{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T15:28:33Z","timestamp":1789918113894,"version":"4.0.1"},"reference-count":19,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2014,4,1]],"date-time":"2014-04-01T00:00:00Z","timestamp":1396310400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Vaccine Immunol"],"published-print":{"date-parts":[[2014,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>The BioPlex 2200 automated analyzer (Bio-Rad Laboratories, Hercules, CA) is a recently developed multiplex analyzer that enables the detection of anti-<jats:named-content content-type=\"genus-species\">Toxoplasma<\/jats:named-content>, -rubella, and -cytomegalovirus antibodies in the same assay. The aim of this study was to compare this new technology (using the BioPlex 2200 ToRC IgG\/IgM kit) in critical cases of serodiagnosis of toxoplasmosis (acute, chronic, or congenital infections and cases with discrepant results) to the technologies used in our routine practice, i.e., the Platelia IgG\/IgM enzyme-linked immunosorbent assays (ELISAs) (Bio-Rad Laboratories) and the Toxo-Screen direct agglutination assay (bioM\u00e9rieux, Lyon, France). Overall, most cases of false-positive\/negative results obtained with the Platelia IgG or Toxo-Screen assay were corrected by the BioPlex 2200 ToRC IgG (87.5%). Furthermore, the analysis of 35 sequences of sera showed a trend toward a more rapid decrease of IgM titers by BioPlex 2200 than by Platelia. These results for IgM detection can be explained by a weaker detection of residual IgM. Indeed, among 23 serum samples from patients with probable past infection with long-lasting IgM (Platelia M positive and IgG avidity index, \u22650.5), the BioPlex 2200<jats:named-content content-type=\"genus-species\">Toxoplasma<\/jats:named-content>IgM assay was positive for only 11 serum samples. In our panel of critical cases comprising 156 serum and 6 cord blood samples from 103 patients with acute, chronic, or congenital infection, the BioPlex 2200 IgG assay was a sensitive (97.8%) and specific (91.3%) method for IgG detection. The high specificity (97.4%) of IgM detection combined with the shorter kinetics of IgM titers may considerably reduce the number of residual IgM detections, thus yielding more precise diagnoses of acute infections.<\/jats:p>","DOI":"10.1128\/cvi.00624-13","type":"journal-article","created":{"date-parts":[[2014,1,30]],"date-time":"2014-01-30T04:50:27Z","timestamp":1391057427000},"page":"496-500","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":18,"title":["Performance of the BioPlex 2200 Flow Immunoassay in Critical Cases of Serodiagnosis of Toxoplasmosis"],"prefix":"10.1128","volume":"21","author":[{"given":"Nicolas","family":"Guigue","sequence":"first","affiliation":[{"name":"Laboratoire de Parasitologie et de Mycologie, H\u00f4pital Saint Louis, Assistance Publique-H\u00f4pitaux de Paris et Universit\u00e9 Paris Diderot, Sorbonne Paris Cit\u00e9, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean","family":"Menotti","sequence":"additional","affiliation":[{"name":"Laboratoire de Parasitologie et de Mycologie, H\u00f4pital Saint Louis, Assistance Publique-H\u00f4pitaux de Paris et Universit\u00e9 Paris Diderot, Sorbonne Paris Cit\u00e9, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samia","family":"Hamane","sequence":"additional","affiliation":[{"name":"Laboratoire de Parasitologie et de Mycologie, H\u00f4pital Saint Louis, Assistance Publique-H\u00f4pitaux de Paris et Universit\u00e9 Paris Diderot, Sorbonne Paris Cit\u00e9, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francis","family":"Derouin","sequence":"additional","affiliation":[{"name":"Laboratoire de Parasitologie et de Mycologie, H\u00f4pital Saint Louis, Assistance Publique-H\u00f4pitaux de Paris et Universit\u00e9 Paris Diderot, Sorbonne Paris Cit\u00e9, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yves Jean-Fran\u00e7ois","family":"Garin","sequence":"additional","affiliation":[{"name":"Laboratoire de Parasitologie et de Mycologie, H\u00f4pital Saint Louis, Assistance Publique-H\u00f4pitaux de Paris et Universit\u00e9 Paris Diderot, Sorbonne Paris Cit\u00e9, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.42.2.191"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1086\/338827"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00128-09"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2012.03.014"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2009.07.013"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00332-10"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00082-08"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00086-10"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(02)00242-6"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.12.5705-5709.2004"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00182-08"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.05303-11"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00356-12"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.clim.2003.11.017"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-59745-323-3_9"},{"key":"e_1_3_2_17_2","first-page":"360","article-title":"Understanding interobserver agreement: the kappa statistic","volume":"37","author":"Viera AJ","year":"2005","unstructured":"VieraAJGarrettJM. 2005. Understanding interobserver agreement: the kappa statistic. Fam. Med. 37:360\u2013363.","journal-title":"Fam. Med."},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.3.941-945.2004"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1086\/590149"},{"key":"e_1_3_2_20_2","doi-asserted-by":"crossref","first-page":"786","DOI":"10.4103\/0377-4929.91503","article-title":"Laboratory diagnosis of Toxoplasma gondii infection with direct and indirect diagnostic techniques","volume":"54","author":"Pignanelli S","year":"2011","unstructured":"PignanelliS. 2011. Laboratory diagnosis of Toxoplasma gondii infection with direct and indirect diagnostic techniques. Indian J. Pathol. Microbiol. 54:786\u2013789.","journal-title":"Indian J. Pathol. Microbiol."}],"container-title":["Clinical and Vaccine Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/CVI.00624-13","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/CVI.00624-13","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,5,1]],"date-time":"2025-05-01T18:16:36Z","timestamp":1746123396000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/CVI.00624-13"}},"subtitle":[],"editor":[{"given":"P. P.","family":"Wilkins","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2014,4]]},"references-count":19,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2014,4]]}},"alternative-id":["10.1128\/CVI.00624-13"],"URL":"https:\/\/doi.org\/10.1128\/cvi.00624-13","relation":{},"ISSN":["1556-6811","1556-679X"],"issn-type":[{"value":"1556-6811","type":"print"},{"value":"1556-679X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,4]]},"assertion":[{"value":"2013-10-08","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2014-01-22","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2014-03-26","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}