Khan, J. W. Priest, M. Z. Levine, and V. C. Tsang. 2004. Characterization and cloning of GP50, a Taenia solium antigen diagnostic for cysticercosis. Mol. Biochem. Parasitol.133:115-124.","journal-title":"Mol. Biochem. Parasitol."},{"key":"e_1_3_2_10_2","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/j.molbiopara.2006.02.004","volume":"147","year":"2006","unstructured":"Hancock, K., S. Pattabhi, F. W. Whitfield, M. L. Yushak, W. S. Lane, H. H. Garcia, A. E. Gonzalez, R. H. Gilman, and V. C. Tsang. 2006. Characterization and cloning of T24, a Taenia solium antigen diagnostic for cysticercosis. Mol. Biochem. Parasitol.147:109-117.","journal-title":"Mol. Biochem. Parasitol."},{"key":"e_1_3_2_11_2","doi-asserted-by":"crossref","first-page":"322","DOI":"10.4269\/ajtmh.2004.71.322","volume":"71","year":"2004","unstructured":"Handali, S., A. E. Gonzalez, K. Hancock, H. H. Garcia, J. M. Roberts, R. H. Gilman, and V. C. Tsang. 2004. Porcine antibody responses to taenia solium antigens rGp50 and sTs18var1. Am. J. Trop. Med. Hyg.71:322-326.","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00339-09"},{"key":"e_1_3_2_13_2","first-page":"36","volume":"8","year":"2002","unstructured":"LaBorde, R. T., and B. O'Farrell. 2002. Paramagnetic-particle detection in lateral-flow assays. IVD Technol.8:36-41.","journal-title":"IVD Technol."},{"key":"e_1_3_2_14_2","doi-asserted-by":"crossref","first-page":"631","DOI":"10.1645\/GE-189R","volume":"90","year":"2004","unstructured":"Levine, M. Z., J. C. Calderon, P. P. Wilkins, W. S. Lane, J. M. Asara, K. Hancock, A. E. Gonzalez, H. H. Garcia, R. H. Gilman, and V. C. Tsang. 2004. Characterization, cloning, and expression of two diagnostic antigens for Taenia solium tapeworm infection. J. Parasitol.90:631-638.","journal-title":"J. Parasitol."},{"key":"e_1_3_2_15_2","doi-asserted-by":"crossref","first-page":"409","DOI":"10.1645\/GE-938R.1","volume":"93","year":"2007","unstructured":"Levine, M. Z., M. M. Lewis, S. Rodriquez, J. A. Jimenez, A. Khan, S. Lin, H. H. Garcia, A. E. Gonzales, R. H. Gilman, and V. C. Tsang. 2007. Development of an enzyme-linked immunoelectrotransfer blot (EITB) assay using two baculovirus expressed recombinant antigens for diagnosis of Taenia solium taeniasis. J. Parasitol.93:409-417.","journal-title":"J. Parasitol."},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.1.133-137.2000"},{"key":"e_1_3_2_17_2","first-page":"41","volume":"12","year":"2006","unstructured":"O'Farrell, B., and J. Bauer. 2006. Developing highly sensitive, more-reproducible lateral-flow assays. IVD Technol.12:41-49.","journal-title":"IVD Technol."},{"key":"e_1_3_2_18_2","doi-asserted-by":"crossref","first-page":"2170","DOI":"10.1373\/clinchem.2006.072884","volume":"52","year":"2006","unstructured":"Peck, R. B., J. Schweizer, B. H. Weigl, C. Somoza, J. Silver, J. W. Sellors, and P. S. Lu. 2006. A magnetic immunochromatographic strip test for detection of human papillomavirus 16 E6. Clin. Chem.52:2170-2172.","journal-title":"Clin. Chem."},{"key":"e_1_3_2_19_2","first-page":"397","volume":"27","year":"1993","unstructured":"Schantz, P. M., M. Cruz, E. Sarti, and Z. Pawlowski. 1993. Potential eradicability of taeniasis and cysticercosis. Bull. Pan Am. Health Organ.27:397-403.","journal-title":"Bull. Pan Am. Health Organ."},{"key":"e_1_3_2_20_2","doi-asserted-by":"crossref","first-page":"771","DOI":"10.4269\/ajtmh.2005.73.771","volume":"73","year":"2005","unstructured":"Scheel, C. M., A. Khan, K. Hancock, H. H. Garcia, A. E. Gonzalez, R. H. Gilman, and V. C. Tsang. 