{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,5,1]],"date-time":"2024-05-01T08:34:18Z","timestamp":1714552458277},"reference-count":44,"publisher":"American Society for Microbiology","issue":"7","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clin. Vaccine Immunol."],"published-print":{"date-parts":[[2011,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Leprosy is an infectious disease caused by<jats:named-content content-type=\"genus-species\">Mycobacterium leprae<\/jats:named-content>, which is a noncultivable bacterium. One of the principal goals of leprosy research is to develop serological tests that will allow identification and early treatment of leprosy patients.<jats:named-content content-type=\"genus-species\">M. habana<\/jats:named-content>is a cultivable nonpathogenic mycobacterium and candidate vaccine for leprosy, and several antigens that cross-react between<jats:named-content content-type=\"genus-species\">M. leprae<\/jats:named-content>and<jats:named-content content-type=\"genus-species\">M. habana<\/jats:named-content>have been discovered. The aim of the present study was to extend the identification of cross-reactive antigens by identifying<jats:named-content content-type=\"genus-species\">M. habana<\/jats:named-content>proteins that reacted by immunoblotting with antibodies in serum samples from leprosy patients but not with antibodies in sera from tuberculosis (TB) patients or healthy donors (HDs). A 28-kDa antigen that specifically reacted with sera from leprosy patients was identified. To further characterize this antigen, protein spots were aligned in two-dimensional polyacrylamide gels and Western blots. Spots cut out from the gels were then analyzed by mass spectrometry. Two proteins were identified: enoyl-coenzyme A hydratase (lipid metabolism; ML2498) and antigen 85B (Ag85B; mycolyltransferase; ML2028). These proteins represent promising candidates for the design of a reliable tool for the serodiagnosis of lepromatous leprosy, which is the most frequent form in Mexico.<\/jats:p>","DOI":"10.1128\/cvi.00519-10","type":"journal-article","created":{"date-parts":[[2011,5,26]],"date-time":"2011-05-26T05:26:44Z","timestamp":1306387604000},"page":"1097-1103","source":"Crossref","is-referenced-by-count":5,"title":["Enoyl-Coenzyme A Hydratase and Antigen 85B of Mycobacterium habana Are Specifically Recognized by Antibodies in Sera from Leprosy Patients"],"prefix":"10.1128","volume":"18","author":[{"given":"J.","family":"Seraf\u00edn-L\u00f3pez","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Talavera-Paulin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. C.","family":"Amador-Molina","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Alvarado-River\u00f3n","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. M.","family":"Vilchis-Landeros","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.","family":"M\u00e9ndez-Ortega","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Fafutis-Morris","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"V.","family":"Paredes-Cervantes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"L\u00f3pez-Santiago","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C. I.","family":"Le\u00f3n","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. I.","family":"Guerrero","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. M.","family":"Ribas-Aparicio","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G.","family":"Mendoza-Hern\u00e1ndez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Carre\u00f1o-Mart\u00ednez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Estrada-Parra","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I.","family":"Estrada-Garc\u00eda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","published-online":{"date-parts":[[2011,5,25]]},"reference":[{"key":"key20180919073520_B1","first-page":"45","article-title":"Methods for identification","volume-title":"Mycobacteria: isolation, identification and sensitivity testing","author":"Allen","year":"1968"},{"key":"key20180919073520_B2","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1128\/IAI.74.1.175-182.2006","article-title":"Antigen discovery: a postgenomic approach to leprosy diagnosis","volume":"74","author":"Ar\u00e1oz","year":"2006","journal-title":"Infect. Immun."