{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,20]],"date-time":"2026-02-20T18:27:51Z","timestamp":1771612071132,"version":"3.50.1"},"reference-count":21,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2017,5,1]],"date-time":"2017-05-01T00:00:00Z","timestamp":1493596800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100000277","name":"Department for Environment, Food and Rural Affairs","doi-asserted-by":"publisher","award":["SE3224"],"award-info":[{"award-number":["SE3224"]}],"id":[{"id":"10.13039\/501100000277","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000277","name":"Department for Environment, Food and Rural Affairs","doi-asserted-by":"publisher","award":["SE3227"],"award-info":[{"award-number":["SE3227"]}],"id":[{"id":"10.13039\/501100000277","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000277","name":"Department for Environment, Food and Rural Affairs","doi-asserted-by":"publisher","award":["SE3266"],"award-info":[{"award-number":["SE3266"]}],"id":[{"id":"10.13039\/501100000277","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000277","name":"Department for Environment, Food and Rural Affairs","doi-asserted-by":"publisher","award":["SE3268"],"award-info":[{"award-number":["SE3268"]}],"id":[{"id":"10.13039\/501100000277","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Vaccine Immunol"],"published-print":{"date-parts":[[2017,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:named-content content-type=\"genus-species\">Mycobacterium bovis<\/jats:named-content>\n            BCG vaccination sensitizes cattle to bovine tuberculin, which compromises the use of the current bovine tuberculosis (TB) surveillance tests. Although the performance of a blood test (that utilizes antigens expressed by\n            <jats:named-content content-type=\"genus-species\">Mycobacterium bovis<\/jats:named-content>\n            but not by BCG) capable of discriminating infected from vaccinated animals (DIVA interferon gamma test [DIT]) has been evaluated in naturally infected TB field reactors, there is a need to perform similar analysis in a BCG-vaccinated\n            <jats:named-content content-type=\"genus-species\">M. bovis<\/jats:named-content>\n            -infected population. Furthermore, we explored different scenarios under which a DIT may be implemented alongside BCG vaccination: (i) serial testing to resolve potential false-positive skin test results or (ii) a standalone test to replace the single intradermal comparative cervical tuberculin (SICCT) skin test. Our results demonstrated significantly better relative test sensitivity when the DIT was evaluated in a serial test scenario. Direct comparison of pre- and post-skin test blood samples revealed that the SICCT test induced significant boosting of the gamma interferon response in\n            <jats:named-content content-type=\"genus-species\">M. bovis<\/jats:named-content>\n            -infected animals to both the ESAT-6\u2013CFP-10 and Rv3615c peptide cocktails that comprise the DIT, which persisted for the ESAT-6\u2013CFP-10 reagent for at least 14 days. Importantly, no similar boosting effects were observed in noninfected BCG vaccinates, suggesting that DIVA blood testing after a recent skin test would have minimal impact on test specificity.\n          <\/jats:p>","DOI":"10.1128\/cvi.00551-16","type":"journal-article","created":{"date-parts":[[2017,3,23]],"date-time":"2017-03-23T00:33:57Z","timestamp":1490229237000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":11,"title":["Tuberculin Skin Testing Boosts Interferon Gamma Responses to DIVA Reagents in Mycobacterium bovis-Infected Cattle"],"prefix":"10.1128","volume":"24","author":[{"given":"Gareth J.","family":"Jones","sequence":"first","affiliation":[{"name":"TB Immunology and Vaccinology, Department of Bacteriology, Animal and Plant Health Agency, New Haw, Addlestone, Surrey, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mick","family":"Coad","sequence":"additional","affiliation":[{"name":"TB Immunology and Vaccinology, Department of Bacteriology, Animal and Plant Health Agency, New Haw, Addlestone, Surrey, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bhagwati","family":"Khatri","sequence":"additional","affiliation":[{"name":"TB Immunology and Vaccinology, Department of Bacteriology, Animal and Plant Health Agency, New Haw, Addlestone, Surrey, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Javier","family":"Bezos","sequence":"additional","affiliation":[{"name":"AEVA-SERVET S.L., Madrid, Spain"},{"name":"Centro de Vigilancia Sanitaria Veterinaria (VISAVET), Universidad Complutense, Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Natalie A.","family":"Parlane","sequence":"additional","affiliation":[{"name":"AgResearch, Hopkirk Research Institute, Palmerston North, New Zealand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bryce M.","family":"Buddle","sequence":"additional","affiliation":[{"name":"AgResearch, Hopkirk Research Institute, Palmerston North, New Zealand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bernardo","family":"Villarreal-Ramos","sequence":"additional","affiliation":[{"name":"TB Immunology and Vaccinology, Department of Bacteriology, Animal and Plant Health Agency, New Haw, Addlestone, Surrey, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. Glyn","family":"Hewinson","sequence":"additional","affiliation":[{"name":"TB Immunology and Vaccinology, Department of Bacteriology, Animal and Plant Health Agency, New Haw, Addlestone, Surrey, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H. Martin","family":"Vordermeier","sequence":"additional","affiliation":[{"name":"TB Immunology and Vaccinology, Department of Bacteriology, Animal and Plant Health Agency, New Haw, Addlestone, Surrey, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2012.02.018"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00435-13"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2013.11.045"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00301-12"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00150-08"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/CDLI.8.3.571-578.2001"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/0264-410X(94)00055-R"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.11.6411-6419.2003"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00543-10"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2249.2005.02668.x"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetimm.2007.04.005"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.6.3026-3032.2002"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetimm.2010.02.007"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2011.05.057"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.rvsc.2005.11.005"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1135(94)90050-7"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0043217"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2014.02.036"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.5.2462-2467.2004"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0142270"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0106519"}],"container-title":["Clinical and Vaccine Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/CVI.00551-16","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/CVI.00551-16","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,6,3]],"date-time":"2021-06-03T02:22:38Z","timestamp":1622686958000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/CVI.00551-16"}},"subtitle":[],"editor":[{"given":"Christopher J.","family":"Papasian","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2017,5]]},"references-count":21,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2017,5]]}},"alternative-id":["10.1128\/CVI.00551-16"],"URL":"https:\/\/doi.org\/10.1128\/cvi.00551-16","relation":{},"ISSN":["1556-6811","1556-679X"],"issn-type":[{"value":"1556-6811","type":"print"},{"value":"1556-679X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,5]]},"assertion":[{"value":"2017-01-11","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-03-17","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-05-05","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}