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The present study describes a sequential subtractive selection through phage display technology from polyclonal antibodies of negative and positive sera that resulted in the identification of potential bacteriophage-fused peptides that were highly sensitive and specific to antibodies of CVL. A negative selection was performed in which phage clones were adhered to purified IgGs from healthy and<jats:named-content content-type=\"genus-species\">Trypanosoma cruzi<\/jats:named-content>-infected dogs to eliminate cross-reactive phages. The remaining supernatant nonadhered phages were submitted to positive selection against IgG from the blood serum of dogs that were infected with<jats:named-content content-type=\"genus-species\">Leishmania infantum<\/jats:named-content>. Phage clones that adhered to purified IgGs from the CVL-infected serum samples were selected. Eighteen clones were identified and their reactivities tested by a phage enzyme-linked immunosorbent assay (phage-ELISA) against the serum samples from infected dogs (<jats:italic>n<\/jats:italic>= 31) compared to those from vaccinated dogs (<jats:italic>n<\/jats:italic>= 21), experimentally infected dogs with cross-reactive parasites (<jats:italic>n<\/jats:italic>= 23), and healthy controls (<jats:italic>n<\/jats:italic>= 17). Eight clones presented sensitivity, specificity, and positive and negative predictive values of 100%, and they showed no cross-reactivity with<jats:named-content content-type=\"genus-species\">T. cruzi<\/jats:named-content>- or<jats:named-content content-type=\"genus-species\">Ehrlichia canis<\/jats:named-content>-infected dogs or with dogs vaccinated with two different commercial CVL vaccines in Brazil. Our study identified eight mimotopes of<jats:named-content content-type=\"genus-species\">L. infantum<\/jats:named-content>antigens with 100% accuracy for CVL serodiagnosis. The use of these mimotopes by phage-ELISA proved to be an excellent assay that was reproducible, simple, fast, and inexpensive, and it can be applied in CVL-monitoring programs.<\/jats:p>","DOI":"10.1128\/cvi.00583-13","type":"journal-article","created":{"date-parts":[[2013,11,21]],"date-time":"2013-11-21T04:40:44Z","timestamp":1385008844000},"page":"96-106","source":"Crossref","is-referenced-by-count":26,"title":["Subtractive Phage Display Selection from Canine Visceral Leishmaniasis Identifies Novel Epitopes That Mimic Leishmania infantum Antigens with Potential Serodiagnosis Applications"],"prefix":"10.1128","volume":"21","author":[{"given":"Lourena E.","family":"Costa","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mayara I. 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