},{"key":"key20180919073520_B3","doi-asserted-by":"crossref","first-page":"4800","DOI":"10.1021\/ac990448m","article-title":"Toward a clinical molecular scanner for proteome research: parallel protein chemical processing before and during Western blot","volume":"71","author":"Bienvenut","year":"1999","journal-title":"Anal. Chem."},{"key":"key20180919073520_B4","doi-asserted-by":"crossref","first-page":"1375","DOI":"10.1099\/13500872-142-6-1375","article-title":"A major T-cell-inducing cytosolic 23 kDa protein antigen of the vaccine candidate Mycobacterium habana is superoxide dismutase","volume":"142","author":"Bisht","year":"1996","journal-title":"Microbiology"},{"key":"key20180919073520_B5","doi-asserted-by":"crossref","first-page":"133","DOI":"10.4269\/ajtmh.1998.58.133","article-title":"A simple dipstick assay for the detection of antibodies to phenolic glycolipid-I of Mycobacterium leprae","volume":"58","author":"B\u00fchrer","year":"1998","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"key20180919073520_B6","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1111\/j.1574-695X.1999.tb01382.x","article-title":"Secretory proteins of Mycobacterium habana induce a protective immune response against experimental tuberculosis","volume":"26","author":"Chaturvedi","year":"1999","journal-title":"FEMS Immunol. Med. Microbiol."},{"key":"key20180919073520_B7","first-page":"31","article-title":"Immunoreactive antigens of a candidate leprosy vaccine: Mycobacterium habana","volume":"66","author":"Chaturvedi","year":"1995","journal-title":"Lepr. Rev."},{"key":"key20180919073520_B8","doi-asserted-by":"crossref","first-page":"502","DOI":"10.1046\/j.1365-3083.2000.00722.x","article-title":"Mechanisms involved in protective immune response generated by secretory proteins of Mycobacterium habana against experimental tuberculosis","volume":"51","author":"Divya Jyothi","year":"2000","journal-title":"Scand. J. Immunol."},{"key":"key20180919073520_B9","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1016\/0022-1759(91)90330-I","article-title":"An isoelectric focusing method for the study of the humoral response against the antigen 85 complex of Mycobacterium bovis BCG in the different forms of leprosy","volume":"145","author":"Drowart","year":"1991","journal-title":"J. Immunol. Methods"},{"key":"key20180919073520_B10","first-page":"641","article-title":"A longitudinal study of the incidence of leprosy in a hyperendemic area in Zaire, with special reference to PGL-antibody results. The Yalisombo Study Group","volume":"58","author":"Groenen","year":"1990","journal-title":"Int. J. Lepr. Other Mycobact. Dis."},{"key":"key20180919073520_B11","first-page":"695","article-title":"Protective effect of Mycobacterium habana in mice against infection with indigenous strains of M. tuberculosis","volume":"53","author":"Gupta","year":"1984","journal-title":"Curr. Sci."},{"key":"key20180919073520_B12","first-page":"1190","article-title":"Mycobacterium habana, a new immunogenic strain in experimental tuberculosis of mice","volume":"17","author":"Gupta","year":"1979","journal-title":"Indian J. Exp. Biol."},{"key":"key20180919073520_B13","doi-asserted-by":"crossref","first-page":"1755","DOI":"10.1128\/CVI.00148-08","article-title":"Serological diagnosis of leprosy in patients in Vietnam by enzyme-linked immunosorbent assay with Mycobacterium leprae-derived major membrane protein II","volume":"15","author":"Kai","year":"2008","journal-title":"Clin. Vaccine Immunol."},{"key":"key20180919073520_B14","doi-asserted-by":"crossref","first-page":"12333","DOI":"10.1074\/jbc.271.21.12333","article-title":"Novel O-methylated terminal glucuronic acid characterizes the polar glycopeptidolipids of Mycobacterium habana strain TMC 5135","volume":"271","author":"Khoo","year":"1996","journal-title":"J. Biol. Chem."},{"key":"key20180919073520_B15","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1038\/227680a0","article-title":"Cleavage of structural proteins during the assembly of the head of bacteriophage T4","volume":"227","author":"Laemmli","year":"1970","journal-title":"Nature"},{"key":"key20180919073520_B16","doi-asserted-by":"crossref","first-page":"1922","DOI":"10.4049\/jimmunol.144.5.1922","article-title":"The specific 18-kilodalton antigen of Mycobacterium leprae is present in Mycobacterium habana and functions as a heat-shock protein","volume":"144","author":"Lamb","year":"1990","journal-title":"J. Immunol."