{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,14]],"date-time":"2025-10-14T06:57:31Z","timestamp":1760425051015},"reference-count":36,"publisher":"American Society for Microbiology","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clin. Vaccine Immunol."],"published-print":{"date-parts":[[2011,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>The detection of<jats:named-content content-type=\"genus-species\">Trypanosoma cruzi<\/jats:named-content>infection in domestic dogs and cats is relevant to evaluating human transmission risks and the effectiveness of insecticide spraying campaigns. However, the serological assays routinely used are associated with cross-reactivity in sera from mammals infected with<jats:named-content content-type=\"genus-species\">Leishmania<\/jats:named-content>spp. We used a<jats:italic>trans<\/jats:italic>-sialidase inhibition assay (TIA) for<jats:named-content content-type=\"genus-species\">T. cruzi<\/jats:named-content>diagnosis in serum samples from 199 dogs and 57 cats from areas where these types of infections are endemic. TIA is based on the antibody neutralization of recombinant<jats:italic>trans<\/jats:italic>-sialidase, an enzyme that is not detected in the coendemic<jats:named-content content-type=\"genus-species\">Leishmania<\/jats:named-content>species or<jats:named-content content-type=\"genus-species\">Trypanosoma rangeli<\/jats:named-content>parasites.<jats:named-content content-type=\"genus-species\">T. cruzi<\/jats:named-content>infection was also evaluated by conventional serology (CS) (indirect immunofluorescence, indirect hemagglutination, enzyme-linked immunosorbent assay, and immunochromatographic dipstick test) and xenodiagnosis. Sera from 30 dogs and 15 cats from areas where these organisms are not endemic and 5 dogs with visceral leishmaniasis were found to be nonreactive by TIA and CS. Samples from dogs and cats demonstrated 91 and 95% copositivities between TIA and CS, whereas the conegativities were 98 and 97%, respectively. Sera from xenodiagnosis-positive dogs and cats also reacted by TIA (copositivities of 97 and 83%, respectively). TIA was reactive in three CS-negative samples and was able to resolve results in two cat serum samples that were CS inconclusive. Our study is the first to describe the development of<jats:italic>trans<\/jats:italic>-sialidase neutralizing antibodies in naturally infected dogs and cats. High CS conegativity and the absence of<jats:italic>trans-<\/jats:italic>sialidase neutralization in dog sera from areas where leishmaniasis is not endemic and from dogs with visceral leishmaniasis support TIA specificity. The TIA may be a useful tool for<jats:named-content content-type=\"genus-species\">T. cruzi<\/jats:named-content>detection in the main domestic reservoirs.<\/jats:p>","DOI":"10.1128\/cvi.00535-10","type":"journal-article","created":{"date-parts":[[2011,4,7]],"date-time":"2011-04-07T04:39:35Z","timestamp":1302151175000},"page":"984-989","source":"Crossref","is-referenced-by-count":10,"title":["<i>trans<\/i>-Sialidase Neutralizing Antibody Detection in Trypanosoma cruzi-Infected Domestic Reservoirs"],"prefix":"10.1128","volume":"18","author":[{"given":"Paula A.","family":"Sartor","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martha V.","family":"Cardinal","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcela M.","family":"Orozco","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ricardo E.","family":"G\u00fcrtler","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. 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Microbiol."},{"key":"key20180919073518_B7","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1016\/j.actatropica.2007.06.007","article-title":"Impact of community-based vector control on house infestation and Trypanosoma cruzi infection in Triatoma infestans, dogs and cats in the Argentine Chaco","volume":"103","author":"Cardinal","year":"2007","journal-title":"Acta Trop."},{"key":"key20180919073518_B8","doi-asserted-by":"crossref","first-page":"1533","DOI":"10.1016\/j.ijpara.2008.04.010","article-title":"Molecular epidemiology of domestic and sylvatic Trypanosoma cruzi infection in rural northwestern Argentina","volume":"38","author":"Cardinal","year":"2008","journal-title":"Int. J. Parasitol."},{"key":"key20180919073518_B9","doi-asserted-by":"crossref","first-page":"671","DOI":"10.1080\/00034983.1998.11813327","article-title":"Evaluation of dogs as sentinels of the transmission of Trypanosoma cruzi in a rural area of northwestern Argentina","volume":"92","author":"Casta\u00f1era","year":"1998","journal-title":"Ann. Trop. Med. 