{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T10:05:44Z","timestamp":1789985144340,"version":"4.0.1"},"reference-count":30,"publisher":"American Society for Microbiology","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clin. Vaccine Immunol."],"published-print":{"date-parts":[[2011,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Our laboratory has developed more than a hundred mouse monoclonal antibodies (MAbs) against<jats:named-content content-type=\"genus-species\">Burkholderia pseudomallei<\/jats:named-content>and<jats:named-content content-type=\"genus-species\">Burkholderia mallei<\/jats:named-content>. These antibodies have been categorized into different groups based on their specificities and the biochemical natures of their target antigens. The current study first examined the bactericidal activities of a number of these MAbs by an<jats:italic>in vitro<\/jats:italic>opsonic assay. Then, the<jats:italic>in vivo<\/jats:italic>protective efficacy of selected MAbs was evaluated using BALB\/c mice challenged intranasally with a lethal dose of the bacteria. The opsonic assay using dimethyl sulfoxide-treated human HL-60 cells as phagocytes revealed that 19 out of 47 tested MAbs (40%) have prominent bactericidal activities against<jats:named-content content-type=\"genus-species\">B. pseudomallei<\/jats:named-content>and\/or<jats:named-content content-type=\"genus-species\">B. mallei<\/jats:named-content>. Interestingly, all MAbs with strong opsonic activities are those with specificity against either the capsular polysaccharides (PS) or the lipopolysaccharides (LPS) of the bacteria. On the other hand, none of the MAbs reacting to bacterial proteins or glycoproteins showed prominent bactericidal activity. Further study revealed that the antigenic epitopes on either the capsular PS or LPS molecules were readily available for binding in intact bacteria, while the epitopes on proteins\/glycoproteins were less accessible to the MAbs. Our<jats:italic>in vivo<\/jats:italic>study showed that four MAbs reactive to either the capsular PS or LPS were highly effective in protecting mice against lethal bacterial challenge. The result is compatible with that of our<jats:italic>in vitro<\/jats:italic>study. The MAbs with the highest protective efficacy are those reactive to either the capsular PS or LPS of the<jats:named-content content-type=\"genus-species\">Burkholderia<\/jats:named-content>bacteria.<\/jats:p>","DOI":"10.1128\/cvi.00533-10","type":"journal-article","created":{"date-parts":[[2011,3,31]],"date-time":"2011-03-31T03:40:14Z","timestamp":1301542814000},"page":"825-834","source":"Crossref","is-referenced-by-count":44,"title":["<i>In Vitro<\/i>and<i>In Vivo<\/i>Studies of Monoclonal Antibodies with Prominent Bactericidal Activity against Burkholderia pseudomallei and Burkholderia mallei"],"prefix":"10.1128","volume":"18","author":[{"given":"Shimin","family":"Zhang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shaw-Huey","family":"Feng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bingjie","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hyung-Yong","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joe","family":"Rodriguez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shien","family":"Tsai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shyh-Ching","family":"Lo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","published-online":{"date-parts":[[2011,3,30]]},"reference":[{"key":"key20180919073514_B1","doi-asserted-by":"crossref","first-page":"551","DOI":"10.1038\/sj.icb.7100084","article-title":"Development of protective immunity in a murine model of melioidosis is influenced by the source of Burkholderia pseudomallei antigens","volume":"85","author":"Barnes","year":"2007","journal-title":"Immunol. 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