},{"key":"key20180919073520_B17","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1128\/IAI.63.1.88-93.1995","article-title":"Fibronectin-binding antigen 85 and the 10-kilodalton GroES-related heat shock protein are the predominant TH-1 response inducers in leprosy contacts","volume":"63","author":"Launois","year":"1995","journal-title":"Infect. Immun."},{"key":"key20180919073520_B18","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1111\/j.1365-2249.1994.tb06235.x","article-title":"T cell reactivity against antigen 85 but not against the 18- and 65-kD heat shock proteins in the early stages of acquired immunity against Mycobacterium leprae","volume":"96","author":"Launois","year":"1994","journal-title":"Clin. Exp. Immunol."},{"key":"key20180919073520_B19","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1016\/S0021-9258(19)52451-6","article-title":"Protein measurement with the Folin phenol reagent","volume":"193","author":"Lowry","year":"1951","journal-title":"J. Biol. Chem."},{"key":"key20180919073520_B20","first-page":"196","article-title":"Relationship between Mycobacterium simiae and Mycobacterium habana","volume":"111","author":"Meissner","year":"1975","journal-title":"Am. Rev. Respir. Dis."},{"key":"key20180919073520_B21","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1590\/S0037-86822008000700004","article-title":"Leprosy serology using PGL-I: a systematic review","volume":"41","author":"Moura","year":"2008","journal-title":"Rev. Soc. Bras. Med. Trop."},{"key":"key20180919073520_B22","doi-asserted-by":"crossref","first-page":"795","DOI":"10.1016\/S0264-410X(99)00327-8","article-title":"The antigen 85 complex vaccine against experimental Mycobacterium leprae infection in mice","volume":"18","author":"Naito","year":"1999","journal-title":"Vaccine"},{"key":"key20180919073520_B23","first-page":"168","article-title":"Detection of antibodies toward secreted mycobacterial antigen 85 in untreated leprosy patients' sera","volume":"67","author":"Rada-Schlaefli","year":"1999","journal-title":"Int. J. Lepr. Other Mycobact. Dis."},{"key":"key20180919073520_B24","first-page":"593","article-title":"Use of non-conventional antigen: M. habana in detecting M. leprae antibodies from leprosy patients and contacts in FLA-ABS test","volume":"60","author":"Rana","year":"1988","journal-title":"Indian J. Lepr."},{"key":"key20180919073520_B25","first-page":"255","article-title":"Classification of leprosy according to immunity. A five-group system","volume":"34","author":"Ridley","year":"1966","journal-title":"Int. J. Lepr. Other Mycobact. Dis."},{"key":"key20180919073520_B26","first-page":"1106","article-title":"Recognition of phenolic glycolipid-I (Mycobacterium leprae) and sulfolipid-I (M. tuberculosis) by serum from Mexican patients with leprosy or tuberculosis","volume":"3","author":"Rojas-Espinosa","year":"1999","journal-title":"Int. J. Tuberc. Lung Dis."},{"key":"key20180919073520_B27","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1016\/j.bbapap.2006.11.016","article-title":"Rv3389C from Mycobacterium tuberculosis, a member of the (R)-specific hydratase\/dehydratase family","volume":"1774","author":"Sacco","year":"2007","journal-title":"Biochim. Biophys. Acta"},{"key":"key20180919073520_B28","first-page":"371","article-title":"Foot pad enlargement as a measure of induced immunity to Mycobacterium leprae","volume":"48","author":"Shepard","year":"1980","journal-title":"Int. J. Lepr. Other Mycobact. Dis."},{"key":"key20180919073520_B29","doi-asserted-by":"crossref","first-page":"919","DOI":"10.1128\/IAI.14.4.919-928.1976","article-title":"Immunity to Mycobacterium leprae infections in mice stimulated by M. leprae, BCG, and graft-versus-host reactions","volume":"4","author":"Shepard","year":"1976","journal-title":"Infect. Immun."},{"key":"key20180919073520_B30","doi-asserted-by":"crossref","first-page":"1096","DOI":"10.1128\/IAI.40.3.1096-1103.1983","article-title":"Comparison of the immunogenicity of vaccines prepared from viable Mycobacterium bovis BCG, heat-killed Mycobacterium leprae, and a mixture of the two for normal and M. leprae-tolerant mice","volume":"40","author":"Shepard","year":"1983","journal-title":"Infect. Immun."},{"key":"key20180919073520_B31","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1111\/j.1574-695X.2009.00617.x","article-title":"Identification of seroreactive proteins in the culture filtrate antigen of Mycobacterium avium ssp. paratuberculosis human isolates to sera from Crohn's disease patients","volume":"58","author":"Shin","year":"2010","journal-title":"FEMS Immunol. Med. Microbiol."