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Inst. Oswaldo Cruz"},{"key":"key20180919073518_B21","doi-asserted-by":"crossref","first-page":"3441","DOI":"10.1128\/IAI.62.8.3441-3446.1994","article-title":"Mice infected with Trypanosoma cruzi produce antibodies against the enzymatic domain of trans-sialidase that inhibit its activity","volume":"62","author":"Leguizam\u00f3n","year":"1994","journal-title":"Infect. Immun."},{"key":"key20180919073518_B22","doi-asserted-by":"crossref","first-page":"1570","DOI":"10.1093\/infdis\/170.6.1570","article-title":"Antibodies inhibiting Trypanosoma cruzi trans-sialidase activity in sera from human infections","volume":"170","author":"Leguizam\u00f3n","year":"1994","journal-title":"J. Infect. Dis."},{"key":"key20180919073518_B23","doi-asserted-by":"crossref","first-page":"254","DOI":"10.1128\/CDLI.5.2.254-255.1998","article-title":"Use of trans-sialidase inhibition assay in a population serologically negative for Trypanosoma cruzi but at high risk of infection","volume":"5","author":"Leguizam\u00f3n","year":"1998","journal-title":"Clin. Diagn. Lab. Immunol."},{"key":"key20180919073518_B24","doi-asserted-by":"crossref","first-page":"302","DOI":"10.1016\/j.ab.2006.07.018","article-title":"Continuous fluorimetric assay for high-throughput screening of inhibitors of trans-sialidase from Trypanosoma cruzi","volume":"357","author":"Neres","year":"2006","journal-title":"Anal. Biochem."},{"key":"key20180919073518_B25","doi-asserted-by":"crossref","first-page":"1705","DOI":"10.1002\/j.1460-2075.1992.tb05221.x","article-title":"Identification of the gene(s) coding for the trans-sialidase of Trypanosoma cruzi","volume":"11","author":"Parodi","year":"1992","journal-title":"EMBO J."},{"key":"key20180919073518_B26","doi-asserted-by":"crossref","first-page":"2973","DOI":"10.1128\/IAI.62.7.2973-2978.1994","article-title":"Sera from chronic patients and rodents infected with Trypanosoma cruzi inhibit trans-sialidase by recognizing its amino-terminal and catalytic domain","volume":"62","author":"Pereira-Chioccola","year":"1994","journal-title":"Infect. Immun."},{"key":"key20180919073518_B27","doi-asserted-by":"crossref","first-page":"208","DOI":"10.1007\/s004360000324","article-title":"Association between nutritional indicators and infectivity of dogs seroreactive for Trypanosoma cruzi in a rural area of northwestern Argentina","volume":"87","author":"Petersen","year":"2001","journal-title":"Parasitol. Res."},{"key":"key20180919073518_B28","doi-asserted-by":"crossref","first-page":"1915","DOI":"10.1017\/S0031182009991156","article-title":"Transmission, reservoir hosts and control of zoonotic visceral leishmaniasis","volume":"136","author":"Quinnell","year":"2009","journal-title":"Parasitology"},{"key":"key20180919073518_B29","doi-asserted-by":"crossref","first-page":"2250","DOI":"10.1086\/420831","article-title":"Differential expression of a virulence factor, the trans-sialidase, by the main Trypanosoma cruzi phylogenetic lineages","volume":"189","author":"Risso","year":"2004","journal-title":"J. Infect. 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Biochem."},{"key":"key20180919073518_B33","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1128\/CDLI.11.2.313-319.2004","article-title":"Combined use of enzyme-linked immunosorbent assay and flow cytometry to detect antibodies to Trypanosoma cruzi in domestic canines in Texas","volume":"11","author":"Shadomy","year":"2004","journal-title":"Clin. Diagn. Lab. Immunol."},{"key":"key20180919073518_B34","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/j.actatropica.2009.01.006","article-title":"TESA-Blot for the diagnosis of Chagas disease in dogs from co-endemic regions for Trypanosoma cruzi, Trypanosoma evansi, and Leishmania chagasi","volume":"111","author":"Umezawa","year":"2009","journal-title":"Acta Trop."},{"key":"key20180919073518_B35","doi-asserted-by":"crossref","first-page":"528","DOI":"10.1590\/S0074-02762008000600003","article-title":"Trypanosoma cruzi: blood parasitism kinetics and their correlation with heart parasitism intensity during long-term infection of Beagle dogs","volume":"103","author":"Veloso","year":"2008","journal-title":"Mem. Inst. Oswaldo Cruz"},{"key":"key20180919073518_B36","first-page":"82","article-title":"Control of Chagas disease","volume":"905","author":"World Health Organization","year":"2002","journal-title":"WHO Tech. Rep. 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