2005. Serodiagnosis of neurocysticercosis using synthetic 8-kD proteins: comparison of assay formats. Am. J. Trop. Med. Hyg.73:771-776.","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"e_1_3_2_21_2","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1093\/infdis\/159.1.50","volume":"159","year":"1989","unstructured":"Tsang, V. C., J. A. Brand, and A. E. Boyer. 1989. An enzyme-linked immunoelectrotransfer blot assay and glycoprotein antigens for diagnosing human cysticercosis (Taenia solium). J. Infect. Dis.159:50-59.","journal-title":"J. Infect. Dis."},{"key":"e_1_3_2_22_2","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1038\/nature05448","volume":"444","year":"2006","unstructured":"Urdea, M., L. A. Penny, S. S. Olmsted, M. Y. Giovanni, P. Kaspar, A. Shepherd, P. Wilson, C. A. Dahl, S. Buchsbaum, G. Moeller, and D. C. Hay Burgess. 2006. Requirements for high impact diagnostics in the developing world. Nature444(Suppl. 1):73-79.","journal-title":"Nature"},{"key":"e_1_3_2_23_2","doi-asserted-by":"crossref","first-page":"591","DOI":"10.1016\/j.clinbiochem.2005.03.008","volume":"38","year":"2005","unstructured":"von Lode, P. 2005. Point-of-care immunotesting: approaching the analytical performance of central laboratory methods. Clin. Biochem.38:591-606.","journal-title":"Clin. Biochem."},{"key":"e_1_3_2_24_2","doi-asserted-by":"crossref","first-page":"199","DOI":"10.4269\/ajtmh.1999.60.199","volume":"60","year":"1999","unstructured":"Wilkins, P. P., J. C. Allan, M. Verastegui, M. Acosta, A. G. Eason, H. H. Garcia, A. E. Gonzalez, R. H. Gilman, and V. C. Tsang. 1999. Development of a serologic assay to detect Taenia solium taeniasis. Am. J. Trop. Med. Hyg.60:199-204.","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"e_1_3_2_25_2","doi-asserted-by":"crossref","first-page":"1007","DOI":"10.1093\/infdis\/164.5.1007","volume":"164","year":"1991","unstructured":"Wilson, M., R. T. Bryan, J. A. Fried, D. A. Ware, P. M. Schantz, J. B. Pilcher, and V. C. Tsang. 1991. Clinical evaluation of the cysticercosis enzyme-linked immunoelectrotransfer blot in patients with neurocysticercosis. J. Infect. Dis.164:1007-1009.","journal-title":"J. Infect. Dis."},{"key":"e_1_3_2_26_2","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1016\/j.jviromet.2009.04.003","volume":"160","year":"2009","unstructured":"Workman, S., S. K. Wells, C. P. Pau, S. M. Owen, X. F. Dong, R. LaBorde, and T. C. Granade. 2009. Rapid detection of HIV-1 p24 antigen using magnetic immuno-chromatography (MICT). J. Virol. Methods160:14-21.","journal-title":"J. Virol. Methods"}],"container-title":["Clinical and Vaccine Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/CVI.00511-09","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/CVI.00511-09","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,7,29]],"date-time":"2021-07-29T20:43:41Z","timestamp":1627591421000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/CVI.00511-09"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,4]]},"references-count":25,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2010,4]]}},"alternative-id":["10.1128\/CVI.00511-09"],"URL":"https:\/\/doi.org\/10.1128\/cvi.00511-09","relation":{},"ISSN":["1556-6811","1556-679X"],"issn-type":[{"value":"1556-6811","type":"print"},{"value":"1556-679X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,4]]},"assertion":[{"value":"2009-12-14","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2010-02-12","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-12-18","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}