{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,3]],"date-time":"2025-11-03T04:42:52Z","timestamp":1762144972942},"reference-count":36,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2013,12,1]],"date-time":"2013-12-01T00:00:00Z","timestamp":1385856000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Vaccine Immunol"],"published-print":{"date-parts":[[2013,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Diagnosing canine visceral leishmaniasis (CVL) is a critical challenge since conventional immunoserological tests still present some deficiencies. The current study evaluated a prototype flow cytometry serology test, using antigens and fluorescent antibodies that had been stored for 1 year at 4\u00b0C, on a broad range of serum samples. Noninfected control dogs and\n            <jats:named-content content-type=\"genus-species\">Leishmania infantum<\/jats:named-content>\n            -infected dogs were tested, and the prototype test showed excellent performance in differentiating these groups with high sensitivity, specificity, positive and negative predictive values, and accuracy (100% in all analyses). When the CVL group was evaluated according to the dogs' clinical status, the prototype test showed outstanding accuracy in all groups with positive serology (asymptomatic II, oligosymptomatic, and symptomatic). However, in dogs which had positive results by PCR-restriction fragment length polymorphism (RFLP) but negative results by conventional serology (asymptomatic I), serological reactivity was not observed. Additionally, sera from 40 dogs immunized with different vaccines (Leishmune, Leish-Tec, or LBSap) did not present serological reactivity in the prototype test. Eighty-eight dogs infected with other pathogens (\n            <jats:named-content content-type=\"genus-species\">Trypanosoma cruzi<\/jats:named-content>\n            ,\n            <jats:named-content content-type=\"genus-species\">Leishmania braziliensis<\/jats:named-content>\n            ,\n            <jats:named-content content-type=\"genus-species\">Ehrlichia canis<\/jats:named-content>\n            , and\n            <jats:named-content content-type=\"genus-species\">Babesia canis<\/jats:named-content>\n            ) were used to determine cross-reactivity and specificity, and the prototype test performed well, particularly in dogs infected with\n            <jats:named-content content-type=\"genus-species\">B. canis<\/jats:named-content>\n            and\n            <jats:named-content content-type=\"genus-species\">E. canis<\/jats:named-content>\n            (100% and 93.3% specificities, respectively). In conclusion, our data reinforce the potential of the prototype test for use as a commercial kit and highlight its outstanding performance even after storage for 1 year at 4\u00b0C. Moreover, the prototype test efficiently provided accurate CVL serodiagnosis with an absence of false-positive results in vaccinated dogs and minor cross-reactivity against other canine pathogens.\n          <\/jats:p>","DOI":"10.1128\/cvi.00575-13","type":"journal-article","created":{"date-parts":[[2013,10,10]],"date-time":"2013-10-10T14:56:39Z","timestamp":1381416999000},"page":"1792-1798","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":14,"title":["Evaluation of a Prototype Flow Cytometry Test for Serodiagnosis of Canine Visceral Leishmaniasis"],"prefix":"10.1128","volume":"20","author":[{"given":"Henrique Gama","family":"Ker","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de Pesquisas Cl\u00ednicas, Programa de P\u00f3s Gradua\u00e7\u00e3o em Ci\u00eancias Farmac\u00eauticas, Universidade Federal de Ouro Preto, Ouro Preto, Minas Gerais, Brazil"},{"name":"Laborat\u00f3rio de Imunopatologia, N\u00facleo de Pesquisas em Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Ouro Preto, Ouro Preto, Minas Gerais, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wendel","family":"Coura-Vital","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Pesquisas Cl\u00ednicas, Programa de P\u00f3s Gradua\u00e7\u00e3o em Ci\u00eancias Farmac\u00eauticas, Universidade Federal de Ouro Preto, Ouro Preto, Minas Gerais, Brazil"},{"name":"P\u00f3s-Gradua\u00e7\u00e3o em Infectologia e Medicina Tropical, Faculdade de Medicina, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rodrigo Dian de Oliveira","family":"Aguiar-Soares","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Pesquisas Cl\u00ednicas, Programa de P\u00f3s Gradua\u00e7\u00e3o em Ci\u00eancias Farmac\u00eauticas, Universidade Federal de Ouro Preto, Ouro Preto, Minas Gerais, Brazil"},{"name":"Laborat\u00f3rio de Imunopatologia, N\u00facleo de Pesquisas em Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Ouro Preto, Ouro Preto, Minas Gerais, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bruno Mendes","family":"Roatt","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Pesquisas Cl\u00ednicas, Programa de P\u00f3s Gradua\u00e7\u00e3o em Ci\u00eancias Farmac\u00eauticas, Universidade Federal de Ouro Preto, Ouro Preto, Minas Gerais, Brazil"},{"name":"Laborat\u00f3rio de Imunopatologia, N\u00facleo de Pesquisas em Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Ouro Preto, Ouro Preto, Minas Gerais, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N\u00e1dia","family":"das Dores Moreira","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Pesquisas Cl\u00ednicas, Programa de P\u00f3s Gradua\u00e7\u00e3o em Ci\u00eancias Farmac\u00eauticas, Universidade Federal de Ouro Preto, Ouro Preto, Minas Gerais, Brazil"},{"name":"Laborat\u00f3rio de Imunopatologia, N\u00facleo de Pesquisas em Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Ouro Preto, Ouro Preto, Minas Gerais, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cl\u00e1udia Martins","family":"Carneiro","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Pesquisas Cl\u00ednicas, Programa de P\u00f3s Gradua\u00e7\u00e3o em Ci\u00eancias Farmac\u00eauticas, Universidade Federal de Ouro Preto, Ouro Preto, Minas Gerais, Brazil"},{"name":"Laborat\u00f3rio de Imunopatologia, N\u00facleo de Pesquisas em Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Ouro Preto, Ouro Preto, Minas Gerais, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Evandro Marques de Menezes","family":"Machado","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Imunopatologia, N\u00facleo de Pesquisas em Ci\u00eancias Biol\u00f3gicas, 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