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Intranasal coadministration of BPPcysMPEG with a plasmid carrying the SOD-encoding gene (pcDNA-SOD) into BALB\/c mice elicited antigen-specific humoral and cellular immune responses. Humoral responses were characterized by the stimulation of IgG2a and IgG1 and by the presence of SOD-specific secretory IgA in nasal and bronchoalveolar lavage fluids. Furthermore, T-cell proliferative responses and increased production of gamma interferon were also observed upon splenocyte restimulation with recombinant SOD. Cytotoxic responses were also stimulated, as demonstrated by the lysis of RB51-SOD-infected J774.A1 macrophages by cells recovered from immunized mice. The pcDNA-SOD\/BPPcysMPEG formulation induced improved protection against challenge with the virulent strain<jats:named-content content-type=\"genus-species\">B. abortus<\/jats:named-content>2308 in BALB\/c mice over that provided by pcDNA-SOD, suggesting the potential of this vaccination strategy against<jats:named-content content-type=\"genus-species\">Brucella<\/jats:named-content>infection.<\/jats:p>","DOI":"10.1128\/cvi.00554-14","type":"journal-article","created":{"date-parts":[[2014,8,28]],"date-time":"2014-08-28T04:25:10Z","timestamp":1409199910000},"page":"1474-1480","source":"Crossref","is-referenced-by-count":10,"title":["Use of<i>S<\/i>-[2,3-Bispalmitoyiloxy-(2<i>R<\/i>)-Propyl]-<i>R<\/i>-Cysteinyl-Amido-Monomethoxy Polyethylene Glycol as an Adjuvant Improved Protective Immunity Associated with a DNA Vaccine Encoding Cu,Zn Superoxide Dismutase of Brucella abortus in Mice"],"prefix":"10.1128","volume":"21","author":[{"given":"Angello","family":"Retamal-D\u00edaz","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roberto","family":"Riquelme-Neira","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Darwin","family":"S\u00e1ez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alejandra","family":"Rivera","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pablo","family":"Fern\u00e1ndez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alex","family":"Cabrera","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carlos A.","family":"Guzm\u00e1n","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"\u00c1ngel","family":"O\u00f1ate","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","published-online":{"date-parts":[[2014,8,27]]},"reference":[{"key":"key20180919073748_B1","doi-asserted-by":"publisher","first-page":"213","DOI":"10.3201\/eid0302.970219","article-title":"Brucellosis: an overview","volume":"3","author":"Corbel","year":"1997","journal-title":"Emerg. 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