},{"key":"key20180919073520_B32","first-page":"278","article-title":"Immunological potential of a cultivable mycobacterial strain M. habana against leprosy bacillus in mouse foot pad","volume":"57","author":"Singh","year":"1985","journal-title":"Indian J. Lepr."},{"key":"key20180919073520_B33","doi-asserted-by":"crossref","first-page":"653","DOI":"10.1128\/IAI.57.2.653-655.1989","article-title":"Vaccination of mice against Mycobacterium leprae infection","volume":"57","author":"Singh","year":"1989","journal-title":"Infect. Immun."},{"key":"key20180919073520_B34","first-page":"478","article-title":"Evaluation of diversified antigens for detection of Mycobacterium leprae antibodies from leprosy patients and contacts","volume":"32","author":"Singh","year":"1994","journal-title":"Indian J. Exp. Biol."},{"key":"key20180919073520_B35","doi-asserted-by":"crossref","first-page":"260","DOI":"10.1128\/CVI.00472-10","article-title":"Analysis of antibody responses to Mycobacterium leprae phenolic glycolipid I, lipoarabinomannan, and recombinant proteins to define disease subtype-specific antigenic profiles in leprosy","volume":"18","author":"Spencer","year":"2011","journal-title":"Clin. Vaccine Immunol."},{"key":"key20180919073520_B36","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1128\/CMR.18.1.81-101.2005","article-title":"Pathway to synthesis and processing of mycolic acids in Mycobacterium tuberculosis","volume":"18","author":"Takayama","year":"2005","journal-title":"Clin. Microbiol. Rev."},{"key":"key20180919073520_B37","doi-asserted-by":"crossref","first-page":"4350","DOI":"10.1073\/pnas.76.9.4350","article-title":"Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications","volume":"76","author":"Towbin","year":"1979","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"key20180919073520_B38","first-page":"37","article-title":"Immunogenicity induced by three strains in a BALB\/c model of progressive pulmonary tuberculosis in TB07 vaccines","author":"Vald\u00e9s","year":"2007"},{"key":"key20180919073520_B39","first-page":"38","article-title":"Virulence of M. habana strains in a BALB\/c model of progressive pulmonary tuberculosis in TB07 vaccines","author":"Vald\u00e9s","year":"2007"},{"key":"key20180919073520_B40","first-page":"65","article-title":"Mycobacterium habana: probable nueva especie dentro de las micobacterias no clasificadas","volume":"9","author":"Valdivia Alvarez","year":"1971","journal-title":"Bol. Hig. Epidemiol."},{"key":"key20180919073520_B41","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1016\/0014-5793(85)80374-4","article-title":"Prediction of sequential antigenic regions in proteins","volume":"188","author":"Welling","year":"1985","journal-title":"FEBS Lett."},{"key":"key20180919073520_B42","doi-asserted-by":"crossref","DOI":"10.1016\/j.jprot.2011.01.004","article-title":"A quantitative view on Mycobacterium leprae antigens by proteomics","author":"Wiker","year":"2011","journal-title":"J. Proteomics."},{"key":"key20180919073520_B43","doi-asserted-by":"crossref","first-page":"272","DOI":"10.1128\/IAI.58.1.272-274.1990","article-title":"Evidence for three separate genes encoding the proteins of the mycobacterial antigen 85 complex","volume":"58","author":"Wiker","year":"1990","journal-title":"Infect. Immun."},{"key":"key20180919073520_B44","doi-asserted-by":"crossref","first-page":"3332","DOI":"10.1002\/pmic.200600876","article-title":"Identification of novel bacterial plasminogen-binding proteins in the human pathogen Mycobacterium tuberculosis","volume":"7","author":"Xolalpa","year":"2007","journal-title":"Proteomics"}],"container-title":["Clinical and Vaccine Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1128\/CVI.00519-10","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,6,7]],"date-time":"2023-06-07T02:22:08Z","timestamp":1686104528000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/CVI.00519-10"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,5,25]]},"references-count":44,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2011,7,5]]},"published-print":{"date-parts":[[2011,7]]}},"alternative-id":["10.1128\/CVI.00519-10"],"URL":"https:\/\/doi.org\/10.1128\/cvi.00519-10","relation":{},"ISSN":["1556-6811","1556-679X"],"issn-type":[{"value":"1556-6811","type":"print"},{"value":"1556-679X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,5,25]]}}}