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In mice, the survival times of the four mutants were very different in the virulence assay, from less than 6 weeks for<jats:named-content content-type=\"genus-species\">B. abortus<\/jats:named-content>S19-RB6 to 11 weeks for<jats:named-content content-type=\"genus-species\">B. abortus<\/jats:named-content>2308-SB6 and<jats:named-content content-type=\"genus-species\">B. melitensis<\/jats:named-content>NI-NB6. However,<jats:named-content content-type=\"genus-species\">B. abortus<\/jats:named-content>S19-RB6 and<jats:named-content content-type=\"genus-species\">B. melitensis<\/jats:named-content>16 M-MB6, with a shorter survival time in mice, offered better protection against challenges with<jats:named-content content-type=\"genus-species\">B. abortus<\/jats:named-content>2308 in protection tests than<jats:named-content content-type=\"genus-species\">B. abortus<\/jats:named-content>2308-SB6 and<jats:named-content content-type=\"genus-species\">B. melitensis<\/jats:named-content>NI-NB6. It seems that the induced protective immunity of each mutant might not be associated with its survival time<jats:italic>in vivo<\/jats:italic>. In the cross-protection assay, both<jats:named-content content-type=\"genus-species\">B. melitensis<\/jats:named-content>16 M-MB6 and<jats:named-content content-type=\"genus-species\">B. abortus<\/jats:named-content>S19-RB6 induced greater protection against homologous challenges than heterologous challenges. When pregnant sheep were inoculated with<jats:named-content content-type=\"genus-species\">B. abortus<\/jats:named-content>S19-RB6 and<jats:named-content content-type=\"genus-species\">B. melitensis<\/jats:named-content>16 M-MB6,<jats:named-content content-type=\"genus-species\">B. abortus<\/jats:named-content>S19-RB6 did not induce abortion, whereas<jats:named-content content-type=\"genus-species\">B. melitensis<\/jats:named-content>16 M-MB6 did. These results demonstrated the differences in virulence, pathogenicity, and protective immunity<jats:italic>in vivo<\/jats:italic>in the<jats:italic>wboA<\/jats:italic>deletion mutants from genetically different parent<jats:named-content content-type=\"genus-species\">Brucella<\/jats:named-content>spp. and also indicated that future rough vaccine strain development could be promising if suitable parent<jats:named-content content-type=\"genus-species\">Brucella<\/jats:named-content>strains and\/or genes were selected.<\/jats:p>","DOI":"10.1128\/cvi.00573-12","type":"journal-article","created":{"date-parts":[[2012,12,13]],"date-time":"2012-12-13T04:56:49Z","timestamp":1355374609000},"page":"174-180","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":12,"title":["<i>In Vivo<\/i>Differences in the Virulence, Pathogenicity, and Induced Protective Immunity of<i>wboA<\/i>Mutants from Genetically Different Parent Brucella spp"],"prefix":"10.1128","volume":"20","author":[{"given":"Zhen","family":"Wang","sequence":"first","affiliation":[{"name":"Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianrui","family":"Niu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuangshan","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanli","family":"Lv","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingmin","family":"Wu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid0302.970219"},{"key":"e_1_3_2_3_2","first-page":"125","article-title":"Brucellosis in human beings","volume":"49","author":"Rice TB","year":"1946","unstructured":"RiceTB. 1946. 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