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The attenuated DPV vaccine strain CHa is a modified live vaccine, but the systemic and mucosal immune responses induced by this vaccine have been poorly understood. In this study, the immunogenicity and efficacy of the vaccine were evaluated after subcutaneous immunization of ducks. CD4<jats:sup>+<\/jats:sup>and CD8<jats:sup>+<\/jats:sup>T cells were counted by flow cytometry, and humoral and mucosal Ig antibodies were analyzed by enzyme-linked immunosorbent assay (ELISA). The results showed that high levels of T cells and Ig antibodies were present postimmunization and that there were more CD4<jats:sup>+<\/jats:sup>T cells than CD8<jats:sup>+<\/jats:sup>T cells. Titers of humoral IgG were higher than those of humoral IgA. Local IgA was found in each sample, whereas local IgG was found only in the spleen, thymus, bursa of Fabricius, harderian gland, liver, bile, and lung. In a protection assay, the attenuated DPV vaccine completely protected ducks against 1,000 50% lethal doses (LD<jats:sub>50<\/jats:sub>) of the lethal DPV strain CHv via oral infection. These data suggest that this subcutaneous vaccine elicits sufficient systemic and mucosal immune responses against lethal DPV challenge to be protective in ducks. This study provides broad insights into understanding the immune responses to the attenuated DPV vaccine strain CHa through subcutaneous immunization in ducks.<\/jats:p>","DOI":"10.1128\/cvi.00605-13","type":"journal-article","created":{"date-parts":[[2014,1,23]],"date-time":"2014-01-23T04:26:50Z","timestamp":1390451210000},"page":"457-462","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":30,"title":["An Attenuated Duck Plague Virus (DPV) Vaccine Induces both Systemic and Mucosal Immune Responses To Protect Ducks against Virulent DPV Infection"],"prefix":"10.1128","volume":"21","author":[{"given":"Juan","family":"Huang","sequence":"first","affiliation":[{"name":"Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, People's Republic of China"},{"name":"Avian Disease Research Center, College of Veterinary Medicine of Sichuan Agricultural University, Ya'an, Sichuan, People's 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