{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T13:19:06Z","timestamp":1760015946794},"reference-count":44,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2010,4,1]],"date-time":"2010-04-01T00:00:00Z","timestamp":1270080000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Vaccine Immunol"],"published-print":{"date-parts":[[2010,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:italic>Taenia saginata<\/jats:italic>\n            metacestode antigens have been constituted a useful alternative antigen for neurocysticercosis (NC) serodiagnosis, particularly due to an increasing difficulty to obtain\n            <jats:italic>Taenia solium<\/jats:italic>\n            homologous antigen. Cross-reactivity with\n            <jats:italic>Echinococcus granulosus<\/jats:italic>\n            infection occurs in homologous and heterologous antigens and could be avoided by using different purified methods. The present study evaluated antigen fractions obtained from saline extracts of\n            <jats:italic>T. saginata<\/jats:italic>\n            metacestodes purified by affinity chromatography with jacalin or concanavalin A (ConA) lectins to detect IgG antibodies by enzyme-linked immunosorbent assay (ELISA) and immunoblot analysis to diagnose human NC. Serum samples were collected from 142 individuals: 40 of them were diagnosed with NC, 62 presented\n            <jats:italic>Taenia<\/jats:italic>\n            sp. and other parasites, and 40 were apparently healthy individuals. The jacalin- and ConA-unbound fractions demonstrated sensitivity and specificity higher than those of bound fractions. Among unbound fractions, ConA demonstrated statistically higher sensitivity and specificity by ELISA (90% and 93.1%, respectively). By immunoblot assay, the 64- to 68-kDa component from the ConA-unbound fraction showed 100% sensitivity and specificity, making this component suitable for use as a specific antigen for diagnosis of NC. To our knowledge, this is the first report showing the relevance of using the unbound ConA fraction of\n            <jats:italic>T. saginata<\/jats:italic>\n            metacestodes to diagnose NC. In conclusion, the results obtained herein clearly demonstrate that antigenic fractions without affinity to ConA, obtained from\n            <jats:italic>T. saginata<\/jats:italic>\n            metacestodes, are an important source of specific peptides and are efficient in the diagnosis of NC when tested by immunoblot assay.\n          <\/jats:p>","DOI":"10.1128\/cvi.00516-09","type":"journal-article","created":{"date-parts":[[2010,2,4]],"date-time":"2010-02-04T02:56:55Z","timestamp":1265252215000},"page":"638-644","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":11,"title":["<i>Taenia saginata<\/i>\n            Metacestode Antigenic Fractions without Affinity to Concanavalin A Are an Important Source of Specific Antigens for the Diagnosis of Human Neurocysticercosis"],"prefix":"10.1128","volume":"17","author":[{"given":"Heliana B.","family":"Oliveira","sequence":"first","affiliation":[{"name":"Departamento de Imunologia, Microbiologia e Parasitologia, Instituto de Cie\u0302ncias Biome\u0301dicas, Universidade Federal de Uberla\u0302ndia, Uberla\u0302ndia, Minas Gerais, Brazil"},{"name":"Departamento de Cie\u0302ncias Biolo\u0301gicas, Universidade Federal de Goia\u0301s, Campus Catala\u0303o, Catala\u0303o, Goia\u0301s, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gleyce A.","family":"Machado","sequence":"additional","affiliation":[{"name":"Departamento de Imunologia, Microbiologia e Parasitologia, Instituto de Cie\u0302ncias Biome\u0301dicas, Universidade Federal de Uberla\u0302ndia, Uberla\u0302ndia, Minas Gerais, Brazil"},{"name":"Departamento de Cie\u0302ncias Biolo\u0301gicas, Universidade Federal de Goia\u0301s, Campus Catala\u0303o, Catala\u0303o, Goia\u0301s, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jose\u0301 R.","family":"Mineo","sequence":"additional","affiliation":[{"name":"Departamento de Imunologia, Microbiologia e Parasitologia, Instituto de Cie\u0302ncias Biome\u0301dicas, Universidade Federal de Uberla\u0302ndia, Uberla\u0302ndia, Minas Gerais, Brazil"},{"name":"Departamento de Cie\u0302ncias Biolo\u0301gicas, Universidade Federal de Goia\u0301s, Campus Catala\u0303o, Catala\u0303o, Goia\u0301s, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julia M.","family":"Costa-Cruz","sequence":"additional","affiliation":[{"name":"Departamento de Imunologia, Microbiologia e Parasitologia, Instituto de Cie\u0302ncias Biome\u0301dicas, Universidade Federal de Uberla\u0302ndia, Uberla\u0302ndia, Minas Gerais, Brazil"},{"name":"Departamento de Cie\u0302ncias Biolo\u0301gicas, Universidade Federal de Goia\u0301s, Campus Catala\u0303o, Catala\u0303o, Goia\u0301s, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","unstructured":"Eine einfache Methode zur Auffindung von Ankylostomum (Nematoden) Larven in Erdproben 41-47. 1917"},{"key":"e_1_3_2_3_2","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1046\/j.1365-3024.1998.00117.x","volume":"20","year":"1998","unstructured":"Barbieri, M., V. 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