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New approaches are therefore needed to develop an effective and safe immunization strategy against this zoonotic pathogen. In this study, BALB\/c mice were subcutaneously immunized with the following: (i) the recombinant<jats:named-content content-type=\"genus-species\">Brucella<\/jats:named-content>Omp31 antigen formulated in different adjuvants (incomplete Freund adjuvant, aluminum hydroxide, Quil A, and Montanide IMS 3012 VGPR), (ii) plasmid pCIOmp31, or (iii) pCIOmp31 plasmid followed by boosting with recombinant Omp31 (rOmp31). The immune response and the protective efficacy against<jats:named-content content-type=\"genus-species\">B. canis<\/jats:named-content>infection were characterized. The different strategies induced a strong immunoglobulin G (IgG) response. Furthermore, spleen cells from rOmp31-immunized mice produced gamma interferon and interleukin-4 (IL-4) after<jats:italic>in vitro<\/jats:italic>stimulation with rOmp31, indicating the induction of a mixed Th1-Th2 response. Recombinant Omp31 administered with different adjuvants as well as the prime-boost strategy conferred protection against<jats:named-content content-type=\"genus-species\">B. canis<\/jats:named-content>. In conclusion, our results suggest that Omp31 could be a useful candidate for the development of a subcellular vaccine against<jats:named-content content-type=\"genus-species\">B. canis<\/jats:named-content>infection.<\/jats:p>","DOI":"10.1128\/cvi.00527-14","type":"journal-article","created":{"date-parts":[[2014,10,23]],"date-time":"2014-10-23T02:06:47Z","timestamp":1414030007000},"page":"1689-1694","source":"Crossref","is-referenced-by-count":25,"title":["Evaluation of the Efficacy of Outer Membrane Protein 31 Vaccine Formulations for Protection against Brucella canis in BALB\/c Mice"],"prefix":"10.1128","volume":"21","author":[{"given":"Maria","family":"Clausse","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alejandra G.","family":"D\u00edaz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andr\u00e9s E.","family":"Iba\u00f1ez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juliana","family":"Cassataro","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guillermo H.","family":"Giambartolomei","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Silvia M.","family":"Estein","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","published-online":{"date-parts":[[2014,10,22]]},"reference":[{"key":"key20180919073752_B1","doi-asserted-by":"publisher","first-page":"280","DOI":"10.1017\/S0950268809990525","article-title":"Human Brucella canis outbreak linked to infection in dogs","volume":"138","author":"Lucero","year":"2010","journal-title":"Epidemiol. 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